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Journal Article 3

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2017 2

2000 1

Keywords

heterozygosity 2

Puccinia striiformis f. sp. tritici 1

allele frequency 1

genotype 1

races of Apis mellifera malate dehydrogenase (MDH) 1

selfing 1

virulence inheritance 1

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Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of

Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 1,   Pages 48-58 doi: 10.15302/J-FASE-2016114

Abstract: Therefore, investigating the heterozygosity of the pathogen is important because of rapid virulence changeswas selfed on to determine the heterozygosity for avirulence/virulence loci.

Keywords: tritici     selfing     heterozygosity     virulence inheritance    

Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 1,   Pages 121-122 doi: 10.15302/J-FASE-2017142

Studies on Malate Dehydrogenase Ⅱ (MDH Ⅱ ) Isozymes in Different Races of Apis mellifera ligustica

Chen Shenglu,Bao Xiuliang,Su Songkun,Liu Yanhe

Strategic Study of CAE 2000, Volume 2, Issue 1,   Pages 57-61

Abstract:

The young workers (in three days) of five races of Apis mellifera ligustica- ZAU — 1 Italian honey bee (Ea), Chinese Italian honey bee (Eb), American Italian honey bee (Em), Italian Italian honey bee (Ee) and Australian Italian honey bee (Eo) have been used to study the genotype, genotype frequency, allele frequency, heterozygous and homzygous degrees of the malate dehydrogenase Ⅱ (MDH Ⅱ ) isozymes with polyacrylamide gel isoelectrofocusing (IEF-PAGE). There are six genotypes (a/a, a/b, a/c, b/b, b/c, c/ c) in MDH Ⅱ that is coded by three alleles: a,b and c. All of them are found in Ea, Eb and Ee; four (a/b, a/c, b/c, c/c) are found in Eo; three (a/c, b/c, c/c) in Em.The genotype frequencies are aa (0.022), ab (0.028), ac (0.296), bb (0.007), be (0.140), cc (0.508) in Ea; aa (0.003), ab (0.011), ac(0.117), bb (0.006), be (0.156), cc (0.706) in Eb; aa (0.033), ab (0.203), ac (0.224), bb(0.021), be (0.252), cc (0.267) in Ee; aa (0.000), ab (0.000), ac (0.313), bb (0.000), be(0.253), cc (0.433) in Em; aa (0.000), ab (0.006), ac (0.011), bb (0.000), be (0.172), cc (0.811)in Eo. The allele frequencies are a (0.184), b (0.091), c (0.726) in Ea; a (0.067), b (0.090), c (0.717) in Em; a (0.008), b (0.089), c (0.903) in Eo. The heterozygosities (H) of the MDH Ⅱ locus equal to 0.464 in Ea; 0.284 in Eb; 0.679 in Ee; 0.566 in Em; 0.189 in Eo.

The results of test-independence show that there are significant differences in the distributions of genotype frequency, gene frequency, heterozygous and homozygous degrees among five races of Apis mellifera.

Keywords: races of Apis mellifera malate dehydrogenase (MDH)     genotype     allele frequency     heterozygosity    

Title Author Date Type Operation

Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of

Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG

Journal Article

Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

Journal Article

Studies on Malate Dehydrogenase Ⅱ (MDH Ⅱ ) Isozymes in Different Races of Apis mellifera ligustica

Chen Shenglu,Bao Xiuliang,Su Songkun,Liu Yanhe

Journal Article