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2010, Volume 4, Issue 4

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Frontiers of Environmental Science & Engineering >> 2010, Volume 4, Issue 4 doi: 10.1007/s11783-010-0250-x

Identification of cadmium-induced genes in maize seedlings by suppression subtractive hybridization

1. State Key Laboratory of Biocontrol, Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China; 2. College of Natural Resources, University of California, Berkeley, CA 94720, USA; 3. Department of Safety and Environmental Engineering, Hunan Institute of Technology, Hengyang 421002, China

Available online: 2010-12-05
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Abstract

A maize variety, Huatian-1, had an unusually low translocation rate of cadmium (Cd) (59.6 mg·kg in the roots and 0.093 mg·kg in the grain) compared to 24 other varieties while being grown in soils with 16.50 mg·kg Cd. This indicates that this particular species may have special mechanisms that affect the absorption and translocation pattern of Cd. In this paper, the technique of suppression subtractive hybridization (SSH) was used to isolate and identify Cd-induced genes from Huatian-1 hydroponically ?exposed? to? 0.1 mM ?CdCl ?for? 1 h,? 12 h, 24 h, and 48 h. We found a total of 15 differentially expressed genes in the four groups; 2, 3, 4, and 6 genes were from the groups of 1 h, 12 h, 24 h, and 48 h treatment, respectively. Phospholipase mRNA, adenosine triphosphate (ATP) phosphoribosyl transferase 2, and were turned on in the maize in response to Cd stress, and it might provide new clues to explain the mechanism of maize tolerance to Cd.

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