Particle damping technology has attracted extensive research and engineering application interest in the field of vibration control due to its prominent advantages, including wide working frequency bands, ease of installation, longer durability and insensitivity to extreme temperatures. To introduce particle damping technology to long-period structure seismic control, a novel multilayer compartmental particle damper (MCPD) was proposed, and a 1/20 scale test model of a typical long-period self-anchored suspension bridge with a single tower was designed and fabricated. The model was subjected to a series of shaking table tests with and without the MCPD. The results showed that the seismic responses of the flexible or semi-flexible bridge towers of long-period bridges influence the seismic responses of the main beam. The MCPD can be conveniently installed on the main beam and bridge tower and can effectively reduce the longitudinal peak displacement and the root mean square acceleration of the main beam and tower. In addition, no particle accumulation was observed during the tests. A well-designed MCPD can achieve significant damping for long-period structures under seismic excitations of different intensities. These results indicate that the application of MCPDs for seismic control of single-tower self-anchored suspension bridges and other long-period structures is viable.