Frontiers of Agricultural Science and Engineering
>> 2014,
Volume 1,
Issue 3
doi:
10.15302/J-FASE-2014020
LETTER
Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice
1. State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing100193, China.2. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650000, China
Accepted: 2014-12-03
Available online: 2015-01-27
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Abstract
Butyrylcholinesterase (BCHE) is a natural bioscavenger that protects humans against organophosphate toxicity. Due to the limited yield of human BCHE (hBCHE) when purifying from human plasma, it is necessary to find an alternative method to produce this protein. One potential method is to produce transgenic livestock that make modified milk containing high concentration of hBCHE. In this study, we cloned the gene into a human lactoferrin (hLF) bacterial artificial chromosome (BAC) construct to make a hLF-hBCHE BAC construct. Subsequently, we injected the BAC construct into pronuclei of mouse fertilized embryos and generated transgenic mice. Expression analysis showed that recombinant hBCHE (rhBCHE) was expressed efficiently in the mammary gland of the transgenic mice and the concentration of rhBCHE in the milk of individual mice ranged from 76±12 to 159±28 mg·L . Protein function tests showed that rhBCHE has the same enzymatic activity as the native hBCHE. Our results pave the way for making transgenic livestock to produce large quantities of rhBCHE.
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