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《中国工程科学》 >> 2014年 第16卷 第5期

hsa-miR-197在子宫肌瘤中的表达及生物信息学特征分析

南京医科大学附属南京市妇幼保健院妇科,南京 210004

资助项目 :国家自然科学基金青年科学基金项目(81202042);南京市卫生局科技发展项目(QRX11212) 收稿日期: 2014-04-03 发布日期: 2014-05-26 15:13:53.000

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摘要

应用实时定量聚合酶链式反应(PCR)技术检测子宫肌瘤组织中的hsa-miR-197 表达水平,并与配对正常子宫肌细胞对比。通过miRbase、UCSC、NCBI等在线数据库获取并分析hsa-miR-197 序列保守性及其基因组特征。选择miRanda、MirTarget2 及TargetScan 三个在线数据库预测hsa-miR-197 的靶基因并取交集以便后续分析。通过基因本体(GO)功能注释、GO富集分析和信号转导通路富集分析初步阐明,hsamiR-197 靶基因参与调控的细胞功能与信号通路。hsa-miR-197 的功能较广泛,参与肿瘤发生的多种生物学过程,提示hsa-miR-197 可能与子宫肌瘤的发病机制密切相关。

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