Production of rhamnolipids-producing enzymes of

2017年第11卷第1期

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《化学科学与工程前沿（英文）》 >> 2017年第11卷第1期 doi: 10.1007/s11705-017-1637-z

Production of rhamnolipids-producing enzymes of

Institute of Chemical & Engineering Sciences, Agency for Science, Technology and Research, Jurong Island 627833, Singapore

录用日期： 2017-02-28 发布日期： 2017-03-17

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摘要

Rhamnolipids are a class of biosurfactants that have a great potential to be used in industries. Five proteins/enzymes, namely RhlA, RhlB, RhlC, RhlG and RhlI, are critical for the production of rhamnolipids in . Four of the 5 proteins except RhlC were successfully over-expressed in and three of them (RhlA, RhlB and RhlI) were purified and obtained in milligram quantities. The purified proteins were shown to be folded in solution. Homology models were built for RhlA, RhlB and RhlI. These results lay a basis for further structural and functional characterization of these proteins to favor the construction of super strains for rhamnolipids production.

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rhamnolipids ; Pseudomonas ; RhlA ; RhlB ; RhlI ; protein folding