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《中国工程科学》 >> 2014年 第16卷 第7期

固定化溶菌酶的氧化石墨烯/聚醚砜杂化超滤膜制备及抗菌性能研究

郑州大学化工与能源学院,郑州 450001

资助项目 :国家自然科学基金项目(21106137,21376225);中国博士后基金项目(2013M531684);大学生创新实验训练计划项目(2013xjxm075,2013xjxm072) 收稿日期: 2014-05-08 发布日期: 2014-08-12 14:32:53.000

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摘要

以氧化石墨烯(GO)为固定酶载体,在水溶液中通过静电吸附及氢键作用实现溶菌酶(Ly)的固定化得到GO-Ly。并将已固定化酶的GO为添加剂,以聚醚砜为膜材料,采用相转化法制备杂化超滤膜。考察了添加剂含量对膜形态、亲水性、分离性能、力学性能及抗菌性能的影响。结果表明,GO-Ly 的加入使杂化膜的亲水性及纯水通量得到明显提高,同时拉伸强度也得到一定改善;尤其当GO-Ly 添加量为1.5 %(质量分数)时,膜的纯水通量达到318 L/(m2 · h),并且对聚乙烯醇(PVA 30 000~70 000)的截留率维持在99 %以上,对大肠杆菌的抑菌率可达68 %。

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