智能手机成像的晶片上基于逆转录环介导等温扩增(RT-LAMP)技术的全血中HIV-1检测

2015年第1卷第3期

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《工程（英文）》 >> 2015年第1卷第3期 doi: 10.15302/J-ENG-2015072

智能手机成像的晶片上基于逆转录环介导等温扩增(RT-LAMP)技术的全血中HIV-1检测

¹ Department of Bioengineering, The University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
² Micro and Nanotechnology Laboratory, The University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
³ Department of Electrical and Computer Engineering, The University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

收稿日期： 2015-07-27 修回日期： 2015-08-26 录用日期： 2015-09-08 发布日期： 2015-09-30

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摘要

病毒载量测量对于人类免疫缺陷病毒 (HIV) 阳性患者长期临床护理来说是一个必不可少的工具。然而，考虑到病毒载量测量所需的仪器体积、成本和操作的复杂性，在医疗基础设施较差的偏远地区 (尤其是在被HIV感染人群比例较高的地区) 普及标准的病毒载量测量仪器是较为困难的。为提高该检测方法的普及性，人们已经开始开发可以进行即时检测的病毒载量检测平台，然而尚没有解决办法能够同时满足低成本、便携、易于操作等多种实际要求。本文通过运用微流体和微型硅晶片平台，对经过最低程度处理的含有HIV的全血样本进行了逆转录环介导等温扩增 (RT-LAMP)，并利用智能手机进行了荧光检测。集成实验检测结果表明，一滴约60 nL的反应液滴中仅有的3个病毒依然可以通过RT-LAMP技术被检测到，这相当于每微升全血样品中只有670个病毒。该技术在数字化RT-LAMP方法上具有重要意义，扩展该技术能够实现对HIV阳性患者在临床护理中采集指血进行病毒载量检测。研究结果显示，病毒载量检测过程所需的各个步骤，从血滴的准备到RT-LAMP反应的成像，都可以集成为晶片实验并且可以和移动设备兼容。

关键词

人类免疫缺陷病毒(HIV) ; 病毒载量 ; 环介导等温扩增 ; 智能手机 ; 即时检测

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