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《工程(英文)》 >> 2022年 第12卷 第5期 doi: 10.1016/j.eng.2020.06.032

电针刺激促进 Mecp2基因敲除的Rett综合征模型大鼠功能恢复

a Bioimaging Center, Shanghai Synchrotron Radiation Facility, Zhang Jiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China
b Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
c Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
d School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035, China
e School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
f College of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
g Cardiology Department, Beijing Hospital, Beijing 100730, China

# These authors contributed equally to this work.

收稿日期: 2020-01-17 修回日期: 2020-04-29 录用日期: 2020-06-08 发布日期: 2022-03-22

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摘要

Rett 综合征(又称雷特综合征)是一种进行性神经发育障碍性疾病,目前尚缺乏有效的治疗手段。已有研究表明,深部脑刺激可以缓解Rett 综合征模型小鼠的一些症状,但这种介入操作需要精细的手术。本研究采用电针刺激(EAS)百会(GV 20)、涌泉(KI 1)和神门(HT 7)穴位,可以减轻Mecp2 基因敲除的Rett 综合征模型大鼠的症状。结果表明,电针不仅延长了Rett 综合征大鼠的生存期,而且提高了其行为能力,包括运动能力、运动协调能力和社会交往能力。进一步研究发现,野生型和Rett 模型大鼠经电针刺激后,中脑黑质、纹状体和大脑皮层中c-Fos 蛋白表达的增加,表明电针刺激能激活这些大鼠脑区神经元的活动,从而促进运动及社交能力的恢复。因此,电针为神经发育性疾病治疗提供了一种安全有效的潜在治疗手段。

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