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advance bioprocess development and biomanufacturing 1

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Advanced Bioprocess Development and Manufacturing Technologies in High-Throughput Miniature Bioreactors

Jiao Peng, Chen Biqiang

Strategic Study of CAE 2016, Volume 18, Issue 4, Pages 44-50 doi: 10.15302/J-SSCAE-2016.04.007

Abstract: However, the traditional technologies for bioprocess development and manufacturing will not succeed inand Service, proposed and started working on the next generation of technology platforms for advanced bioprocessdevelopment and manufacturing (ABDM).The development of ABDM is to significantly reduce the timeframe for bioprocessthis platform will radiate to the entire biomanufacture industry, and open a whole new era for the bioprocess

Keywords： advance bioprocess development and biomanufacturing virulent infectious disease precision medicine

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Cell surface protein engineering for high-performance whole-cell catalysts

Hajime Nakatani,Katsutoshi Hori

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1, Pages 46-57 doi: 10.1007/s11705-017-1609-3

Abstract: Cell surface protein engineering facilitated by accumulation of information on genome and protein structure involves heterologous production and modification of cell surface proteins using genetic engineering, and is important for the development of high-performance whole-cell catalysts. In this field, cell surface display is a major technology by exposing target proteins, such as enzymes, on the cell surface using a carrier protein. The target proteins are fused to the carrier proteins that transport and tether them to the cell surface, as well as to a secretion signal. This paper reviews cell surface display systems for prokaryotic and eukaryotic cells from the perspective of carrier proteins, which determine the number of displayed molecules, and the localization, size, and direction ( or terminal anchoring) of the passengers. We also discuss advanced methods for displaying multiple enzymes and a new method for the immobilization of whole-cell catalysts using adhesive surface proteins.

Keywords： cell surface engineering surface display whole-cell catalysts bioprocess