[ 1 ] Andrianantoandro E, Basu S, Karig DK, Weiss R. Synthetic biology: new engineering rules for an emerging discipline. Mol Syst Biol 2006;2 (1):2006.0028. 链接1

[ 2 ] Choffnes ER, Relman DA, Pray L. The science and applications of synthetic and systems biology: workshop summary. Report. Washington, DC: National Academies Press; 2011. 链接1

[ 3 ] Ramon A, Smith HO. Single-step linker-based combinatorial assembly of promoter and gene cassettes for pathway engineering. Biotechnol Lett 2011;33 (3):549–55. 链接1

[ 4 ] Wingler LM, Cornish VW. Reiterative recombination for the in vivo assembly of libraries of multigene pathways. Proc Natl Acad Sci USA 2011;108 (37):15135–40. 链接1

[ 5 ] Dietrich JA, McKee AE, Keasling JD. High-throughput metabolic engineering: advances in small-molecule screening and selection. Annu Rev Biochem 2010;79(1):563–90. 链接1

[ 6 ] Purnick PEM, Weiss R. The second wave of synthetic biology: from modules to systems. Nat Rev Mol Cell Biol 2009;10(6):410–22. 链接1

[ 7 ] Nielsen AAK, Der BS, Shin J, Vaidyanathan P, Paralanov V, Strychalski EA, et al. Genetic circuit design automation. Science 2016;352(6281):aac7341. 链接1

[ 8 ] Chao R, Yuan Y, Zhao H. Recent advances in DNA assembly technologies. FEMS Yeast Res 2015;15(1):1–9. 链接1

[ 9 ] Quan J, Tian J. Circular polymerase extension cloning of complex gene libraries and pathways. PLoS One 2009;4(7):e6441. 链接1

[10] Li MZ, Elledge SJ. Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Nat Methods 2007;4(3):251–6. 链接1

[11] Cobb RE, Ning JC, Zhao H. DNA assembly techniques for next-generation combinatorial biosynthesis of natural products. J Ind Microbiol Biotechnol 2014;41(2):469–77. 链接1

[12] García-Nafría J, Watson JF, Greger IH. IVA cloning: a single-tube universal cloning system exploiting bacterial in vivo assembly. Sci Rep 2016;6(1):27459. 链接1

[13] Liang J, Liu Z, Low XZ, Ang EL, Zhao H. Twin-primer non-enzymatic DNA assembly: an efficient and accurate multi-part DNA assembly method. Nucleic Acids Res 2017;45(11):e94. 链接1

[14] Jin P, Ding W, Du G, Chen J, Kang Z. DATEL: a scarless and sequenceindependent DNA assembly method using thermostable exonucleases and ligase. ACS Synth Biol 2016;5(9):1028–32. 链接1

[15] Zhang Y, Werling U, Edelmann W. SLiCE: a novel bacterial cell extract-based DNA cloning method. Nucleic Acids Res 2012;40(8):e55. 链接1

[16] Engler C, Kandzia R, Marillonnet S. A one pot, one step, precision cloning method with high throughput capability. PLoS One 2008;3(11):e3647. 链接1

[17] Fu C, Donovan WP, Shikapwashya-Hasser O, Ye X, Cole RH. Hot fusion: an efficient method to clone multiple DNA fragments as well as inverted repeats without ligase. PLoS One 2014;9(12):e115318. 链接1

[18] Yoshida N, Sato M. Plasmid uptake by bacteria: a comparison of methods and efficiencies. Appl Microbiol Biotechnol 2009;83(5):791–8. 链接1

[19] Aune TEV, Aachmann FL. Methodologies to increase the transformation efficiencies and the range of bacteria that can be transformed. Appl Microbiol Biotechnol 2010;85(5):1301–13. 链接1

[20] Sleight SC, Bartley BA, Lieviant JA, Sauro HM. In-Fusion BioBrick assembly and re-engineering. Nucleic Acids Res 2010;38(8):2624–36. 链接1

[21] Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods 2009;6(5):343–5. 链接1

[22] Livak KJ, Schmittgen TD. Analysis of relative gene expression data using realtime quantitative PCR and the 2-DDCT method. Methods 2001;25(4): 402–8. 链接1

[23] De’ath G. Boosted trees for ecological modeling and prediction. Ecology 2007;88(1):243–51. 链接1

[24] SantaLucia J Jr, Hicks D. The thermodynamics of DNA structural motifs. Annu Rev Biophys Biomol Struct 2004;33(1):415–40. 链接1

[25] Lomzov AA, Vorobjev YN, Pyshnyi DV. Evaluation of the Gibbs free energy changes and melting temperatures of DNA/DNA duplexes using hybridization enthalpy calculated by molecular dynamics simulation. J Phys Chem B 2015;119(49):15221–34. 链接1

[26] Gao Y, Wolf LK, Georgiadis RM. Secondary structure effects on DNA hybridization kinetics: a solution versus surface comparison. Nucleic Acids Res 2006;34(11):3370–7. 链接1

[27] Lu YP, Zhang C, Lv FX, Bie XM, Lu ZX. Study on the electro-transformation conditions of improving transformation efficiency for Bacillus subtilis. Lett Appl Microbiol 2012;55(1):9–14. 链接1

[28] Tee KL, Grinham J, Othusitse AM, González-Villanueva M, Johnson AO, Wong TS. An efficient transformation method for the bioplastic-producing ‘‘Knallgas” bacterium Ralstonia eutropha H16. Biotechnol J 2017;12(11):1700081. 链接1

[29] Russo R, Panangala VS, Wood RR, Klesius PH. Chemical and electroporated transformation of Edwardsiella ictaluri using three different plasmids. FEMS Microbiol Lett 2009;298(1):105–10. 链接1

[30] Bzymek M, Lovett ST. Instability of repetitive DNA sequences: the role of replication in multiple mechanisms. Proc Natl Acad Sci USA 2001;98 (15):8319–25. 链接1

[31] Lovett ST. Encoded errors: mutations and rearrangements mediated by misalignment at repetitive DNA sequences. Mol Microbiol 2004;52 (5):1243–53. 链接1

[32] Young CL, Britton ZT, Robinson AS. Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications. Biotechnol J 2012;7(5):620–34. 链接1

[33] Pelicic V, Reyrat JM, Gicquel B. Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria. J Bacteriol 1996;178(4):1197–9. 链接1