RESEARCH ARTICLE
1. National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.2. College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071000, China.3. Animal Husbandry and Veterinary Research Institute of Tianjin, Tianjin 300412, China
录用日期：
2015-01-28
发布日期：
2015-03-10
摘要
This study was conducted to systematically assess the reproductive performance of transgenic ewes. In the transgenic founders (F ) and their positive offspring (F ), hematological and reproductive parameters and the global DNA methylation level in oocytes at various stages were analyzed. The values of the physiological and biochemical parameters determined from the blood samples did not differ significantly between the transgenic and wild-type ewes. Moreover, the transgenic ewes showed reproductive traits similar to the wild-type ewes. These traits included characteristics of puberty, the estrus cycle, estrus duration, gestation, the pregnancy rate and the superovulation response. Additionally, no significant differences were found between transgenic and wild-type ewes in the DNA methylation level of the oocytes at various stages. In summary, the preliminary evidence presented in this paper demonstrates that the presence of the transgene did not affect the reproductive performance in sheep.
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