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Frontiers of Environmental Science & Engineering >> 2011, Volume 5, Issue 1 doi: 10.1007/s11783-010-0254-6

Community dynamics of ammonia oxidizing bacteria in a full-scale wastewater treatment system with nitrification stability

Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China

Available online: 2011-03-05

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Abstract

To determine whether the functional stability of nitrification was correlated to a stable community structure of ammonia oxidizing bacteria (AOB) in a full-scale wastewater treatment plant, the AOB community dynamics in a wastewater treatment system was monitored over one year. The community dynamics were investigated using specific PCR followed by terminal restriction fragment length polymorphism (T-RFLP) analysis of the gene. The T-RFLP results indicated that during the period of nitrification stability, the AOB community structure in the full-scale wastewater treatment system was relatively stable, and the average change rate every 15 d of the system was 6.6%±5.8%. The phylogenetic analysis of the cloned gene showed clearly that the dominant AOB in the system was spp. The results of this study indicated that throughout the study period, the AOB community structure was relatively stable in the full-scale wastewater treatment system with functional stability of nitrification.

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