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《工程(英文)》 >> 2023年 第21卷 第2期 doi: 10.1016/j.eng.2021.12.007

敲低特异性环状非编码RNA显著抑制骨肉瘤的进展

a Musculoskeletal Tumor Center, Peking University People’s Hospital, Beijing 100044, China
b Beijing Key Laboratory of Musculoskeletal Tumor, Peking University People’s Hospital,  Beijing 100044, China
c Department of Spine Surgery and Musculoskeletal Tumor, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
d State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
e Department of Chemistry, Tsinghua University, Beijing 100084, China

# These authors contributed equally to this work.

收稿日期: 2021-10-07 修回日期: 2021-11-24 录用日期: 2021-12-08 发布日期: 2022-01-04

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摘要

骨肉瘤是一种间充质组织来源的恶性肿瘤,好发于儿童和青少年,发生肺转移常导致患者死亡。关于骨肉瘤进展的机制仍不清楚。因此,迫切需要开发新的骨肉瘤治疗靶点和治疗模式。异常表达的非编码环状RNA(circRNA)对骨肉瘤的发生和发展至关重要。本研究的目的是探索一种新的circRNA circ_000203 在骨肉瘤中的表达和作用,阐明其潜在机制。我们发现,在骨肉瘤细胞系和组织中circ_000203 高表达,并且circ_000203 敲低显著抑制了体外和体内的骨肉瘤进展。此外,我们发现circ_000203 是miR-26b-5p 的海绵,而miR-26b-5p 是骨形态发生蛋白受体2(BMPR2)的上游调节因子。因此,BMPR2 的过表达可以减轻对骨肉瘤进展的抑制作用。这表明,敲低circ_000203 可通过miRNA介导的BMPR2 下调抑制骨肉瘤的进展。我们的发现为理解骨肉瘤的发生和发展提供了重要的见解。

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