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《工程(英文)》 >> 2020年 第6卷 第5期 doi: 10.1016/j.eng.2019.10.017

注射大鼠脑神经膜的非洲爪蟾卵母细胞——一种创新的体外方法研究拟除虫菊酯对天然态离子通道的影响

a University of Massachusetts, Amherst, MA 01003, USA
b Salve Regina University, Newport, RI 02840, USA

收稿日期: 2018-12-18 修回日期: 2019-08-07 录用日期: 2019-10-08 发布日期: 2020-04-15

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摘要

将大鼠脑神经膜微移植到非洲爪蟾卵母细胞的质膜中,是一种利用标准电生理学方式研究其天然态通道和受体的体外方法。在本文中,我们发现注射了成年大鼠脑神经膜的卵母细胞会引发对河豚毒素敏感的膜去极化内向离子流,经除虫菊酯类杀虫剂氯菊酯和溴氰菊酯处理后,该电流呈浓度依赖性增加。根据我们最初的方案,随着时间的推移,卵母细胞的健康水平会降低,不同批次的不同蛙类卵母细胞间与神经膜的结合程度也不同,这限制了该试验在调控问题上的实用性。对分析程序、数据接受标准和分析方法的一系列更改可显著提高试验的精度,从而提高性能。这些变化促使这种体外方法成为一种毒理学相关分析方法,以研究拟除虫菊酯对取自幼年和成年大鼠脑中的神经膜片段的离子通道的毒性作用。

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