Resource Type

Journal Article 9

Year

2023 2

2022 2

2021 1

2019 1

2017 2

Keywords

3-hydroxyvalerate (3HV) 1

Brucella melitensis 1

S. cerevisiae 1

Caffeic acid 1

Cell death 1

Cyclic lipopeptide 1

Cytochrome P450 1

Dual-antigen 1

Heterologous enzyme 1

Leucine-rich repeat extension (LRX) 1

MdMYB1 1

Medpeptin 1

Mixed culture 1

Nanoconjugate vaccine 1

Polyhydroxyalkanoates (PHAs) 1

Propionate 1

Protein glycan coupling technology (PGCT) 1

Pseudomonas 1

Receptor-like kinase (RLK) 1

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THE ANTHOCYANIN BIOSYNTHETIC REGULATOR MDMYB1 POSITIVELY REGULATES ASCORBIC ACID BIOSYNTHESIS IN APPLE

Frontiers of Agricultural Science and Engineering 2021, Volume 8, Issue 2,

Abstract:

Ascorbic acid (AsA, vitamin C) is involved in the regulation of many aspects of plant growth and development. It is an essential micronutrient for humans and can prevent scurvy, maintain the health of gums and blood vessels, reduce the level of plasma cholesterol and enhance the immune systen. Apple cultivars Orin and Guanghui were crossed to obtain a group of hybrid offspring with and without red flesh in the course of assessing apple germplasm resources. Unexpectedly, the red-flesh apples had higher AsA contents than other apples. Further studies showed that the anthocyanin biosynthetic regulator MdMYB1 directly activates the expression of dehydroascorbate reductase gene MdDHAR, thus promoting the activity of the DHAR enzyme and the accumulation of AsA. This finding reveals the mechanism leading to high AsA levels in red-flesh apples and suggests a new idea to cultivate red-flesh apples with high AsA contents and produce AsA efficiently and without pollution.

 

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Development of a dual temperature control system for isoprene biosynthesis in

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 7,   Pages 1079-1089 doi: 10.1007/s11705-021-2088-0

Abstract: Conflict between cell growth and product accumulation is frequently encountered in the biosynthesis ofstrains harboring the isoprene synthetic pathway in the mitochondria, the dynamic control of isoprene biosynthesisprovides a dual temperature control system for yeast engineering that may also be conducive to the biosynthesis

Keywords： transcriptional activator     directed evolution     dynamic control     heat-shock     isoprene    

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THE ANTHOCYANIN BIOSYNTHETIC REGULATOR MDMYB1 POSITIVELY REGULATES ASCORBIC ACID BIOSYNTHESIS IN APPLE

Jianping AN, Xiaofei WANG, Chunxiang YOU, Yujin HAO

Frontiers of Agricultural Science and Engineering   Pages 231-235 doi: 10.15302/J-FASE-2020367

Abstract: Ascorbic acid (AsA, vitamin C) is involved in the regulation of many aspects of plant growth and development. It is an essential micronutrient for humans and can prevent scurvy, maintain the health of gums and blood vessels, reduce the level of plasma cholesterol and enhance the immune systen. Apple cultivars Orin and Guanghui were crossed to obtain a group of hybrid offspring with and without red flesh in the course of assessing apple germplasm resources. Unexpectedly, the red-flesh apples had higher AsA contents than other apples. Further studies showed that the anthocyanin biosynthetic regulator MdMYB1 directly activates the expression of dehydroascorbate reductase gene , thus promoting the activity of the DHAR enzyme and the accumulation of AsA. This finding reveals the mechanism leading to high AsA levels in red-flesh apples and suggests a new idea to cultivate red-flesh apples with high AsA contents and produce AsA efficiently and without pollution.

Keywords： anthocyanin     apples     ascorbic acid     MdMYB1     vitamin C    

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Investigation of polyhydroxyalkanoates (PHAs) biosynthesis from mixed culture enriched by valerate-dominant

Jiuxiao Hao, Xiujin Wang, Hui Wang

Frontiers of Environmental Science & Engineering 2017, Volume 11, Issue 1, doi: 10.1007/s11783-017-0896-8

Abstract: Valerate-hydrolysate enriched culture showed great ability to produce 3HV and 3H2MV. Valerate-hydrolysate enriched culture had more in the community. Mixed iso-/n-valerate was effective at balancing microbial growth and PHAs synthesis. Co-substrates of valerate and propionate raised the fraction of 3HV and 3H2MV. The production of polyhydroxyalkanoates (PHAs) with a high fraction of 3-hydroxyvalerate (3HV) and 3-hydroxy-2-methylvalerate (3H2MV) from mixed culture enriched by valerate-dominant hydrolysate was evaluated in this study. After long-term enrichment, the culture showed strong ability to synthesize 3HV and 3H2MV, even with acetate-dominant substrate. The ultilization of single or mixed iso-/n-valerate by the enriched culture showed that the mixture of iso-valerate and n-valerate was more efficient substrate than any single in terms of balancing microbial growth and PHAs synthesis. Besides, through comparing the kinetics and stoichiometry of the tests supplying valerate and propionate, the enriched culture with equivalent valerate and propionate (1:1 molar ratio) exhibited superior PHAs production performances to pure valerate or propionate, attaining more than 70 mol% of 3HV and 3H2MV. The above findings reveal that valerate-dominant hydrolysate is a kind of suitable substrate to enrich PHAs producing culture with great capability to synthesize 3HV and 3H2MV monomers, thus improving product properties than pure poly(3-hydroxybutyrate) (P3HB); also 3HV and 3H2MV production behaviors can be regulated by the type of odd-carbon VFAs in the substrate.

Keywords： Polyhydroxyalkanoates (PHAs)     Valerate     Mixed culture     3-hydroxyvalerate (3HV)     Propionate    

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Assembly of biosynthetic pathways in

Lidan Ye,Xiaomei Lv,Hongwei Yu

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 126-132 doi: 10.1007/s11705-016-1597-8

Abstract: A robust and versatile tool for multigene pathway assembly is a key to the biosynthesis of high-valueFunctional isoprenoid biosynthesis pathways containing 4–11 genes with lengths ranging from ~10 to ~22

Keywords： pathway assembly     toolbox     reiterative recombination     S. cerevisiae     biosynthesis    

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PRODUCTION OF NEW WAP-8294A CYCLODEPSIPEPTIDES BY THE BIOLOGICAL CONTROL AGENT LYSOBACTER ENZYMOGENES OH11

Frontiers of Agricultural Science and Engineering 2022, Volume 9, Issue 1,   Pages 120-132 doi: 10.15302/J-FASE-2021410

Abstract:

Naturally-occurring environmental microorganisms may provide ‘green’ and effective biocontrol tools for disease management in agricultural crops. Due to the constant threat of resistant pathogens there is a pressing and continual need to search for new biocontrol tools. This study investigated the production of new analogs of WAP-8294A compounds by the biocontrol agent Lysobacter enzymogenes OH11 through biosynthetic engineering. WAP-8294As are a family of natural cyclic lipodepsipeptides with potent activity against Gram-positive bacteria. A series of genetic manipulations was therefore conducted on the accessory genes in the WAP biosynthetic gene cluster. The resulting strains containing a single-point mutation in ORF4, which was predicted to encode a 2-ketoglutarate dependent dioxygenase, produced deoxy-WAP-8294As. This result provides evidence for the function of ORF4 in catalyzing β-hydroxylation of the D-asparagine residue in WAP-8294As. In addition, six new analogs of WAP-8294As were identified by UHPLC-HR-MS/MS. This is the first attempt to produce new WAP-8294As in Lysobacter and shows that the spectrum of the biocontrol compounds may be expanded through the manipulation of biosynthetic genes.

 

Keywords： biocontrol / biosynthesis / Lysobacter / natural products / WAP-8294A    

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Engineering the Biosynthesis of Caffeic Acid in Saccharomyces cerevisiae with Heterologous Enzyme Combinations Article

Lanqing Liu, Hong Liu, Wei Zhang, Mingdong Yao, Bingzhi Li, Duo Liu, Yingjin Yuan

Engineering 2019, Volume 5, Issue 2,   Pages 287-295 doi: 10.1016/j.eng.2018.11.029

Abstract:

Engineering the biosynthesis of plant-derived natural products in microbes presents several challenges

Keywords： Saccharomyces cerevisiae     Caffeic acid     Heterologous enzyme     Cytochrome P450     Synthetic biology    

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Pseudomonas Cyclic Lipopeptide Medpeptin: Biosynthesis and Modulation of Plant Immunity Article

Yi-Lin Gu, Jun-Zhou Li, Yan Li, Shen Cong, Jing Wang, Yi-Nan Ma, Hai-Lei Wei

Engineering 2023, Volume 28, Issue 9,   Pages 153-165 doi: 10.1016/j.eng.2023.05.016

Abstract:

The multifunctional secondary metabolites known as cyclic lipopeptides (CLPs), which are produced by a large variety of bacteria, have become a key category of plant immunity elicitors. Pseudomonas-CLPs (Ps-CLPs) are extremely diverse in structure and biological activity. However, an understanding of CLP-plant structure–function interactions currently remains elusive. Here, we identify medpeptin, a novel CLP from Pseudomonas mediterranea that consists of 22 amino acids. Medpeptin is synthesized by a non-ribosomal peptide synthase (NRPS) gene cluster and regulated by a quorum-sensing system. Further research indicates that medpeptin does not exhibit antimicrobial activity; instead, it induces plant cell death immunity and confers resistance to bacterial infection. Comparative transcriptome analysis and virus-induced gene silencing (VIGS) reveal a set of immune signaling candidates involved in medpeptin perception. Silencing of a cell-wall leucine-rich repeat extensin protein (NbLRX3) or a receptor-like protein kinase (NbRLK25)—but not BAK1 or SGT1—compromises medpeptin-triggered cell death and resistance to pathogen infection in Nicotiana benthamiana. Our findings point to a non-canonical mechanism of CLP sensing and suggest perspectives for the development of plant disease resistance.

 

Keywords： Pseudomonas     Cyclic lipopeptide     Cell death     Leucine-rich repeat extension (LRX)     Medpeptin     Receptor-like kinase (RLK)    

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Biosynthesis and Immunological Evaluation of a Dual-Antigen Nanoconjugate Vaccine against Brucella Article

Jing Huang, Yufei Wang, Kangfeng Wang, Shulei Li, Peng Sun, Yan Guo, Jiankai Liu, Ruifu Yang, Ming Zeng, Chao Pan, Hengliang Wang, Li Zhu

Engineering 2023, Volume 29, Issue 10,   Pages 95-109 doi: 10.1016/j.eng.2023.04.007

Abstract:

Brucellosis, caused by Brucella, is one of the most common zoonosis. However, there is still no vaccine for human use. Although some live attenuated vaccines have been approved for animals, the protection effect is not ideal. In this study, we developed a dual-antigen nanoconjugate vaccine containing both polysaccharide and protein antigens against Brucella. First, the antigenic polysaccharide was covalently coupled to the outer membrane protein Omp19 using protein glycan coupling technology, and then it was successfully loaded on a nano-carrier through the SpyTag/SpyCatcher system. After confirming the efficient immune activation and safety performance of the dual-antigen nanoconjugate vaccine, the potent serum antibody response against the two antigens and remarkable protective effect in nonlethal
and lethal Brucella infection models were further demonstrated through different routes of administration. These results indicated that the dual-antigen nanoconjugate vaccine enhanced both T helper 1 cell (Th1) and Th2 immune responses and protected mice from Brucella infection. Furthermore, we found that this protective effect was maintained for at least 18 weeks. To our knowledge, this is the first Brucella
vaccine bearing diverse antigens, including a protein and polysaccharide, on a single nanoparticle. Thus, we also present an attractive technology for co-delivery of different types of antigens using a strategy applicable to other vaccines against infectious diseases.

Keywords： Protein glycan coupling technology (PGCT)     Dual-antigen     Nanoconjugate vaccine     Brucella melitensis    

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Title Author Date Type Operation

THE ANTHOCYANIN BIOSYNTHETIC REGULATOR MDMYB1 POSITIVELY REGULATES ASCORBIC ACID BIOSYNTHESIS IN APPLE

Journal Article

Development of a dual temperature control system for isoprene biosynthesis in

Journal Article

THE ANTHOCYANIN BIOSYNTHETIC REGULATOR MDMYB1 POSITIVELY REGULATES ASCORBIC ACID BIOSYNTHESIS IN APPLE

Jianping AN, Xiaofei WANG, Chunxiang YOU, Yujin HAO

Journal Article

Investigation of polyhydroxyalkanoates (PHAs) biosynthesis from mixed culture enriched by valerate-dominant

Jiuxiao Hao, Xiujin Wang, Hui Wang

Journal Article

Assembly of biosynthetic pathways in

Lidan Ye,Xiaomei Lv,Hongwei Yu

Journal Article

PRODUCTION OF NEW WAP-8294A CYCLODEPSIPEPTIDES BY THE BIOLOGICAL CONTROL AGENT LYSOBACTER ENZYMOGENES OH11

Journal Article

Engineering the Biosynthesis of Caffeic Acid in Saccharomyces cerevisiae with Heterologous Enzyme Combinations

Lanqing Liu, Hong Liu, Wei Zhang, Mingdong Yao, Bingzhi Li, Duo Liu, Yingjin Yuan

Journal Article

Pseudomonas Cyclic Lipopeptide Medpeptin: Biosynthesis and Modulation of Plant Immunity

Yi-Lin Gu, Jun-Zhou Li, Yan Li, Shen Cong, Jing Wang, Yi-Nan Ma, Hai-Lei Wei

Journal Article

Biosynthesis and Immunological Evaluation of a Dual-Antigen Nanoconjugate Vaccine against Brucella

Jing Huang, Yufei Wang, Kangfeng Wang, Shulei Li, Peng Sun, Yan Guo, Jiankai Liu, Ruifu Yang, Ming Zeng, Chao Pan, Hengliang Wang, Li Zhu

Journal Article