期刊首页 优先出版 当期阅读 过刊浏览 作者中心 关于期刊 English

2016年 第3卷 第1期

摘要

关键词

相关研究

回顶部

《农业科学与工程前沿（英文）》 >> 2016年 第3卷 第1期 doi: 10.15302/J-FASE-2016088

RR1 and RR2 gene deletion affects the immunogenicity of a live attenuated pseudorabies virus vaccine candidate in natural pig host

1. National Research Center for Veterinary Medicine, Luoyang 471003, China.2. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China

发布日期： 2016-04-07
HTML33 PDF 4 收藏 0

下一篇 上一篇

摘要

As virulence-determining genes, and encode the small subunit and large subunit of viral ribonucleotide reductase (RR) in pseudorabies virus which have been extensively studied in mice. However, their role in pigs has not been adequately investigated. In this study, we deleted and genes based on a / / triple gene-deleted pseudorabies virus and tested its efficacy in pigs as a vaccine candidate. The rescued virus showed similar growth properties and plaque size as its parent strain. In an animal study, the virus could elicit humoral immune responses shown by generation of gB-specific antibodies and virus neutralizing antibodies. However, vaccination could not provide protection against virulent pseudorabies virus challenge since vaccinated pigs showed clinical pseudorabies-specific syndromes. The deficiency in protection may due to the generation of late and low levels of gB antibodies and virus neutralizing antibodies.

相关研究