Journal Home Online First Current Issue Archive For Authors Journal Information 中文版

2015, Volume 9, Issue 3

Abstract

Keywords

Related Research

Back to Top

Frontiers of Chemical Science and Engineering >> 2015, Volume 9, Issue 3 doi: 10.1007/s11705-015-1525-3

High production of butyric acid by

Department of Chemical and Biological Engineering, The University of Alabama, 245 7th Avenue, Tuscaloosa, AL 35401, USA

Accepted: 2015-09-10 Available online: 2015-09-30
HTML10 PDF 2 Collect 0

Next Previous

Abstract

The objective of this study was to improve the production of butyric acid by process optimization using the metabolically engineered mutant of (PAK-Em). First, the free-cell fermentation at pH 6.0 produced butyric acid with concentration of 38.44 g/L and yield of 0.42 g/g. Second, the immobilized-cell fermentations using fibrous-bed bioreactor (FBB) were run at pHs of 5.0, 5.5, 6.0, 6.5 and 7.0 to optimize fermentation process and improve the butyric acid production. It was found that the highest titer of butyric acid, 63.02 g/L, was achieved at pH 6.5. Finally, the metabolic flux balance analysis was performed to investigate the carbon rebalance in . The results show both gene manipulation and fermentation pH change redistribute carbon between biomass, acetic acid and butyric acid. This study demonstrated that high butyric acid production could be obtained by integrating metabolic engineering and fermentation process optimization.

Related Research