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Journal Article 5

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2020 2

2019 1

2017 1

2013 1

Keywords

biotransformation 3

Biodegradation 2

β-glucuronidase 1

Aeromonas hydrophila DN322p 1

Arsenic 1

Bioremediation 1

Biosurfactant 1

Biotransformation 1

Glycerol 1

Microbes 1

Microbial metabolism 1

Rhamnolipid 1

Switch 1

Wetland 1

biosorption 1

crystal violet 1

decolorization 1

enzyme-inorganic hybrid nanoflower 1

glycyrrhizin 1

glycyrrtinic acid 3-O-mono-β-D-glucuronide 1

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Biosorption and biotransformation of crystal violet by

Tao PAN, Suizhou REN, Jun GUO, Meiying XU, Guoping SUN

Frontiers of Environmental Science & Engineering 2013, Volume 7, Issue 2,   Pages 185-190 doi: 10.1007/s11783-012-0435-6

Abstract: DN322p, an offspring of DN322, has the capacity to adsorb and decolorize triphenylmethane dyes in wastewater simultaneously. As a common triphenylmethane dye, crystal violet (CV) was chosen to test the decolorization characteristics of DN322p. Within 0.5 h, the strain DN322p adsorbed a large amount of CV, producing a deep-colored cell pellet and colorless supernatant. The colors of the cell pellet and supernatant lightened over time. The supernatant and dichloromethane extract of the cell pellet both showed conspicuous CV and leuco CV (LCV) characteristic absorbance peaks at 590 nm and 260 nm, respectively, in the UV-vis spectral analysis. This finding indicated that the DN322p cells can adsorb the two dyes. A 99% (w/w) decolorization rate was achieved within 2.5 h with shaking at 30°C for 50 mg CV·L . High Performance Liquid Chromatography (HPLC) analysis of the dichloromethane extract of the supernatant and cell pellet confirmed that CV was mainly converted into its leuco form. Dead cells had a similar adsorption capacity with living cells. About 90% of CV in the dye solution (50 mg·L ) was removed by autoclaved cells with an optical delnsity at 600 nm (OD ) above 1.0.

Keywords： crystal violet     decolorization     biosorption     biotransformation     Aeromonas hydrophila DN322p    

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Microbial mediated arsenic biotransformation in wetlands

Si-Yu Zhang, Paul N. Williams, Jinming Luo, Yong-Guan Zhu

Frontiers of Environmental Science & Engineering 2017, Volume 11, Issue 1, doi: 10.1007/s11783-017-0893-y

Abstract: Environmental factors affecting arsenic microbial biotransformation are summarized.

Keywords： Arsenic     Wetland     Microbes     Switch    

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A newly defined dioxygenase system from Mycobacterium vanbaalenii PYR-1 endowed with an enhanced activity of dihydroxylation of high-molecular-weight polyaromatic hydrocarbons

Yiquan Wu, Ying Xu, Ningyi Zhou

Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 1, doi: 10.1007/s11783-019-1193-5

Abstract: equipped with an exogenous electron transport chain components PhdCD from Nocardioides sp. strain KP7 by biotransformation

Keywords： Biodegradation     polyaromatic hydrocarbons     biotransformation     ring-hydroxylating dioxygenase system    

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Rhamnolipids Induced by Glycerol Enhance Dibenzothiophene Biodegradation in Burkholderia sp. C3 Article

Camila A. Ortega Ramirez, Abraham Kwan, Qing X. Li

Engineering 2020, Volume 6, Issue 5,   Pages 533-540 doi: 10.1016/j.eng.2020.01.006

Abstract:

In highly urbanized areas, pollution from anthropogenic activities has compromised the integrity of the land, decreasing soil availability for agricultural practices. Dibenzothiophene (DBT) is a heterocyclic aromatic hydrocarbon frequently found in urbanized areas, and is often used as a model chemical to study the microbial transformation of pollutants. The potential for human exposure and its health risk makes DBT a chemical of concern; thus, it needs to be environmentally managed. We utilized glycerol to stimulate Burkholderia sp. C3 in order to degrade DBT in respect to ① DBT biodegradation kinetics, ② bacterial growth, ③ rhamnolipid (RL) biosynthesis, and ④ RL secretion. Under an optimum glycerol-to-DBT molar ratio, the DBT biodegradation rate constants increased up to 18-fold and enhanced DBT biodegradation by 25%–30% at day 1 relative to cultivation with DBT alone. This enhancement was correlated with an increase in bacterial growth and RL biosynthesis. Proteomics studies revealed the enzymes involved in the upper and main steps of RL biosynthesis. The RL congeners Rha-C10-C10, Rha-Rha-C10-C10, Rha-Rha-C10-C12, and Rha-Rha-C12-C12 were identified in the medium supplemented with glycerol and DBT, whereas only Rha-C12-C12 was identified in cultures without glycerol or with RL inhibitors. The studies indicated that glycerol enhances DBT biodegradation via increased RL synthesis and bacterial growth. The results warrant further studies of environmental biostimulation with glycerol to advance bioremediation technologies and increase soil availability for agricultural purposes.

Keywords： Biodegradation     Bioremediation     Biosurfactant     Biotransformation     Glycerol     Microbial metabolism     Rhamnolipid    

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Construction of a CaHPO4-PGUS1 hybrid nanoflower through protein-inorganic self-assembly, and its application in glycyrrhetinic acid 3-O-mono-β-D-glucuronide preparation

Tian Jiang, Yuhui Hou, Tengjiang Zhang, Xudong Feng, Chun Li

Frontiers of Chemical Science and Engineering 2019, Volume 13, Issue 3,   Pages 554-562 doi: 10.1007/s11705-019-1834-z

Abstract: Glycyrrhetinic acid 3- -mono- -D-glucuronide (GAMG), an important pharmaceutical intermediate and functional sweetener, has broad applications in the food and medical industries. A green and cost-effective method for its preparation is highly desired. Using site-directed mutagenesis, we previously obtained a variant of -glucuronidase from Li-3 (PGUS1), which can specifically transform glycyrrhizin (GL) into GAMG. In this study, a facile method was established to prepare a CaHPO -PGUS1 hybrid nanoflower for enzyme immobilization, based on protein-inorganic hybrid self-assembly. Under optimal conditions, 1.2 mg of a CaHPO -PGUS1 hybrid nanoflower precipitate with 71.2% immobilization efficiency, 35.60 mg∙g loading capacity, and 118% relative activity was obtained. Confocal laser scanning microscope and scanning electron microscope results showed that the enzyme was encapsulated in the CaHPO -PGUS1 hybrid nanoflower. Moreover, the thermostability of the CaHPO -PGUS1 hybrid nanoflower at 55°C was improved, and its half-life increased by 1.3 folds. Additionally, the CaHPO -PGUS1 hybrid nanoflower was used for the preparation of GAMG through GL hydrolysis, with the conversion rate of 92% in 8 h, and after eight consecutive runs, it had 60% of its original activity.

Keywords： β-glucuronidase     enzyme-inorganic hybrid nanoflower     biotransformation     glycyrrhizin     glycyrrtinic    

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Title Author Date Type Operation

Biosorption and biotransformation of crystal violet by

Tao PAN, Suizhou REN, Jun GUO, Meiying XU, Guoping SUN

Journal Article

Microbial mediated arsenic biotransformation in wetlands

Si-Yu Zhang, Paul N. Williams, Jinming Luo, Yong-Guan Zhu

Journal Article

A newly defined dioxygenase system from Mycobacterium vanbaalenii PYR-1 endowed with an enhanced activity of dihydroxylation of high-molecular-weight polyaromatic hydrocarbons

Yiquan Wu, Ying Xu, Ningyi Zhou

Journal Article

Rhamnolipids Induced by Glycerol Enhance Dibenzothiophene Biodegradation in Burkholderia sp. C3

Camila A. Ortega Ramirez, Abraham Kwan, Qing X. Li

Journal Article

Construction of a CaHPO4-PGUS1 hybrid nanoflower through protein-inorganic self-assembly, and its application in glycyrrhetinic acid 3-O-mono-β-D-glucuronide preparation

Tian Jiang, Yuhui Hou, Tengjiang Zhang, Xudong Feng, Chun Li

Journal Article