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2019 1

2013 1

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Escherichia coli 1

Aminoglycoside resistance plasmids 1

Characteristics 1

Persistence 1

Transfer 1

aminoglycoside 1

high-performance liquid chromatography 1

ion-pairing 1

refractive index detector 1

tobramycin 1

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Characteristics of two transferable aminoglycoside resistance plasmids in

Chengjun Pu, Xiaoyan Gong, Ying Sun

Frontiers of Environmental Science & Engineering 2019, Volume 13, Issue 3, doi: 10.1007/s11783-019-1119-2

Abstract:

pRKZ3 is a non-conjugative IncQ plasmid, while pKANJ7 is a conjugative IncX plasmid.

The optimal mating time of pKANJ7 varied under different conditions.

Both of the two transferable ARPs had little impact on the growth of their hosts.

A relatively high level of fitness cost was observed for pKANJ7.

The fitness cost of ARPs depended on their hosts.

Keywords： Escherichia coli Characteristics Aminoglycoside resistance plasmids Transfer Persistence

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Fast determination of tobramycin by reversed-phase ion-pair high performance liquid chromatography with a refractive index detector

Liang ZHU, Jingkang WANG

Frontiers of Chemical Science and Engineering 2013, Volume 7, Issue 3, Pages 322-328 doi: 10.1007/s11705-013-1348-z

Abstract: A simple and direct method without a derivation step for routine analysis of tobramycin has been developed. This method used reversed-phase ion-pair high performance liquid chromatography (HPLC) with a refractive index (RI) detector and a C18 column which is stable at pH above 1.00. The presence of 4.50 mg·mL trifluoroacetic acid (TFA) in the mobile phase improved the protonation of tobramycin and the formation of ion-pairs, and thus reduced its hydrophility. This unique separation–detection combination showed good linearity with correlation coefficients 0.9996 in the concentration range of 0.25–2.50 mg·mL . The quantitation limit and detection limit were determined to be 0.23 mg·mL and 0.071 mg·mL , respectively. Tobramycin was recovered in 98.00%, 98.84% and 99.64% for tobramycin solutions at concentrations of 2.25 mg·mL , 1.50 mg·mL and 0.75 mg·mL , respectively. The relative standard deviations for six spiked samples ranged from 0.20% to 2.40%, indicating a good reproducibility of this method.

Keywords： tobramycin aminoglycoside refractive index detector high-performance liquid chromatography ion-pairing