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Journal Article 6

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2019 1

2018 1

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Saccharomyces cerevisiae 2

Saccharomyces cerevisiae 2

synthetic biology 2

β-carotene hydroxylase 1

β-carotene ketolase 1

Saccharomyces cerevisiae (S. cerevisiae) 1

saccharomyces cerevisiae 1

Caffeic acid 1

Cr(III)-organic complexes 1

Cytochrome P450 1

EC50 1

Heterologous enzyme 1

SCRaMbLE 1

Synthetic biology 1

astaxanthin 1

bioavailability 1

biorefinery engineering 1

biosorption 1

desorption 1

genome rearrangement 1

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Exposure-response of Cr(III)-organic complexes to Saccharomyces cerevisiae

Nivedita CHATTERJEE, Zejiao LUO,

Frontiers of Environmental Science & Engineering 2010, Volume 4, Issue 2,   Pages 196-202 doi: 10.1007/s11783-010-0008-5

Abstract: Hexavalent chromium [Cr(VI)] bioreduction produces soluble Cr(III)-organic complexes. The Cr(III)-organic complexes are relatively stable once they are formed, and no data about their toxicity were reported. Therefore, this study aims to investigate the bioavailability and toxicity of the soluble Cr(III)-organic complexes. L-1 wild type yeast strain was chosen as the model organism and Cr(III)-citrate was selected as the representative compound of the Cr(III)-organic complexes. The short-term chronic aquatic toxicity tests of the Cr(III)-citrate was explored by measuring growth inhibition, direct viable cell count, dry biomass, biosorption, and the amount of CO production. Cr(III)-citrate exerted a toxicity of 51mg/L with an , which was calculated from the percent growth inhibition. These toxicity data would be helpful to define the toxic potential of the organo-chromium-III compounds in the environment.

Keywords： Cr(III)-organic complexes     Saccharomyces cerevisiae     toxicity     EC50     bioavailability    

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Biosorption of Cd

Fengyu ZAN, Shouliang HUO, Beidou XI, Xiulan ZHAO

Frontiers of Environmental Science & Engineering 2012, Volume 6, Issue 1,   Pages 51-58 doi: 10.1007/s11783-011-0206-9

Abstract: The biosorption of Cd and Cu onto the immobilized ( ) was investigated in this study. Adsorption kinetics, isotherms and the effect of pH were studied. The results indicated that the biosorption of Cd and Cu on the immobilized was fast at initial stage and then became slow. The maximum biosorption of heavy metal ions on immobilized were observed at pH 4 for Cd and Cu . by the pseudo-second-order model described the sorption kinetic data well according to the high correlation coefficient ( ) obtained. The biosorption isotherm was fitted well by the Langmuir model, indicating possible mono-layer biosorption of Cd and Cu on the immobilized . Moreover, the immobilized after the sorption of Cd and Cu could be regenerated and reused.

Keywords： Saccharomyces cerevisiae (S. cerevisiae)     biosorption     heavy metals     immobilization    

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Engineering the Biosynthesis of Caffeic Acid in Saccharomyces cerevisiae with Heterologous Enzyme Combinations Article

Lanqing Liu, Hong Liu, Wei Zhang, Mingdong Yao, Bingzhi Li, Duo Liu, Yingjin Yuan

Engineering 2019, Volume 5, Issue 2,   Pages 287-295 doi: 10.1016/j.eng.2018.11.029

Abstract: mdash;from several bacteria, we constructed functional 4-hydroxyphenylacetate 3-hydroxylase (4HPA3H) in Saccharomyces

Keywords： Saccharomyces cerevisiae     Caffeic acid     Heterologous enzyme     Cytochrome P450     Synthetic biology    

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Comparative lipidomic analysis of

Bin QIAO, Hong-Chi TIAN, Ying-Jin YUAN

Frontiers of Chemical Science and Engineering 2012, Volume 6, Issue 4,   Pages 461-469 doi: 10.1007/s11705-012-1223-3

Abstract: Variations in the composition and level of phospholipids (PLs) in yeast cells during industrial ethanol fermentation processes were analyzed. A comparative lipidomic method was used to investigate the changes in total cellular PLs during continuous and fed-batch/batch processes. The phospholipid metabolism in yeast changed during both processes, mainly due to the presence of long-chain poly unsaturated fatty acids (PUFA) that contained phosphatidyglycerol (PG), phosphatidylethanolamine (PE) and phosphatidylserine (PS). The complexity of the media affected the growth of the yeast and the membrane composition. Yeast incorporated lots of exogenous saturated and PUFAs from the feedstock during the fermentations. During the continuous fermentation, there was an increase in PLs with shorter chains as the fermentation progressed and early in process there were more long-chains. During the fed-batch/batch process, the PG species increased as the fermentation progressed. This is probably due to an inositol deficiency in the earlier part of the fermentation.

Keywords： lipidomics     systems biology     phospholipids     stirred-tank     saccharomyces cerevisiae     biorefinery    

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Improving prodeoxyviolacein production via multiplex SCRaMbLE iterative cycles

Juan Wang, Bin Jia, Zexiong Xie, Yunxiang Li, Yingjin Yuan

Frontiers of Chemical Science and Engineering 2018, Volume 12, Issue 4,   Pages 806-814 doi: 10.1007/s11705-018-1739-2

Abstract:

The synthetic chromosome rearrangement and modification by loxP-mediated evolution (SCRaMbLE) system has been used to improve prodeoxyviolacein (PDV) production in haploid yeast containing chromosome synV. To rapidly and continuously generate genome diversification with the desired phenotype, the multiplex SCRaMbLE iterative cycle strategy has been developed for the screening of high PDV production strains. Whole-genome sequencing analysis reveals large duplications, deletions, and even the whole genome duplications. The deletion of YER151C is proved to be responsible for the increase. This study demonstrates that artificial DNA rearrangement can be used to accelerate microbial evolution and the production of biobased chemicals.

Keywords： synthetic biology     genome rearrangement     prodeoxyviolacein     SCRaMbLE     Saccharomyces cerevisiae    

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Engineering of

Ruizhao Wang,Xiaoli Gu,Mingdong Yao,Caihui Pan,Hong Liu,Wenhai Xiao,Ying Wang,Yingjin Yuan

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 89-99 doi: 10.1007/s11705-017-1628-0

Abstract: The conversion of -carotene to astaxanthin is a complex pathway network, in which two steps of hydroxylation and two steps of ketolation are catalyzed by -carotene hydroxylase (CrtZ) and -carotene ketolase (CrtW) respectively. Here, astaxanthin biosynthesis pathway was constructed in by introducing heterologous CrtZ and CrtW into an existing high -carotene producing strain. Both genes and were codon optimized and expressed under the control of constitutive promoters. Through combinatorial expression of CrtZ and CrtW from diverse species, nine strains in dark red were visually chosen from thirty combinations. In all the selected strains, strain SyBE_Sc118060 with CrtW from DC263 and CrtZ from sp. strain PC-1 achieved the highest astaxanthin yield of 3.1 mg/g DCW. Protein phylogenetic analysis shows that the shorter evolutionary distance of CrtW is, the higher astaxanthin titer is. Further, when the promoter of in strain SyBE_Sc118060 was replaced from FBA1p to TEF1p, the astaxanthin yield was increased by 30.4% (from 3.4 to 4.5 mg/g DCW). In the meanwhile, 33.5-fold increase on transcription level and 39.1-fold enhancement on the transcriptional ratio of to were observed at early exponential phase in medium with 4% (w/v) glucose. Otherwise, although the ratio of to were increased at mid-, late-exponential phases in medium with 2% (w/v) glucose, the transcription level of both and were actually decreased during the whole time course, consequently leading to no significant improvement on astaxanthin production. Finally, through high cell density fed-batch fermentation using a carbon source restriction strategy, the production of astaxanthin in a 5-L bioreactor reached to 81.0 mg/L, which was the highest astaxanthin titer reported in yeast. This study provides a reference to greatly enhance desired compounds accumulation by employing the key enzyme(s) in microbes.

Keywords： synthetic biology     astaxanthin     β-carotene hydroxylase     β-carotene ketolase     Saccharomyces    

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Title Author Date Type Operation

Exposure-response of Cr(III)-organic complexes to Saccharomyces cerevisiae

Nivedita CHATTERJEE, Zejiao LUO,

Journal Article

Biosorption of Cd

Fengyu ZAN, Shouliang HUO, Beidou XI, Xiulan ZHAO

Journal Article

Engineering the Biosynthesis of Caffeic Acid in Saccharomyces cerevisiae with Heterologous Enzyme Combinations

Lanqing Liu, Hong Liu, Wei Zhang, Mingdong Yao, Bingzhi Li, Duo Liu, Yingjin Yuan

Journal Article

Comparative lipidomic analysis of

Bin QIAO, Hong-Chi TIAN, Ying-Jin YUAN

Journal Article

Improving prodeoxyviolacein production via multiplex SCRaMbLE iterative cycles

Juan Wang, Bin Jia, Zexiong Xie, Yunxiang Li, Yingjin Yuan

Journal Article

Engineering of

Ruizhao Wang,Xiaoli Gu,Mingdong Yao,Caihui Pan,Hong Liu,Wenhai Xiao,Ying Wang,Yingjin Yuan

Journal Article