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Mobile CRISPR-Cas9 based anti-phage system in E. coli
《化学科学与工程前沿(英文)》 2022年 第16卷 第8期 页码 1281-1289 doi: 10.1007/s11705-022-2141-7
关键词: phage infections anti-phage CRISPR-Cas9 conjugative transfer synthetic biology
Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG
《农业科学与工程前沿(英文)》 2015年 第2卷 第3期 页码 242-248 doi: 10.15302/J-FASE-2015059
CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells
null
《医学前沿(英文)》 2017年 第11卷 第4期 页码 554-562 doi: 10.1007/s11684-017-0543-6
T cells engineered with chimeric antigen receptor (CAR) have been successfully applied to treat advanced refractory B cell malignancy. However, many challenges remain in extending its application toward the treatment of solid tumors. The immunosuppressive nature of tumor microenvironment is considered one of the key factors limiting CAR-T efficacy. One negative regulator of T cell activity is lymphocyte activation gene-3 (LAG-3). We successfully generated LAG-3 knockout T and CAR-T cells with high efficiency using CRISPR-Cas9 mediated gene editing and found that the viability and immune phenotype were not dramatically changed during in vitro culture. LAG-3 knockout CAR-T cells displayed robust antigen-specific antitumor activity in cell culture and in murine xenograft model, which is comparable to standard CAR-T cells. Our study demonstrates an efficient approach to silence immune checkpoint in CAR-T cells via gene editing.
关键词: CAR-T CRISPR-Cas9 LAG-3
用于分枝杆菌碱基编辑的PAM扩展型嗜热链球菌Cas9 C到T和C到G碱基编辑器 Article
张洪源, 张翼飞, 王卫晓, 陈未中, 张侠, 黄行许, 陈伟, 季泉江
《工程(英文)》 2022年 第15卷 第8期 页码 67-77 doi: 10.1016/j.eng.2022.02.013
One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system
Hongbing HAN,Yonghe MA,Tao WANG,Ling LIAN,Xiuzhi TIAN,Rui HU,Shoulong DENG,Kongpan LI,Feng WANG,Ning LI,Guoshi LIU,Yaofeng ZHAO,Zhengxing LIAN
《农业科学与工程前沿(英文)》 2014年 第1卷 第1期 页码 2-5 doi: 10.15302/J-FASE-2014007
Genome-edited crops: how to move them from laboratory to market
Kunling CHEN, Caixia GAO
《农业科学与工程前沿(英文)》 2020年 第7卷 第2期 页码 181-187 doi: 10.15302/J-FASE-2020332
Recent breakthroughs in CRISPR technology allow specific genome manipulation of almost all crops and have initiated a revolution in precision crop breeding. Rationally-based regulation and widespread public acceptance are needed to propel genome-edited crops from laboratory to market and to translate this innovative technology into agricultural productivity.
关键词: CRISPR/Cas genome editing base editing precision breeding regulation
Embryo-mediated genome editing for accelerated genetic improvement of livestock
Zachariah MCLEAN, Björn OBACK, Götz LAIBLE
《农业科学与工程前沿(英文)》 2020年 第7卷 第2期 页码 148-160 doi: 10.15302/J-FASE-2019305
Selecting beneficial DNA variants is the main goal of animal breeding. However, this process is inherently inefficient because each animal only carries a fraction of all desirable variants. Genome editing technology with its ability to directly introduce beneficial sequence variants offers new opportunities to modernize animal breeding by overcoming this biological limitation and accelerating genetic gains. To realize rapid genetic gain, precise edits need to be introduced into genomically-selected embryos, which minimizes the genetic lag. However, embryo-mediated precision editing by homology-directed repair (HDR) mechanisms is currently an inefficient process that often produces mosaic embryos and greatly limits the numbers of available edited embryos. This review provides a summary of genome editing in bovine embryos and proposes an embryo-mediated accelerated breeding scheme that overcomes the present efficiency limitations of HDR editing in bovine embryos. It integrates embryo-based genomic selection with precise multi-editing and uses embryonic cloning with elite edited blastomeres or embryonic pluripotent stem cells to resolve mosaicism, enable multiplex editing and multiply rare elite genotypes. Such a breeding strategy would enable a more targeted, accelerated approach for livestock improvement that allows stacking of beneficial variants, even including novel traits from outside the breeding population, in the most recent elite genetic background, essentially within a single generation.
关键词: animal breeding cattle cloning CRISPR/Cas9 cytoplasmic injection embryo genome editing germline chimaeras HDR livestock improvement TALENs
Jennifer Welsh
《工程(英文)》 2023年 第30卷 第11期 页码 3-6 doi: 10.1016/j.eng.2023.09.005
is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice
Yuqin Men, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun, Haibo Wang, Jiangang Gao
《医学前沿(英文)》 2019年 第13卷 第6期 页码 690-704 doi: 10.1007/s11684-018-0638-8
关键词: TPRN stereocilia stereociliary rootlet actin filament CRISPR/Cas9 hearing
Sarah C.P. Williams
《工程(英文)》 2023年 第20卷 第1期 页码 6-8 doi: 10.1016/j.eng.2022.11.002
利用基因组特异性引物和Cas12a介导技术快速检测稻瘟菌小麦致病型 Article
康厚祥, 彭烨, Kangyu Hua, 邓雨飞, Maria Bellizzi, Dipali Rani Gupta, Nur Uddin Mahmud, Alfredo S. Urashima, Sanjoy Kumar Paul, Gary Peterson, 周益林, 周雪平, Md Tofazzal Islam, 王国梁
《工程(英文)》 2021年 第7卷 第9期 页码 1326-1335 doi: 10.1016/j.eng.2020.07.016
Genome reprogramming for synthetic biology
Kylie Standage-Beier,Xiao Wang
《化学科学与工程前沿(英文)》 2017年 第11卷 第1期 页码 37-45 doi: 10.1007/s11705-017-1618-2
关键词: CRISPR genome engineering synthetic biology rational design
具有异构时延的多智能体系统的领导-跟随同步 Research Articles
Branislav REH罧, Volodymyr LYNNYK
《信息与电子工程前沿(英文)》 2021年 第22卷 第1期 页码 1-140 doi: 10.1631/FITEE.2000207
关键词: 多智能体系统;时滞;线性矩阵不等式
A PLATFORM TO AID SELECT THE OPTIMAL TOOL TO DESIGN GUIDE RNAS
《农业科学与工程前沿(英文)》 页码 296-305 doi: 10.15302/J-FASE-2022479
● Summaries on sgRNAs design.
标题 作者 时间 类型 操作
Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330
Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG
期刊论文
One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system
Hongbing HAN,Yonghe MA,Tao WANG,Ling LIAN,Xiuzhi TIAN,Rui HU,Shoulong DENG,Kongpan LI,Feng WANG,Ning LI,Guoshi LIU,Yaofeng ZHAO,Zhengxing LIAN
期刊论文
Embryo-mediated genome editing for accelerated genetic improvement of livestock
Zachariah MCLEAN, Björn OBACK, Götz LAIBLE
期刊论文
is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice
Yuqin Men, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun, Haibo Wang, Jiangang Gao
期刊论文
利用基因组特异性引物和Cas12a介导技术快速检测稻瘟菌小麦致病型
康厚祥, 彭烨, Kangyu Hua, 邓雨飞, Maria Bellizzi, Dipali Rani Gupta, Nur Uddin Mahmud, Alfredo S. Urashima, Sanjoy Kumar Paul, Gary Peterson, 周益林, 周雪平, Md Tofazzal Islam, 王国梁
期刊论文