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《化学科学与工程前沿(英文)》 >> 2023年 第17卷 第4期 doi: 10.1007/s11705-022-2229-0

Efficient acetoin production from pyruvate by engineered whole-cell biocatalysis

收稿日期: 2022-04-23 录用日期: 2023-01-17 发布日期: 2023-01-17

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摘要

Acetoin is an important platform chemical, which has a wide range of applications in many industries. Halomonas bluephagenesis, a chassis for next generation of industrial biotechnology, has advantages of fast growth and high tolerance to organic acid salts and alkaline environment. Here, α-acetolactate synthase and α-acetolactate decarboxylase from Bacillus subtilis 168 were co-expressed in H. bluephagenesis to produce acetoin from pyruvate. After reaction condition optimization and further increase of α-acetolactate decarboxylase expression, acetoin production and yield were significantly enhanced to 223.4 mmol·L–1 and 0.491 mol·mol–1 from 125.4 mmol·L–1 and 0.333 mol·mol–1, respectively. Finally, the highest titer of 974.3 mmol·L–1 (85.84 g·L–1) of acetoin was accumulated from 2143.4 mmol·L–1 (188.6 g·L–1) of pyruvic acid within 8 h in fed-batch bioconversion under optimal reaction conditions. Moreover, the reusability of the cell catalysis was also tested, and the result illustrated that the whole-cell catalysis obtained 433.3, 440.2, 379.0, 442.8 and 339.4 mmol·L–1 (38.2, 38.8, 33.4, 39.0 and 29.9 g·L–1) acetoin in five repeated cycles under the same conditions. This work therefore provided an efficient H. bluephagenesis whole-cell catalysis with a broad development prospect in biosynthesis of acetoin.

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