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2009, Volume 3, Issue 4

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Frontiers of Medicine >> 2009, Volume 3, Issue 4 doi: 10.1007/s11684-009-0087-5

Construction and humoral immune response of Epstein-Barr virus latent membrane protein 2 DNA vaccine in mice

1.Department of Internal Medicine, Tongji Hospital, Tongji Medical college, Huazhong University of Science and Technology, Wuhan 430030, China；Research Center for Neural Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, China; 2.Department of Internal Medicine, Tongji Hospital, Tongji Medical college, Huazhong University of Science and Technology, Wuhan 430030, China;

Available online: 2009-12-05
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Abstract

We constructed a eukaryotic expression plasmid encoding Epstein-Barr virus latent membrane protein 2 (EBV, LMP2) and evaluated its effects on humoral immunity. First, the encoding sequence of the EBV was amplified from B95−8 cell RNA by reverse transcription polymerase chain reaction (RT-PCR) and then was directionally cloned into eukaryotic expression vector pcDNA3.1. It was employed to evaluate immune response of the mice inoculated doubly with the DNA vaccine. The serum antibody against LMP2 was detected with enzyme-linked immunosorbent assay (ELISA). The recombinant plasmid pcDNA3.1- was confirmed by the restrictive endonuclease analysis and sequence analysis. The serum titer of IgG antibody against LMP2 epitope in the mice immunized with the DNA vaccine encoding LMP2 was up to 1∶4000. In conclusion, the EBV DNA vaccine can induce a strong humoral immune response in mice.

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