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《工程(英文)》 >> 2023年 第24卷 第5期 doi: 10.1016/j.eng.2022.08.020

基于集成液体交换的微流控芯片的完整单细胞动态变形测量

a Department of Micro-Nano Mechanical Science and Engineering, Nagoya University, Nagoya 464-8603, Japan
b Department of Mechanical Engineering, The University of Tokyo, Tokyo 113-8656, Japan
c Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai 980-8579, Japan

收稿日期: 2021-07-28 修回日期: 2022-06-09 录用日期: 2022-08-30 发布日期: 2023-02-15

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摘要

本文报道了采用一种集成了力感应和液体交换功能的微流控芯片来测量单细胞力学性能的方法。使用光学镊子操纵和定位在推力探针和力传感器探针之间的单个细胞。这两个芯片上的探针被设计用来捕获和使细胞变形。通过移动由外力驱动的推力探针,而使单个细胞变形。层流在探针之间形成液-液界面以改变细胞外环境。通过控制注入压力来改变界面的位置。通过调整两个正压力和一个负压力来平衡流动的扩散和扰动。在微流控芯片中测定了不同渗透浓度环境下的单个集胞藻(Synechocystis)菌株PCC 6803 的力学性能。在0.3~0.7 s 内实现液体交换过程,同时也显示了单个细胞的动态变形。可以在30 s 内收集不同渗透浓度下两个杨氏模量值的测量结果以及单个细胞在渗透压冲击下的动态响应。研究了野生型(WT)和突变型集胞藻细胞的动态变形,揭示了机械敏感(MS)通道的功能机制。该系统提供了一种监测单个完整细胞响应快速外部渗透变化的实时力学动力学的新方法;因此,该系统为准确描述细胞中MS通道的生理功能提供了新的机会。

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