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《工程(英文)》 >> 2024年 第33卷 第2期 doi: 10.1016/j.eng.2023.09.001

基于离子印迹的二价铜离子非标记检测平台预防听力损失

a The Eighth Affiliated Hospital, Sun Yat-Sen University, Shenzhen 518033, China
b State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital & Advanced Institute for Life and Health, Southeast University, Nanjing 210096, China
c Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Shenzhen University, Shenzhen 518060, China
d Department of Otolaryngology Head and Neck Surgery, Jiangsu Provincial Key Medical Discipline, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China
e Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China
f Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610072, China
g Institute for Stem Cell and Regeneration, Chinese Academy of Science, Beijing 100101, China
h Beijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing 100069, China

# These authors contributed equally to this work.

收稿日期: 2023-07-15 修回日期: 2023-08-21 录用日期: 2023-09-03 发布日期: 2023-09-15

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摘要

铜是一种微量元素,在体内具有重要的生理功能。然而,过量的铜离子(Cu2+)可能会导致严重的健康问题,例如毛细胞凋亡,进而造成听力损失。因此,对Cu2+的测定具有重要意义。在本文中,我们将离子印迹技术与结构色水凝胶微球相结合,制备了基于壳聚糖的离子印迹水凝胶微球(IIHBs),并将其作为低成本、高特异性的Cu2+检测平台。这些IIHBs具有内部贯穿的大孔微纳结构,宏观尺寸均匀、颜色明亮,同时还具备磁响应性。将其孵育于Cu2+溶液中时,IIHB可以识别Cu2+并表现出反射峰变化,从而实现无标记检测。此外,受益于离子印迹技术,IIHBs 显示出良好的特异性和选择性,在Cu2+浓度为100 μmol∙L–1时印迹因子为19.14 。这些特征表明,所开发的 IIHBs有望用于Cu2+检测,进而预防听力损失。

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