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Isolation, characterization and identification of plant growth-promoting rhizobacteria from the rhizosphere RESEARCH ARTICLE
《农业科学与工程前沿（英文）》 2024年 第11卷 第3期 页码 499-513 doi: 10.15302/J-FASE-2024559
This study has isolated, characterized, and identified potential plant growth-promoting rhizobacteria (PGPR) with multiple PGP characteristics (N2-fixation, P- and K-solubilization, IAA, and siderophores production) from the rhizosphere BRIS soil of Acacia mangium. A total of 24 pure colonies were isolated and only 8 colonies were selected for further evaluation of the growth rate in 5% organic molasses medium supplemented with 2% KNO3. Based on the biochemical, potential PGP characteristics and growth performance, 3 superior PGPR strains were selected and identified as Paraburkholderia unamae (UA1), Bacillus amyloliquefaciens (UA6), and Enterobacter asburiae (UAA2) by partial sequencing of the 16S rRNA gene. The selected bacterial strains either in single or mixed (UA1 + UA6 + UAA2) cultures have shown a significant biochemical estimation of the PGP characteristics. Each strain has its own PGPR traits superiority with UA1 showing the best PGP characteristic followed by UA6 and UAA2. The use of mixed bacterial strains was beneficial as it showed the best performance in N2-fixation, siderophores production, and significant effect on corn phenology, growth and yield compared to using a single strain. These types of microbes showed potential to be used as biofertilizer and should be exploited more.
关键词： Biofertilizer corn organic molasses me- dium PGP characteristic phytohormone potassium nitrate siderophores
Meng DUAN, Jin XIE, Xiaomin MAO
《农业科学与工程前沿（英文）》 2019年 第6卷 第2期 页码 144-161 doi: 10.15302/J-FASE-2019258
Based on our previous work modeling crop growth (CropSPAC) and water and heat transfer in the soil-plant-atmosphere continuum (SPAC), the model was improved by considering the effect of plastic film mulching applied to field-grown maize in North-west China. In CropSPAC, a single layer canopy model and a multi-layer soil model were adopted to simulate the energy partition between the canopy and water and heat transfer in the soil, respectively. The maize growth module included photosynthesis, growth stage calculation, biomass accumulation, and participation. The CropSPAC model coupled the maize growth module and SPAC water and heat transfer module through leaf area index (LAI), plant height and soil moisture condition in the root zone. The LAI and plant height were calculated from the maize growth module and used as input for the SPAC water and heat transfer module, and the SPAC module output for soil water stress conditions used as an input for maize growth module. We used , the representation of evaporation resistance, instead of the commonly used evaporation resistance to reflect the change of latent heat flux of soil evaporation under film mulching as well as the induced change in energy partition. The model was tested in a maize field at Yingke irrigation area in North-west China. Results showed reasonable agreement between the simulations and measurements of LAI, above-ground biomass and soil water content. Compared with the original model, the modified model was more reliable for maize growth simulation under film mulching and showed better accuracy for the LAI (with the coefficient of determination = 0.92, the root mean square of error RMSE= 1.23, and the Nush-Suttclife efficiency E = 0.87), the above-ground biomass (with = 0.96, RMSE= 7.17 t·ha and E = 0.95) and the soil water content in 0–1 m soil layer (with = 0.78, RMSE= 49.44 mm and E = 0.26). Scenarios were considered to simulate the influence of future climate change and film mulching on crop growth, soil water and heat conditions, and crop yield. The simulations indicated that the change of LAI, leaf biomass and yield are negatively correlated with temperature change, but the growing degree-days, evaporation, soil water content and soil temperature are positively correlated with temperature change. With an increase in the ratio of film mulching area, the evaporation will decrease, while the impact of film mulching on crop transpiration is not significant. In general, film mulching is effective in saving water, preserving soil moisture, increasing soil surface temperature, shortening the potential growth period, and increasing the potential yield of maize.
关键词： film mulching growth stage leaf area index maize growth water and heat transfer
《农业科学与工程前沿（英文）》 doi: 10.15302/J-FASE-2024556
● Two bean cultivars responded strongly to Rhizobium inoculation in both 2012 and 2013, relative to uninoculated control.
关键词： Bean cultivars grain yield Hawassa Dume Ibbado nodule dry matter nodule number pod number per plant seed number per pod shoot biomass
《环境科学与工程前沿（英文）》 2023年 第17卷 第1期 doi: 10.1007/s11783-023-1609-0
● Improved Cr phytoextration efficiency was achieved by B. cereus inoculation.
关键词： Hexavalent chromium Hyperaccumulator Rhizobacteria Leersia hexandra Swartz Bacillus cereus Consortia
刘德盛,张群
《中国工程科学》 2000年 第2卷 第2期 页码 68-73
目前我国海带养殖面积约1.5万hm2，年产干品27t，占世界海藻总产量的50%。三十烷醇（TA）乳粉可显著提高海带的产量和品质，海带苗经2mg/L TA浸泡2h后放养，能促进假根生长，提高保苗率，增加叶绿素、岩藻黄素含量，促进碳、氮代谢，增加干物质积累。处理比对照平均增收干品6.57t/hm2,增产率为35%。同时，海带可比对照增收碘24.24kg/hm2、褐藻胶1.665t/hm2、甘露醇1.278t/hm2。TA乳粉属国内外首创，具有无毒、高效、成本低、不变质及贮运方便等优点，已在全国海带上推广1.6万hm2,创经济效益3亿多元。
刘德盛,张群,陆东和
《中国工程科学》 2001年 第3卷 第2期 页码 91-94
三十烷醇（TA）是由30个碳原子组成的长链脂肪醇，普遍存在于动植物的蜡质中，常用蜂蜡为原料制取。我国首先在TA剂型研究上取得重大进展，采用CD分子包衣技术研制的TA乳粉属国内外首创，它具有TA乳剂所不具备的增产效果稳定、长期贮存不变质和贮运方便等优点，并开拓TA乳粉在海藻上应用研究的新领域。TA乳粉是一种无毒害、无污染、成本低、效益高的广谱性植物生长调节剂，应用前景广阔。
Toxicity mechanisms of photodegraded polyvinyl chloride nanoplastics on pea seedlings
《环境科学与工程前沿（英文）》 2024年 第18卷 第4期 doi: 10.1007/s11783-024-1809-2
● Photodegraded polyvinyl chloride nanoplastics (PVC-NPs) inhibited pea seedlings’ growth.
关键词： Nanoplasctics Polyvinyl chloride Toxicity mechanisms Plant growth
刘德盛,陆修闽,何明忠,肖华山,范秀珍
《中国工程科学》 2002年 第4卷 第11期 页码 82-88
三十烷醇（TA）乳粉是一种广谱性的植物生长调节剂，1997年被农业部列入全国农牧渔业丰收计划项目后，五六年来在福建省水稻上试验、示范、推广工作取得显著进展。在水稻幼穗分化2—3期和孕穗期分别叶面喷施1～2 mg/L TA一次，能促进稻株生长发育，提高碳、氮代谢水平，增加有效穗、穗粒数、穗实粒数，提高结实率、千粒重和晒干率等。处理比对照平均增产干谷0.964 t/hm2，增产率为15.4 %，并能改善品质。据多年多点多次的验收、考种和测产的数据表明，其增产效果稳定，重现性好。不仅产出/投入比高，而且具有无毒害、无污染、成本低、效益高和使用方法简便等优点，对增加粮食产量和发展无公害农业有重要意义。
《环境科学与工程前沿（英文）》 2023年 第17卷 第4期 doi: 10.1007/s11783-023-1643-y
● Higher concentrations of PS, PS-NH2 and PS-SO3H inhibited seed germination.
关键词： Nanoplastics Rape (Brassica napus L.) Physiology and biochemistry Nutrient absorption Plant quality Toxicity
《农业科学与工程前沿（英文）》 doi: 10.15302/J-FASE-2024573
● Developed a novel solar-powered corona dielectric barrier discharge (cDBD) microreactor for sustainable agriculture.
关键词： Non-thermal plasma plant growth reactor design seed germination
null
《农业科学与工程前沿（英文）》 2019年 第6卷 第4期 页码 357-365 doi: 10.15302/J-FASE-2019275
Phosphorus (P) is essential for life and for efficient crop production, but global P use with limited recycling is inefficient in several sectors, including agronomy. Unfortunately, plant physiologists, agronomists, farmers and end users employ different measures for P use efficiency (PUE), which often masks their values at different scales. The term P use effectiveness, which also considers energetic and sustainability measures in addition to P balances, is also a valuable concept. Major physiological and genetic factors for plant P uptake and utilization have been identified, but there has been limited success in genetically improving PUE of modern crop cultivars. In maize, studies on root architectural and morphological traits appear promising. Rhizosphere processes assist in mobilizing and capturing sparingly soluble phosphate from rock phosphate. Combinations of phosphate-solubilizing microorganisms with ammonium-based nitrogen fertilizer, as well as strategies of fertilizer placement near the roots of target crops, can moderately enhance PUE. The desired concentration of P in the products differs, depending on the final use of the crop products as feed, food or for energy conversion, which should be considered during crop production.
关键词： acquisition efficiency plant growth promoting rhizobacteria phosphate use efficiency utilization efficiency
Functional trait differences between native bunchgrasses and the invasive grass
Huiqin HE, Thomas A. MONACO, Thomas A. JONES
《农业科学与工程前沿（英文）》 2018年 第5卷 第1期 页码 139-147 doi: 10.15302/J-FASE-2017175
关键词： annual grass comparative growth drought response invasive plant native grass specific leaf area soil-water use
Optimizing hairy root production from explants of
Zhaogui YAN, Shengyu LIU, Junlian ZHANG, Guan HUANG, Lijun DUAN, Yaomei YE
《农业科学与工程前沿（英文）》 2020年 第7卷 第4期 页码 513-522 doi: 10.15302/J-FASE-2019272
is a shrub that has been used in traditional herbal medicine. It has great pharmaceutical potential for treating diseases such as cancer and diabetes. As a prerequisite for propagation of this species on a large scale, hairy roots in were induced using and various factors affecting hairy root induction and growth evaluated. Seven factors were tested: (1) type of explant, (2) type of culture medium, (3) duration of pre-culture, (4) inoculum cell density, (5) duration of infection, (6) acetosyringone concentration in the culture medium, and (7) duration of incubation. The optimal protocol for hairy root induction and growth was: young shoots, pre-cultured in Y1 for 2 d, inoculated with broth with an OD of 0.6 for 20 min, and incubated for 3 d. Putative transgenic hairy roots were initially identified by morphology and then confirmed by polymerase chain reaction. Successful and optimal production of hairy roots is a critical prerequisite for industrial scale clonal propagation of . Being able to cultivate the plant on a large scale will provide rapid and ready supply of the plant materials that can be used in herbal medicine and in scientific and industrial exploitation.
关键词： hairy root induction medicinal plant MS growth medium Phyllanthus hainanensis Rhizobium rhizogenes
Economic Growth Model Based on Six Basic Factors of Production—Xu Growth Rate Equation
Shou-bo Xu,Zong-yuan Huang
《工程管理前沿（英文）》 2014年 第1卷 第2期 页码 160-175 doi: 10.15302/J-FEM-2014024
关键词： six basic factors of production economic growth model Xu Growth Rate Equation
刘德盛,庄惠如,郑凌凌,林嵩,胡宁三
《中国工程科学》 2003年 第5卷 第12期 页码 12-18
螺旋藻是一种已有35亿年生命史的最古老的原核光合生物，已被联合国粮农组织（FAO）和世界卫生组织（WHO）誉为“21世纪最佳保健食品”。通过多年多点室内外试验研究，探明了TA乳粉促进螺旋藻优质高产的应用技术。在螺旋藻工厂化生产中，向养殖池添加TA0.01～0.02mg/L的TA乳粉，能促进螺旋藻的细胞分裂、增殖及藻丝生长，提高净光合速率和干物质积累，增强硝酸还原酶活力和呼吸强度，并显著提高蛋白质、氨基酸、藻蓝蛋白、β-胡萝卜素、叶绿素及类胡萝卜素等含量。在促进螺旋藻品质明显改善的同时，还能显著提高其生物产量，处理比对照的增产幅度可达1.19～1.76 g/d·m2，增产率20%～30%。用TA乳粉作为螺旋藻养殖的调节剂及其养殖工艺是一项新创举，并开拓了TA乳粉在微藻上应用研究的新领域，是促进螺旋藻优质高产的一种比较好的方法，应用前景十分广阔。
标题 作者 时间 类型 操作
Isolation, characterization and identification of plant growth-promoting rhizobacteria from the rhizosphere
期刊论文
Modeling water and heat transfer in soil-plant-atmosphere continuum applied to maize growth under plastic
Meng DUAN, Jin XIE, Xiaomin MAO
期刊论文
Combined use of inoculation and low phosphorus application increased plant growth, root nodulation and
期刊论文
Chromium phytoextraction and physiological responses of the hyperaccumulator Swartz to plant growth-promoting
期刊论文
Stress response to nanoplastics with different charges in L. during seed germination and seedling growth
期刊论文
Compact solar-powered plasma water generator: enhanced germination of aged seed with the corona dielectric barrier discharger
期刊论文
Improving the efficiency and effectiveness of global phosphorus use: focus on root and rhizosphere levels in the agronomic system
null
期刊论文
Functional trait differences between native bunchgrasses and the invasive grass
Huiqin HE, Thomas A. MONACO, Thomas A. JONES
期刊论文
Optimizing hairy root production from explants of
Zhaogui YAN, Shengyu LIU, Junlian ZHANG, Guan HUANG, Lijun DUAN, Yaomei YE
期刊论文
Economic Growth Model Based on Six Basic Factors of Production—Xu Growth Rate Equation
Shou-bo Xu,Zong-yuan Huang
期刊论文
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