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Efficient acetoin production from pyruvate by engineered whole-cell biocatalysis

Frontiers of Chemical Science and Engineering   Pages 425-436 doi: 10.1007/s11705-022-2229-0

Abstract: Moreover, the reusability of the cell catalysis was also tested, and the result illustrated that thewhole-cell catalysis obtained 433.3, 440.2, 379.0, 442.8 and 339.4 mmol·L–1 (38.2, 38.8, 33.4This work therefore provided an efficient H. bluephagenesis whole-cell catalysis with a broad

Keywords： -acetolactate synthetase     α-acetolactate decarboxylase     Halomonas bluephagenesis     whole-cellbiocatalysis    

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Cell surface protein engineering for high-performance whole-cell catalysts

Hajime Nakatani,Katsutoshi Hori

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 46-57 doi: 10.1007/s11705-017-1609-3

Abstract: Cell surface protein engineering facilitated by accumulation of information on genome and protein structureinvolves heterologous production and modification of cell surface proteins using genetic engineering, and is important for the development of high-performance whole-cell catalysts., on the cell surface using a carrier protein.discuss advanced methods for displaying multiple enzymes and a new method for the immobilization of whole-cell

Keywords： cell surface engineering     surface display     whole-cell catalysts     bioprocess    

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Efficient production of D-1,2,4-butanetriol from D-xylose by engineered

Shewei Hu, Qian Gao, Xin Wang, Jianming Yang, Nana Xu, Kequan Chen, Sheng Xu, Pingkai Ouyang

Frontiers of Chemical Science and Engineering 2018, Volume 12, Issue 4,   Pages 772-779 doi: 10.1007/s11705-018-1731-x

Abstract: We have developed a whole-cell bioconversion system for the production of D-1,2,4-butanetriol (BT) fromA plasmid pETduet- T7- T7- was constructed and transformed to BL21(DE3) to obtain the whole cellsmetal ions, and culture conditions (pH, temperature, IPTG) were identified, and their effects on the whole-cellTo obtain the highest whole-cell activity, the optimal cultivation parameters are: 15 g·L yeast extractWith the optimized whole cells of BL21- , 60 g·L of xylose was converted to 28g·L BT with a molar

Keywords： D-1     2     4-butanetriol     whole-cell bioconversion     carbon source     nitrogen sources     metal ions     culture conditions    

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Organizational evolution of project management teams over the whole lifecycle of megaprojects: Case study

Frontiers of Engineering Management doi: 10.1007/s42524-022-0211-7

Abstract: Project management teams are critical in the implementation of megaprojects, but their evolution throughout the project lifecycle has not been clearly explained. This paper explores the organizational evolution of megaproject management teams through a longitudinal retrospective case study of the Hong Kong–Zhuhai–Macao Bridge (HZMB) project. The organizational evolution is examined in terms of management objectives, management content, and organizational structure. The organizational evolution of the HZMB project management team exhibits stage differentiation with the coexistence of turbulence and stability. Changes in the external environment are the driving force for organizational evolution, whereas a flexible organizational strategy is critical in promoting this evolution. Basing on the HZMB case study, this paper summarizes six critical measures that facilitate the organizational evolution of megaproject management teams. Our findings add value to megaproject management theory and provide a better understanding of the dynamics and complexity of megaproject organizational management.

Keywords： megaproject     organizational evolution     whole lifecycle management     project management team    

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TCGA whole-transcriptome sequencing data reveals significantly dysregulated genes and signaling pathways

Daniel Wai-Hung Ho,Alan Ka-Lun Kai,Irene Oi-Lin Ng

Frontiers of Medicine 2015, Volume 9, Issue 3,   Pages 322-330 doi: 10.1007/s11684-015-0408-9

Abstract:

This study systematically evaluates the TCGA whole-transcriptome sequencing data of hepatocellular

Keywords： TCGA     whole-transcriptome sequencing     HCC     liver cancer    

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Immobilization of

Xiaokai SONG,Zhongyi JIANG,Lin LI,Hong WU

Frontiers of Chemical Science and Engineering 2014, Volume 8, Issue 3,   Pages 353-361 doi: 10.1007/s11705-014-1421-2

Abstract: Mesoporous silica particles were prepared for efficient immobilization of the -glucuronidase (GUS) through a biomimetic mineralization process, in which the solution containing lysozyme and GUS were added into the prehydrolyzed tetraethoxysilane (TEOS) solution. The silica particles were formed in a way of biomineralization under the catalysis of lysozyme and GUS was immobilized into the silica particles simultaneously during the precipitation process. The average diameter of the silica particles is about 200 nm with a pore size of about 4 nm. All the enzyme molecules are tightly entrapped inside the biosilica nanoparticles without any leaching even under a high ionic strength condition. The immobilized GUS exhibits significantly higher thermal and pH stability as well as the storage and recycling stability compared with GUS in free form. No loss in the enzyme activity of the immobilized GUS was found after 30-day’s storage, and the initial activity could be well retained after 12 repeated cycles.

Keywords： storage and recycling stability     silica nanoparticles     biocatalysis     biomimetic synthesis     β-glucuronidase    

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Whole-life Thinking and Engineering the Future

Roger Flanagan

Frontiers of Engineering Management 2014, Volume 1, Issue 3,   Pages 290-296 doi: 10.15302/J-FEM-2014040

Abstract: Whole-life thinking for engineers working on the built environment has become more important in a fastWhole-life thinking is not new, every project attempts to balance the initial capital cost with the operatingSystems thinking provides one approach to developing a more robust whole-life approach.New approaches are needed to produce more reliable whole-life predictions.Best value, rather than lowest cost, can be achieved by using whole-life appraisal as a part of the design

Keywords： whole-life thinking     systems thinking     complexity     chaos     risk management through life    

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Innovations of phosphorus sustainability: implications for the whole chain

Jianbo SHEN, Liyang WANG, Xiaoqiang JIAO, Fanlei MENG, Lin ZHANG, Gu FENG, Junling ZHANG, Lixing YUAN, Lin MA, Yong HOU, Tao ZHANG, Weifeng ZHANG, Guohua LI, Kai ZHANG, Fusuo ZHANG

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 4,   Pages 321-331 doi: 10.15302/J-FASE-2019283

Abstract: The innovation and feasibility of P sustainability are highlighted from the perspective of the whole

Keywords： P-use efficiency     recycling     sustainable management     the whole P chain    

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Transforming bacterial disease surveillance and investigation using whole-genome sequence to probe the

Biao Kan, Haijian Zhou, Pengcheng Du, Wen Zhang, Xin Lu, Tian Qin, Jianguo Xu

Frontiers of Medicine 2018, Volume 12, Issue 1,   Pages 23-33 doi: 10.1007/s11684-017-0607-7

Abstract:

Two decades have passed since the first bacterial whole-genome sequencing, which provides new opportunityBacterial whole-genome sequencing and bioinformatic computing allow genotyping to satisfy the requirements

Keywords： genome sequencing     genomic epidemiology     bacteria     surveillance     infectious diseases    

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The Research on Subject Behavioral Risk of Whole Life-cycle Water Conservation Projects

Hong-yong Liu,Ji Guo,Wei-tao He

Frontiers of Engineering Management 2014, Volume 1, Issue 4,   Pages 348-352 doi: 10.15302/J-FEM-2014048

Abstract: This article takes each participating subject in the whole life-cycle of water conservation projects

Keywords： water conservation projects     subject behavior     safety accident     risk effect     engineering ethics    

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Accounting greenhouse gas emissions of food consumption between urban and rural residents in China: a whole

Frontiers in Energy 2022, Volume 16, Issue 2,   Pages 357-374 doi: 10.1007/s11708-021-0763-y

Abstract: Food consumption is necessary for human survival. On a global scale, the greenhouse gas (GHG) emission related to food consumption accounts for 19%–29% of the total GHG emission. China has the largest population in the world, which is experiencing a rapid development. Under the background of urbanization and the adjustment of the diet structure of Chinese residents, it is critical to mitigate the overall GHG emission caused by food consumption. This study aims to employ a single-region input-output (SRIO) model and a multi-regional input-output (MRIO) model to measure GHG emission generated from food consumption in China and compare the contributions of different industrial sectors, uncovering the differences between urban and rural residents and among different provinces (autonomous regions/municipalities), as well as identifying the driving forces of GHG emission from food consumption at a national level. The results indicate that the total GHG emission generated from food consumption in China tripled from 157 Mt CO2e in 2002 to 452 Mt CO2e in 2017. The fastest growing GHG emission is from the consumption of other processed food and meat products. Although GHG emissions from both urban and rural residents increased, the gap between them is increasing. Agriculture, processing and manufacture of food, manufacture of chemical and transportation, storage and post services sectors are key sectors inducing food consumption related GHG emissions. From a regional perspective, the top five emission provinces (autonomous regions/municipalities) include Shandong, Hubei, Guangdong, Zhejiang, and Jiangsu. Based on such results, policy recommendations are proposed to mitigate the overall GHG emission from food consumption.

Keywords： greenhouse gas (GHG) emission     food consumption     industry sectors     mitigation measures     urban governance    

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Simultaneous acquisition of CT angiography and whole brain CT perfusion images by using multiphase dynamic

Weiwei CHEN, Jianpin QI, Wenzhen ZHU, Wenhua HUANG, Jinmei SONG

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 230-235 doi: 10.1007/s11684-009-0023-8

Abstract: We implemented a new protocol — multiphase dynamic helical scan to acquire CT angiography (CTA) and whole

Keywords： computed tomography angiography     computed tomography perfusion    

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Exome sequencing greatly expedites the progressive research of Mendelian diseases

Xuejun Zhang

Frontiers of Medicine 2014, Volume 8, Issue 1,   Pages 42-57 doi: 10.1007/s11684-014-0303-9

Abstract:

The advent of whole-exome sequencing (WES) has facilitated the discovery of rare structure and functional

Keywords： genetics     whole-exome sequencing     Mendelian disease     disease gene    

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Comments on “Innovations for phosphorus sustainability: implications for the whole food chain” in special

Peter M. VITOUSEK, Xuejun LIU

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 4,   Pages 441-442 doi: 10.15302/J-FASE-2019284

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Extracts from cotton over the whole growing season induce

Ming LANG, Rui YU, Yongqing MA, Wei ZHANG, Christopher S. P. McErlean

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 2,   Pages 228-236 doi: 10.15302/J-FASE-2017150

Abstract: Extracts of cotton across the whole growing season were able to induce germination but displayed significant

Keywords： agricultural systems     cotton     crop ecology     crop rotation systems     weed management    

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Title Author Date Type Operation

Efficient acetoin production from pyruvate by engineered whole-cell biocatalysis

Journal Article

Cell surface protein engineering for high-performance whole-cell catalysts

Hajime Nakatani,Katsutoshi Hori

Journal Article

Efficient production of D-1,2,4-butanetriol from D-xylose by engineered

Shewei Hu, Qian Gao, Xin Wang, Jianming Yang, Nana Xu, Kequan Chen, Sheng Xu, Pingkai Ouyang

Journal Article

Organizational evolution of project management teams over the whole lifecycle of megaprojects: Case study

Journal Article

TCGA whole-transcriptome sequencing data reveals significantly dysregulated genes and signaling pathways

Daniel Wai-Hung Ho,Alan Ka-Lun Kai,Irene Oi-Lin Ng

Journal Article

Immobilization of

Xiaokai SONG,Zhongyi JIANG,Lin LI,Hong WU

Journal Article

Whole-life Thinking and Engineering the Future

Roger Flanagan

Journal Article

Innovations of phosphorus sustainability: implications for the whole chain

Jianbo SHEN, Liyang WANG, Xiaoqiang JIAO, Fanlei MENG, Lin ZHANG, Gu FENG, Junling ZHANG, Lixing YUAN, Lin MA, Yong HOU, Tao ZHANG, Weifeng ZHANG, Guohua LI, Kai ZHANG, Fusuo ZHANG

Journal Article

Transforming bacterial disease surveillance and investigation using whole-genome sequence to probe the

Biao Kan, Haijian Zhou, Pengcheng Du, Wen Zhang, Xin Lu, Tian Qin, Jianguo Xu

Journal Article

The Research on Subject Behavioral Risk of Whole Life-cycle Water Conservation Projects

Hong-yong Liu,Ji Guo,Wei-tao He

Journal Article

Accounting greenhouse gas emissions of food consumption between urban and rural residents in China: a whole

Journal Article

Simultaneous acquisition of CT angiography and whole brain CT perfusion images by using multiphase dynamic

Weiwei CHEN, Jianpin QI, Wenzhen ZHU, Wenhua HUANG, Jinmei SONG

Journal Article

Exome sequencing greatly expedites the progressive research of Mendelian diseases

Xuejun Zhang

Journal Article

Comments on “Innovations for phosphorus sustainability: implications for the whole food chain” in special

Peter M. VITOUSEK, Xuejun LIU

Journal Article

Extracts from cotton over the whole growing season induce

Ming LANG, Rui YU, Yongqing MA, Wei ZHANG, Christopher S. P. McErlean

Journal Article