摘要
A promising microalgal strain isolated from fresh water, which can grow both autotrophically on inorganic carbon under lighting and heterotrophically on organic carbon without lighting, was identified as sp. USTB-01 with the phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences. In the heterotrophic batch culture, more than 20.0 g·L of cell dry weight concentration (DWC) of sp. USTB-01 was obtained at day 5, and which was used directly to seed the autotrophic culture. A novel fermentor-helical combined photobioreactor was established and used to cultivate sp. USTB-01 for the fixation of carbon dioxide (CO ). It showed that the autotrophic growth of sp. USTB-01 in the combined photobioreactor was more effective than that in the fermentor alone and the maximum DWC of 2.5 g·L was obtained at day 6. The highest CO fixation of 95% appeared on day 1 in the exponential growth phases of sp. USTB-01 and 49.8% protein was found in the harvested microalgal cells.
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