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《工程(英文)》 >> 2017年 第3卷 第1期 doi: 10.1016/J.ENG.2017.01.011

人体胃肠道–菌群相互作用的工程学研究模型

a Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Esch-sur-Alzette, L 4362, Luxembourg
b Center for Applied Nanobioscience and Medicine, University of Arizona, Tucson, AZ 85721, USA

录用日期: 2017-02-04 发布日期: 2017-02-28

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摘要

研究宿主–胃肠道微生物的相互作用已经成为管理人类健康和疾病的关键组成部分。微生理系统的发展正在为研究人员提供前所未有的对于这种复杂关系的获取和理解。这些系统结合了微型工程、微流体和细胞培养的优点,来创建人类肠道中普遍存在的环境条件。在这里我们提出的HuMiX(人类微生物交联对话) 平台,是一个利用这种多学科方法提供具有代表性的人体胃肠道的体外模型系统,用于研究宿主–微生物分子的相互作用。我们总结了使用该平台获得的概念验证结果,强调其对于大大增强我们对宿主–微生物相互作用了解的潜力,且其可能对药物、食品和营养以及医疗保健行业产生的巨大影响。同时讨论了这些技术面临的一些关键问题和挑战。

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参考文献

[ 1 ] Sender R, Fuchs S, Milo R. Revised estimates for the number of human and bacteria cells in the body. PLoS Biol 2016;14(8):e1002533 链接1

[ 2 ] Shreiner AB, Kao JY, Young VB. The gut microbiome in health and in disease. Curr Opin Gastroenterol 2015;31(1):69–75 链接1

[ 3 ] Pflughoeft KJ, Versalovic J. Human microbiome in health and disease. Annu Rev Pathol 2012;7(1):99–122 链接1

[ 4 ] Frank DN, St Amand AL, Feldman RA, Boedeker EC, Harpaz N, Pace NR. Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases. Proc Natl Acad Sci USA 2007;104(34):13780–5 链接1

[ 5 ] Cryan JF, Dinan TG. Mind-altering microorganisms: the impact of the gut microbiota on brain and behaviour. Nat Rev Neurosci 2012;13(10):701–12 链接1

[ 6 ] Sobhani I, Tap J, Roudot-Thoraval F, Roperch JP, Letulle S, Langella P, et alMicrobial dysbiosis in colorectal cancer (CRC) patients. PLoS One 2011;6(1):e16393 链接1

[ 7 ] Carding S, Verbeke K, Vipond DT, Corfe BM, Owen LJ. Dysbiosis of the gut microbiota in disease. Microb Ecol Health Dis 2015;26:26191.25651997 链接1

[ 8 ] Shen J, Obin MS, Zhao L. The gut microbiota, obesity and insulin resistance. Mol Aspects Med 2013;34(1):39–58 链接1

[ 9 ] Naseer MI, Bibi F, Alqahtani MH, Chaudhary AG, Azhar EI, Kamal MA, et alRole of gut microbiota in obesity, type 2 diabetes and Alzheimer’s disease. CNS Neurol Disord Drug Targets 2014;13(2):305–11 链接1

[10] Azcárate-Peril MA, Sikes M, Bruno-Bárcena JM. The intestinal microbiota, gastrointestinal environment and colorectal cancer: a putative role for probiotics in prevention of colorectal cancer? Am J Physiol Gastrointest Liver Physiol 2011;301(3):G401–24 链接1

[11] Scheperjans F, Aho V, Pereira PA, Koskinen K, Paulin L, Pekkonen E, et alGut microbiota are related to Parkinson’s disease and clinical phenotype. Mov Disord 2015;30(3):350–8 链接1

[12] Panzer AR, Lynch SV. Influence and effect of the human microbiome in allergy and asthma. Curr Opin Rheumatol 2015;27(4):373–80 链接1

[13] Roume H, Muller EE, Cordes T, Renaut J, Hiller K, Wilmes P. A biomolecular isolation framework for eco-systems biology. ISME J 2013;7(1):110–21 链接1

[14] Wikoff WR, Anfora AT, Liu J, Schultz PG, Lesley SA, Peters EC, et alMetabolomics analysis reveals large effects of gut microflora on mammalian blood metabolites. Proc Natl Acad Sci USA 2009;106(10):3698–703 链接1

[15] Heintz-Buschart A, May P, Laczny CC, Lebrun LA, Bellora C, Krishna A, et alIntegrated multi-omics of the human gut microbiome in a case study of familial type 1 diabetes. Nat Microbiol 2016;2:16180 链接1

[16] Fritz JV, Desai MS, Shah P, Schneider JG, Wilmes P. From meta-omics to causality: experimental models for human microbiome research. Microbiome 2013;1(1):14 链接1

[17] Hapfelmeier S, Lawson MA, Slack E, Kirundi JK, Stoel M, Heikenwalder M, et alReversible microbial colonization of germ-free mice reveals the dynamics of IgA immune responses. Science 2010;328(5986):1705–9 链接1

[18] Parlesak A, Haller D, Brinz S, Baeuerlein A, Bode C. Modulation of cytokine release by differentiated CACO-2 cells in a compartmentalized coculture model with mononuclear leucocytes and nonpathogenic bacteria. Scand J Immunol 2004;60(5):477–85 链接1

[19] Nguyen TLA, Vieira-Silva S, Liston A, Raes J. How informative is the mouse for human gut microbiota research? Dis Model Mech 2015;8(1):1–16 链接1

[20] Arnold JW, Roach J, Azcarate-Peril MA. Emerging technologies for gut microbiome research. Trends Microbiol 2016;24(11):887–901 链接1

[21] Sung JH, Yu J, Luo D, Shuler ML, March JC. Microscale 3-D hydrogel scaffold for biomimetic gastrointestinal (GI) tract model. Lab Chip 2011;11(3):389–92 链接1

[22] Kim HJ, Huh D, Hamilton G, Ingber DE. Human gut-on-a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow. Lab Chip 2012;12(12):2165–74 链接1

[23] Kim HJ, Li H, Collins JJ, Ingber DE. Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip. Proc Natl Acad Sci USA 2016;113(1):E7–15 链接1

[24] Marzorati M, Vanhoecke B, De Ryck T, Sadaghian Sadabad M, Pinheiro I, Possemiers S, et alThe HMI? module: a new tool to study the host-microbiota interaction in the human gastrointestinal tract in vitro. BMC Microbiol 2014;14:133 链接1

[25] Shah P, Fritz JV, Glaab E, Desai MS, Greenhalgh K, Frachet A, et alA microfluidics-based in vitro model of the gastrointestinal human-microbe interface. Nat Commun 2016;7:11535 链接1

[26] Roume H, Muller EE, Cordes T, Renaut J, Hiller K, Wilmes P. A biomolecular isolation framework for eco-systems biology. ISEM J 2013;7(1):110–21 链接1

[27] Sheridan WG, Lowndes RH, Young HL. Intraoperative tissue oximetry in the human gastrointestinal tract. Am J Surg 1990;159(3):314–9 链接1

[28] Schmidt TM, Kao JY. A little O2 may go a long way in structuring the GI microbiome. Gastroenterology 2014;147(5):956–9 链接1

[29] Round JL, Mazmanian SK. The gut microbiota shapes intestinal immune responses during health and disease. Nat Rev Immunol 2009;9(5):313–23 链接1

[30] Lee PJ, Hung PJ, Lee LP. An artificial liver sinusoid with a microfluidic endothelial-like barrier for primary hepatocyte culture. Biotechnol Bioeng 2007;97(5):1340–6 链接1

[31] Jang KJ, Suh KY. A multi-layer microfluidic device for efficient culture and analysis of renal tubular cells. Lab Chip 2010;10:36–42 链接1

[32] Huh D, Matthews BD, Mammoto A, Montoya-Zavala M, Hsin HY, Ingber DE. Reconstituting organ-level lung functions on a chip. Science 2010;328(5986):1662–8 链接1

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