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Manihot esculenta 1

Musa acuminata 1

Musa balbisiana 1

Green Revolution 1

banana (Musa spp) 1

cold stress 1

microRNA 1

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sub-Saharan Africa 1

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Sustainable intensification of agriculture in Africa

Antonius G.T. SCHUT, Ken E. GILLER

Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 4, Pages 371-375 doi: 10.15302/J-FASE-2020357

Abstract: Sustainable intensification is a key component of agricultural development in Africa, urgently needed to wean the continent off foreign food supply and to limit agricultural farmland expansion. It is expected that a relatively small fraction of farmers will adopt fertilizer technology, as profits in current economic settings are relatively small while risks are considerable with varying prices and uncertain yield responses. Many smallholders depend on off-farm income and local markets for food supply. Structural adjustments are therefore needed to allow management of larger units of land by trained farmers willing to take this opportunity, while recognizing land right sensitivities. There are large opportunities for African commodity crops to improve food security, including cassava and East African highland banana that strongly respond to fertilizer with limited environmental risks under good management. This requires investments in better functioning markets, local fertilizer production facilities that can produce regional crop blends and cost-efficient distribution networks, providing balanced fertilizers for African farmers.

Keywords： Green Revolution Manihot esculenta Musa acuminata sub-Saharan Africa

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Cold stress responsive microRNAs and their targets in

Jingyi WANG,Juhua LIU,Caihong JIA,Hongxia MIAO,Jianbin ZHANG,Zhuo WANG,Biyu XU,Zhiqiang JIN

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 4, Pages 335-345 doi: 10.15302/J-FASE-2016121

Abstract: Cold stress is an environmental factor affecting plant development and production. Recently, microRNAs (miRNAs) have been found to be involved in several plant processes such as growth regulation and stress responses. Although miRNAs and their targets have been identified in several banana species, their participation during cold accumulation in banana remains unknown. In this study, two small RNA libraries were generated from micropropagated plantlets of grown at normal and low temperature (5°C). A total of 69 known miRNAs and 32 putative novel miRNAs were detected in the libraries by Solexa sequencing. Sixty-four cold-inducible miRNAs were identified through differentially expressed miRNAs analysis. Among 43 miRNAs belonging to 26 conserved miRNA families with altered expression, 18 were upregulated and 25 downregulated under cold stress. Of 21 putative novel miRNAs with altered expression, four were downregulated and 17 upregulated. Furthermore, eight miRNAs were validated by stem-loop qRT-PCR and their dynamic differential expression was analyzed. In addition, 393 target genes of 58 identified cold-responsive miRNAs were predicted and categorized by function. These results provide important information for further characterization and functional analysis of cold-responsive miRNAs in banana.

Keywords： cold stress microRNA Musa balbisiana

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Efficient regeneration system applicable to five

Juhua LIU,Peiguang SUN,Jing ZHANG,Jiashui WANG,Jianbin ZHANG,Jingyi WANG,Caihong JIA,Pengzhao GAO,Biyu XU,Zhiqiang JIN

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 4, Pages 330-334 doi: 10.15302/J-FASE-2016118

Abstract: Banana ( spp.) is an important staple food, economic crop, and nutritional fruit worldwide. Hybridization is seriously hampered by the long generation time, polyploidy, and sterility of most cultivars. Establishment of an efficient regeneration and transformation system for banana is critical for their genetic improvement. An efficient and reproducible transformation system for banana using direct organogenesis was developed. Media containing benzylaminopurine (BA) combined with one of four other growth regulators was evaluated for the regeneration efficiency of five cultivars and the ability to induce/support development of new banana shoots. The result indicated that the greatest number of shoots per explant for all five cultivars was obtained using MS medium supplemented with 8.9 mmol·L BA and 9.1 mmol·L thidiazuron (TDZ). In 240–270 d, one immature male flower could regenerate between 380 and 456, 310–372, 200–240, 130–156, and 100–130 well-developed shoots for Gongjiao, Red banana, Rose banana, Baxi, and Xinglongnaijiao, respectively. Such a system will facilitate molecular breeding and functional genomics of banana.

Keywords： banana (Musa spp) system regeneration