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Efficient acetoin production from pyruvate by engineered whole-cell biocatalysis

Frontiers of Chemical Science and Engineering Pages 425-436 doi: 10.1007/s11705-022-2229-0

Abstract: Halomonas bluephagenesisi>, a chassis for next generation of industrial biotechnology, has advantagesHere, αi>-acetolactate synthase and αi>-acetolactate decarboxylase from Bacillus subtilisi> 168 were co-expressed in H. bluephagenesisi> to produce acetoin from pyruvate.After reaction condition optimization and further increase of αi>-acetolactate decarboxylase expressionThis work therefore provided an efficient H. bluephagenesisi> whole-cell catalysis with a broad

Keywords： acetoin pyruvate αi>-acetolactate synthetase αi>-acetolactate decarboxylase Halomonasbluephagenesisi> whole-cell biocatalysis

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The Potential Role of Powdery Mildew-Resistance Gene Pm40i> in Chinese Wheat-Breeding Programs inthe Post-Pm21i> Era Perspective

Shengwen Tang,Yuting Hu,Shengfu Zhong,Peigao Luo

Engineering 2018, Volume 4, Issue 4, Pages 500-506 doi: 10.1016/j.eng.2018.06.004

Abstract:

Powdery mildew, which is caused by Blumeria graminis f. sp. tritici (Bgt), is an important leaf disease that affects wheat yield. Powdery mildew-resistance (Pm) gene Pm21 was first transferred into wheat in the 1980s, by translocating the Heuchera villosa chromosome arm 6VS to the wheat chromosome arm 6AL (6VS·6AL). Recently, new Bgt isolates that are virulent to Pm21 have been identified in some wheat fields, indicating that wheat breeders should be aware of the risk of deploying Pm21, although pathological details regarding these virulent isolates still remain to be discovered. Pm40 was identified and mapped on the wheat chromosome arm 7BS from several wheat lines developed from the progenies of a wild cross between wheat and Thinopyrum intermedium. Pm40 offers a broad spectrum of resistance to Bgt, which suggests that it is likely to provide potentially durable resistance. Cytological methods did not detect any large alien chromosomal segment in the wheat lines carrying Pm40. Lines with Pm40 and promising agronomical traits have been released by several wheat-breeding programs in the past several years. Therefore, we believe that Pm40 will play a role in powdery mildew-resistance wheat breeding after Pm21 resistance is overcome by Bgt isolates. In addition, both Pm21 and Pm40 were derived from alien species, suggesting that the resistance genes derived from alien species are potentially more durable or effective than those identified from wheat.

Keywords： Wheat Powdery mildew Pm21i> Pm40i> Alien species Native resistance

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Development of a new method for RMR and Q classification method to optimize support system in tunneling

Asghar RAHMATI,Lohrasb FARAMARZI,Manouchehr SANEI

Frontiers of Structural and Civil Engineering 2014, Volume 8, Issue 4, Pages 448-455 doi: 10.1007/s11709-014-0262-x

Abstract: Rock mass classification system is very suitable for various engineering design and stability analysis. classification method is confirmed by Japan Highway Public Corporation that this method can figure out either strength or deformability of rock mass, further appropriating the amount of rock bolts, thickness of shotcrete, and size of pitch of steel ribs just after the blasting procedure. Based on these advantages of method, in this study, according to data of five deep and long tunnels in Iran, two equations for estimating the value of method from and classification systems were developed. These equations as a new method were able to optimize the support system for and classification systems. From classification and its application in these case studies, it is pointed out that the method for the design of support systems in underground working is more reliable than the and classification systems.

Keywords： JHi> classification Qi> and RMRi> classification new method

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Presence and Antimicrobial Susceptibility of RE-cmeABCi>-Positive Campylobacteri> Isolated Article

Dejun Liu, Weiwen Liu, Xing Li, Hong Yao, Zhangqi Shen, Yang Wang, Jianzhong Shen

Engineering 2020, Volume 6, Issue 1, Pages 34-39 doi: 10.1016/j.eng.2019.10.013

Abstract:

Campylobacter spp. (C. spp.) are the leading cause of human gastroenteritis worldwide. RE-CmeABC is a newly identified resistance-enhancing multidrug efflux pump of C. spp. that confers high-level resistance to fluoroquinolones, phenicols, macrolides, and tetracyclines, all of which are critical drugs in both human and veterinary medicine. In this study, we analyzed the presence and antimicrobial susceptibility of RE-cmeABC-positive Campylobacter isolates of food-animal origin from three representative regions (Shandong, Shanghai, and Guangdong) in China over three successive years, from 2014 to 2016. A total of 1088 Campylobacter isolates (931 C. coli and 157 C. jejuni) were recovered from the RE-cmeABC screening. We detected 122 (11.2%) RE-cmeABC-positive isolates of chicken origin, including 111 (70.7%) C. jejuni and 11 (1.2%) C. coli. This multidrug efflux pump is more prevalent among C. jejuni than C. coli. The level of resistance was significantly different in 111 RE-cmeABC-positive C. jejuni versus 46 RE-cmeABC-negative C. jejuni for florfenicol, clindamycin, and erythromycin (P < 0.05), but not for ciprofloxacin (CIP), tetracycline (TET), and gentamicin. However, the isolates harboring RE-cmeABC could shift the minimum inhibitory concentration distribution to the higher range for CIP and TET. Pulsed-field gel electrophoresis (PFGE) analysis suggested that horizontal transmission might be involved in the dissemination of RE-cmeABC in Shanghai and Guangdong, while clonal expansion was predominant in Shandong. Three isolates shared the indiscriminate PFGE types of RE-cmeABC-positive C. jejuni isolates in Shanghai and Guangdong, and four isolates in Shanghai and Shandong. Our study suggests the possibility of a wide dissemination of RE-cmeABC in Campylobacter of food-animal origin, which would pose a significant threat to public health.

Keywords： RE-cmeABCi> Campylobacteri> Ciprofloxacin Florfenicol

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Traces and echoes of De Architecturai> by Marcus Vitruvius Pollio in the work of Xu Guangqi in 17th

Michela CIGOLA,Yibing FANG

Frontiers of Mechanical Engineering 2016, Volume 11, Issue 1, Pages 3-11 doi: 10.1007/s11465-015-0359-5

Abstract: the 16th and 17th centuries by analyzing a book of Western technology that he wrote, namely, Taixi Shuifai> (On Western Hydraulicsi>).Taixi Shuifai>.found that Archimedes’ screw and Ctesibius’ machine, which are included in Vitruvius’ De Architecturai> volumes, also appear in the work of Xu Guangqi.

Keywords： Vitruvius’ De Architecturai> Xu Guangqi’s Taixi Shuifai> Jesuit missionaries Italian Renaissance

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Novel variants in and and recurrent variant in underlying epidermolysis bullosa in five Chinese families

Frontiers of Medicine Pages 808-814 doi: 10.1007/s11684-021-0878-x

Abstract: Chinese families with EB, and eight variants including a novel nonsense variant (c.47G>A, p.W16*) in LAMA3i>, a known recurrent variant (c.74C>T, p.P25L) in KRT5i>, 2 novel (c.2531T>A, p.V844E; cCOL7A1 i>were detected.Notably, this study identified a nonsense variant in LAMA3i> that causes EB within the Chinese populationand revealed that this variant resulted in a reduction in LAMA3i> mRNA and protein expression levels

Keywords： epidermolysis bullosa LAMA3i> COL7A1i> KRT5i> Chinese families

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A highly efficient methodology for the preparation of

Yongxin Zhang, Shucheng Wang, Yaodong Huang

Frontiers of Chemical Science and Engineering 2021, Volume 15, Issue 3, Pages 679-686 doi: 10.1007/s11705-020-1979-9

Abstract: A convenient and highly efficient method is described for the synthesis of -methoxycarbazole derivatives, including those with sterically demanding, benzannulated, or strongly electron-donating or -withdrawing substituents. Various -methoxycarbazole derivatives were directly prepared in good-to-moderate yields by the Pd (dba) CHCl /9,9-dimethyl-4,5-bis(diphenylphosphino)xanthene-catalyzed reactions of the corresponding dibromobiphenyl compounds and methoxya-mine. Based on this methodology, the first total synthesis of 3,3′-[oxybis(methylene)]bis(9-methoxy-9 -carbazole), an antimicrobial dimeric carbazole alkaloid previously isolated from the stem bark of , was achieved in 18% yield over seven steps from 1,2-dibromobenzene.

Keywords： Ni>-methoxyl carbazole dimeric Ni>-methoxyl carbazole alkaloid total synthesis double Ni>-arylation of methoxyamine

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RR1i> and RR2i> gene deletion affects the immunogenicity of a live attenuated pseudorabies

Shijun YAN,He YAN,Chaolin ZHANG,Tongyan WANG,Qingyuan YANG,Zhe SUN,Yan XIAO,Feifei TAN,Xiangdong LI,Kegong TIAN

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 1, Pages 81-86 doi: 10.15302/J-FASE-2016088

Abstract: As virulence-determining genes, and encode the small subunit and large subunit of viral ribonucleotide reductase (RR) in pseudorabies virus which have been extensively studied in mice. However, their role in pigs has not been adequately investigated. In this study, we deleted and genes based on a / / triple gene-deleted pseudorabies virus and tested its efficacy in pigs as a vaccine candidate. The rescued virus showed similar growth properties and plaque size as its parent strain. In an animal study, the virus could elicit humoral immune responses shown by generation of gB-specific antibodies and virus neutralizing antibodies. However, vaccination could not provide protection against virulent pseudorabies virus challenge since vaccinated pigs showed clinical pseudorabies-specific syndromes. The deficiency in protection may due to the generation of late and low levels of gB antibodies and virus neutralizing antibodies.

Keywords： pseudorabies virus RR1i> and RR2i> ribonucleotide reductase vaccine candidate

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Accounting for the uncertainties in the estimation of average shear wave velocity using – correlations

Frontiers of Structural and Civil Engineering 2021, Volume 15, Issue 5, Pages 1199-1208 doi: 10.1007/s11709-021-0749-1

Abstract: Shear wave velocity ( Vi> _S) is a key parameter in such analysis, although the economyVarious Vi> _S–SPT– Ni> correlations are routinely incorporated in seismic hazardanalysis to estimate the value of Vi> _S.i> _S calculations.The scattering nature of the Vi> _S values estimated using different Vi> _{S

Keywords：

uncertainties


Vi> _S– Ni> correlations


Vi> _S30


SPT data


statistical


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Thermal and Mechanical Properties Optimization of ABi>O₄ Type EuNbO₄ By theBi>-Site Substitution of Ta Article

Lin Chen, Jing Feng

Engineering 2020, Volume 6, Issue 2, Pages 177-184 doi: 10.1016/j.eng.2019.12.006

Abstract:

Ferroelastic ABO₄ type RETaO₄ and RENbO₄ ceramics (where RE stands for rare earth) are being investigated as promising thermal barrier coatings (TBCs), and the mechanical properties of RETaO₄ have been found to be better than those of RENbO₄. In this work, B-site substitution of tantalum (Ta) is used to optimize the thermal and mechanical properties of EuNbO₄ fabricated through a solid-state reaction (SSR). The crystal structure is clarified by means of X-ray diffraction (XRD) and Raman spectroscopy; and the surface microstructure is surveyed via scanning electronic microscope (SEM). The Young's modulus and the thermal expansion coefficient (TEC) of EuNbO₄ are effectively increased; with respective maximum values of 169 GPa and 11.2×10^–6 K^–1 (at 1200 °C). The thermal conductivity is reduced to 1.52 W·K⁻¹·m⁻¹ (at 700 °C), and the thermal radiation resistance is improved. The relationship between the phonon thermal diffusivity and temperature was established in order to determine the intrinsic phonon thermal conductivity by eliminating the thermal radiation effects. The results indicate that the thermal and mechanical properties of EuNbO₄ can be effectually optimized via the B-site substitution of Ta, and that
this proposed material can be applied as a high-temperature structural ceramic in future.

Keywords： Thermal barrier coatings Rare earth niobates Substitution Thermal conductivity Thermal expansion coefficients Young’s modulus

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NES1i>/KLK10i> and hNISi> gene therapy enhanced iodine-131 internal radiation in PC3 proliferation

Jiajia Hu, Wenbin Shen, Qian Qu, Xiaochun Fei, Ying Miao, Xinyun Huang, Jiajun Liu, Yingli Wu, Biao Li

Frontiers of Medicine 2019, Volume 13, Issue 6, Pages 646-657 doi: 10.1007/s11684-018-0643-y

Abstract: , we supposed to have an “enhanced firepower” effect by combining overexpressed gene therapy and Itomography/computed tomography (micro-PET/CT) imaging showed that the combined gene therapy and Ithat via inhibition of expression, overexpressed might enhance the effect of radiation therapy of I

Keywords： androgen-independent prostate cancer normal epithelial cell-specific 1/kallikrein 10 sodium/iodide symporter radiation therapy proliferation

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Inhibition of NO emission by adding antioxidant mixture in

A. PRABU,R. B. ANAND

Frontiers in Energy 2015, Volume 9, Issue 2, Pages 238-245 doi: 10.1007/s11708-015-0356-8

Abstract: In this paper, the effect of adding an antioxidant mixture in biodiesel as fuel, in a single cylinder, direct injection compression ignition engine was experimentally investigated and the level of pollutants in the exhaust and performance characteristics of the engine were analyzed. Nine test fuels were prepared with three antioxidants, namely, Succinimide (C H NO ), , dimethyl- -phenylenediamine-dihydrochloride (C H Cl N ), and -phenyl- -phenylenediamine (C H NHC H NH ) added to neat biodiesel at 500 parts per million (ppm), 1000 ppm and 2000 ppm and the observed experimental results were compared with those of neat biodiesel and neat diesel as base fuels. The comparison showed that NO emission was reduced drastically for the test fuels with the antioxidant addition of 2000 ppm. The maximum reduction of 10% of NO emission was observed for the antioxidant mixture in neat biodiesel, with a slight increase in unburned HC, CO and smoke opacity. In addition, the obtained experimental results reveal that the addition of two antioxidants as mixture in neat biodiesel caused improved NO emission reduction for all test fuels.

Keywords： NO emission antioxidants Succinimide Ni> Ni>-dimethyl-pi>-phenylenediamine-dihydrochloride Ni>-phenyl-pi>-phenylenediamine

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FESEi>’s Best Papers of 2017

Frontiers of Environmental Science & Engineering 2018, Volume 12, Issue 3, doi: 10.1007/s11783-018-1051-x

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Anti-β glycoprotein I antibodies in complex with β2 glycoprotein I induce platelet activation via tworeceptors: apolipoprotein E receptor 2' and glycoprotein I bα

Wenjing Zhang,Fei Gao,Donghe Lu,Na Sun,Xiaoxue Yin,Meili Jin,Yanhong Liu

Frontiers of Medicine 2016, Volume 10, Issue 1, Pages 76-84 doi: 10.1007/s11684-015-0426-7

Abstract:

Anti-β₂ glycoprotein I (anti-β₂GP I ) antibodies are importantba (GP I bα) in platelet activation induced by the anti-β₂GP I /β₂GP I complex.The interaction between the anti-β₂GP I /β₂GP I complex and plateletsOur results revealed that the anti-β₂GP I /β₂GP I complex was able to

Keywords： anti-β2GP I /β2GP I complex platelet GP I bα apoER2' thrombosis

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A systematic approach in load disaggregation utilizing a multi-stage classification algorithm for consumer electrical appliances classification

Chuan Choong YANG, Chit Siang SOH, Vooi Voon YAP

Frontiers in Energy 2019, Volume 13, Issue 2, Pages 386-398 doi: 10.1007/s11708-017-0497-z

Abstract: The potential to save energy in existing consumer electrical appliances is very high. One of the ways to achieve energy saving and improve energy use awareness is to recognize the energy consumption of individual electrical appliances. To recognize the energy consumption of consumer electrical appliances, the load disaggregation methodology is utilized. Non-intrusive appliance load monitoring (NIALM) is a load disaggregation methodology that disaggregates the sum of power consumption in a single point into the power consumption of individual electrical appliances. In this study, load disaggregation is performed through voltage and current waveform, known as the - trajectory. The classification algorithm performs cropping and image pyramid reduction of the - trajectory plot template images before utilizing the principal component analysis (PCA) and the -nearest neighbor ( -NN) algorithm. The novelty of this paper is to establish a systematic approach of load disaggregation through - trajectory-based load signature images by utilizing a multi-stage classification algorithm methodology. The contribution of this paper is in utilizing the “ -value,” the number of closest data points to the nearest neighbor, in the -NN algorithm to be effective in classification of electrical appliances. The results of the multi-stage classification algorithm implementation have been discussed and the idea on future work has also been proposed.

Keywords： load disaggregation voltage-current (Vi>-Ii>) trajectory multi-stage classification algorithm principal component analysis (PCA) ki>-nearest neighbor (ki>-NN)

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Title Author Date Type Operation

Efficient acetoin production from pyruvate by engineered whole-cell biocatalysis

Journal Article

The Potential Role of Powdery Mildew-Resistance Gene Pm40i> in Chinese Wheat-Breeding Programs inthe Post-Pm21i> Era

Shengwen Tang,Yuting Hu,Shengfu Zhong,Peigao Luo

Journal Article

Development of a new method for RMR and Q classification method to optimize support system in tunneling

Asghar RAHMATI,Lohrasb FARAMARZI,Manouchehr SANEI

Journal Article

Presence and Antimicrobial Susceptibility of RE-cmeABCi>-Positive Campylobacteri> Isolated

Dejun Liu, Weiwen Liu, Xing Li, Hong Yao, Zhangqi Shen, Yang Wang, Jianzhong Shen

Journal Article

Traces and echoes of De Architecturai> by Marcus Vitruvius Pollio in the work of Xu Guangqi in 17th

Michela CIGOLA,Yibing FANG

Journal Article

Novel variants in and and recurrent variant in underlying epidermolysis bullosa in five Chinese families

Journal Article

A highly efficient methodology for the preparation of

Yongxin Zhang, Shucheng Wang, Yaodong Huang

Journal Article

RR1i> and RR2i> gene deletion affects the immunogenicity of a live attenuated pseudorabies

Shijun YAN,He YAN,Chaolin ZHANG,Tongyan WANG,Qingyuan YANG,Zhe SUN,Yan XIAO,Feifei TAN,Xiangdong LI,Kegong TIAN

Journal Article

Accounting for the uncertainties in the estimation of average shear wave velocity using – correlations

Journal Article

Thermal and Mechanical Properties Optimization of ABi>O₄ Type EuNbO₄ By theBi>-Site Substitution of Ta

Lin Chen, Jing Feng

Journal Article

NES1i>/KLK10i> and hNISi> gene therapy enhanced iodine-131 internal radiation in PC3 proliferation

Jiajia Hu, Wenbin Shen, Qian Qu, Xiaochun Fei, Ying Miao, Xinyun Huang, Jiajun Liu, Yingli Wu, Biao Li

Journal Article

Inhibition of NO emission by adding antioxidant mixture in

A. PRABU,R. B. ANAND

Journal Article

FESEi>’s Best Papers of 2017

Journal Article

Anti-β glycoprotein I antibodies in complex with β2 glycoprotein I induce platelet activation via tworeceptors: apolipoprotein E receptor 2' and glycoprotein I bα

Wenjing Zhang,Fei Gao,Donghe Lu,Na Sun,Xiaoxue Yin,Meili Jin,Yanhong Liu

Journal Article

A systematic approach in load disaggregation utilizing a multi-stage classification algorithm for consumer electrical appliances classification

Chuan Choong YANG, Chit Siang SOH, Vooi Voon YAP

Journal Article

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