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《工程(英文)》 >> 2020年 第6卷 第1期 doi: 10.1016/j.eng.2019.10.013

2014—2016年食品动物源RE-cmeABC阳性弯曲菌流行与耐药性现状

Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural
University, Beijing 100193, China

收稿日期: 2019-03-08 修回日期: 2019-08-30 录用日期: 2019-10-10 发布日期: 2019-11-01

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摘要

弯曲菌是引起人类胃肠炎的一类重要食源性致病菌。RE-CmeABC是近年来弯曲菌中新发现的一种增强型耐药外排泵,可介导对兽医和人医临床治疗弯曲菌感染的重要药物,如氟喹诺酮类、酰胺醇类、大环内酯类及四环素类等药物的高水平耐药,而目前尚未有对该外排泵的大范围流行病学调查。因此,本研究调查了2014—2016年连续三年间我国山东、上海和广东等三个重点养殖区食品动物源RE-cmeABC阳性弯曲菌的流行与耐药性现状。结果显示,在1088株弯曲菌(931株结肠弯曲菌与157株空肠弯曲菌)中共检测出122株(11.2%)RE-cmeABC阳性菌株,包括111株(70.7%)空肠弯曲菌与11株(1.2%)结肠弯曲菌,空肠弯曲菌中RE-cmeABC的阳性率显著高于结肠弯曲菌。111株RE-cmeABC阳性空肠弯曲菌与46株RE-cmeABC阴性空肠弯曲菌相比在氟苯尼考、克林霉素和红霉素的耐药率上具有显著差异(P < 0.05),而在环丙沙星、四环素和庆大霉素的耐药率上无显著差异。然而,携带RE-cmeABC的菌株可以使环丙沙星与四环素对弯曲菌的MIC值分布趋于更高水平范围。脉冲场凝胶电泳分型(PFGE)结果显示RE-cmeABC阳性菌株在上海和广东地区以水平传播为主,在山东地区以克隆传播为主;三株分离于上海和广东的阳性菌为同一克隆型,4株分离于上海和山东的阳性菌为同一克隆型。本研究表明RE-cmeABC已在我国食品动物源弯曲菌中广泛流行,对公共卫生安全具有潜在威胁。

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