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Frontiers of Agricultural Science and Engineering doi: 10.15302/J-FASE-2020374

FUNCTIONAL GAIN OF FRUIT NETTED-CRACKING IN AN INTROGRESSION LINE OF TOMATO WITH HIGHER EXPRESSION OF THE

Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan 430070, China

Received: 2020-12-31 Accepted: 2021-02-11 Available online: 2021-02-11

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Abstract

Fruit cracking is a major disorder that affects the integrity of fruit and reduces the commercial value of tomato and other fleshy fruit. Here, we have found a novel fruit ‘netted-cracking’ (FNC) phenotype in tomato introgression line IL4-4 which is present in neither the donor parent (LA0716) nor the receptor parent (M82). An F population was generated by crossing IL4-4 with M82 to genetically characterize the gene and this showed that a single dominant gene determined fruit netted-cracking. Further map-based cloning narrowed down the locus to a 230 kb region on chromosome 4. Sequencing and annotation analysis show that (Solyc04 g082540) was the most likely candidate gene. Functional characterization of by overexpressing and resulted in the fruit netted-cracking phenotype, suggesting that the transcript level results in the functional gain of fruit netted-cracking. These findings were further confirmed by ortholog in netted-cracking pepper and melon, indicating a common regulatory mechanism in different plant species. Furthermore, cytoplasm and nucleus-localized indicates increased expression of genes involved in suberin, lignin, lipid transport and cell wall metabolism. These findings provide novel genetic insights into fruit netted-cracking and offer a way to promote molecular improvement toward cracking resistant cultivars.

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