Changes in bulk soil affect the disease-suppressive rhizosphere microbiome against Fusarium wilt disease
. Jiangsu Provincial Key Laboratory of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Education Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing 210095, China.. School of Life Sciences, Liaoning University, Shenyang 110036, China.. Ecology and Biodiversity Group, Department of Biology, Institute of Environmental Biology, Utrecht University, Utrecht, 3584 CH, The Netherlands.. Department of Plant Science, The Pennsylvania State University, University Park, PA 16802, USA.. Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.. Hainan Key Laboratory for Sustainable Utilization of Tropical Bio-Resources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China录用日期 ： 2020-04-27 发布日期 ：2020-04-27
Harnessing disease-suppressive microbiomes constitutes a promising strategy for optimizing plant growth. However, relatively little information is available about the relationship between bulk and rhizosphere soil microbiomes. Here, the assembly of banana bulk soil and rhizosphere microbiomes was investigated in a monoculture system consisting of bio-organic (BIO) and organic management practices. Applying BIO practice in newly reclaimed fields resulted in a high-efficiency biocontrol rate, thus providing a promising strategy for pre-control of Fusarium wilt disease. The soil microbiota was further characterized by MiSeq sequencing and quantitative PCR. The results indicate that disease suppression was mediated by the structure of a suppressive rhizosphere microbiome with respect to distinct community composition, diversity and abundance. Overall microbiome suppressiveness was primarily related to a particular set of enriched bacterial taxa affiliated with s, , , , and . Finally, structural equation modeling was used to show that the changes in bulk soil bacterial community determined its induced rhizosphere bacterial community, which serves as an important and direct factor in restraining the pathogen. Collectively, this study provides an integrative approach to disentangle the biological basis of disease-suppressive microbiomes in the context of agricultural practice and soil management.