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期刊论文 5

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2023 1

2022 1

2018 2

2016 1

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含油污泥 1

烃降解产甲烷 1

烷烃 1

生物修复 1

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Developing “precise-acting” strategies for improving anaerobic methanogenesis of organic waste: Insights

《环境科学与工程前沿(英文)》 2022年 第16卷 第6期 doi: 10.1007/s11783-021-1508-1

摘要: Methanogenesis is the last step in anaerobic digestion, which is usually a rate-limiting step in the biological treatment of organic waste. The low methanogenesis efficiency (low methane production rate, low methane yield, low methane content) substantially limits the development of anaerobic digestion technology. Traditional pretreatment methods and bio-stimulation strategies have impacts on the entire anaerobic system and cannot directly enhance methanogenesis in a targeted manner, which was defined as “broad-acting” strategies in this perspective. Further, we discussed our opinion of methanogenesis process with insights from the electron transfer system of syntrophic partners and provided potential targeted enhancing strategy for high-efficiency electron transfer system. These “precise-acting” strategies are expected to achieve an efficient methanogenesis process and enhance the bio-energy recovery of organic waste.

关键词: Methanogenesis     Anaerobic digestion     Enhancing strategy     Electron transfer     Organic waste    

Methanogenic community structure in simultaneous methanogenesis and denitrification granular sludge

Yujiao Sun, Juanjuan Zhao, Lili Chen, Yueqiao Liu, Jiane Zuo

《环境科学与工程前沿(英文)》 2018年 第12卷 第4期 doi: 10.1007/s11783-018-1067-2

摘要:

• UASB with SMD granules was operated with high removal efficiency of COD and NO3--N.

Methanosaetaceae was absolute predominant methanogen in SMD granules.

• The methanogen quantity and activity decreased as C/N decreased from 20:1 to 5:1.

• Bacterial community succession happened with C/N decreasing.

关键词: Simultaneous methanogenesis and denitrification (SMD)     Methanogens     Community structure     Diversity index     Granular sludge    

Influence of arsanilic acid, Cu

Ping He, Guangxue Wu, Rui Tang, Peilun Ji, Shoujun Yuan, Wei Wang, Zhenhu Hu

《环境科学与工程前沿(英文)》 2018年 第12卷 第2期 doi: 10.1007/s11783-017-1004-9

摘要: Arsanilic acid (ASA), copper ion (Cu ) and phosphate (PO ) are widely used as feed additives for pigs. Most of these three supplemented feed additives were excreted in feces and urine. Anaerobic digestion is often used for the management of pig manure. However, the interaction of ASA with Cu or PO on anaerobic digestion is still not clear. In this study, the influence of ASA, Cu , PO and their interaction on anaerobic digestion of pig manure and the possible mechanisms were investigated. The initial concentrations of ASA, Cu and PO were 0.46 mM, 2 mM and 2 mM in the anaerobic digester, respectively. The methanogenesis was severely inhibited in the assays with only ASA addition, only Cu addition and ASA+ PO addition with the inhibition index of 97.8%, 46.6% and 82.6%, respectively, but the methanogenesis inhibition in the assay with ASA+ Cu addition was mitigated with the inhibition index of 39.4%. PO had no obvious impacts on the degradation of ASA. However, Cu addition inhibited the degradation of ASA, mitigating the methanogenesis inhibition. The existence of ASA would inhibit methanogenesis and generate more toxic inorganic arsenic compounds during anaerobic digestion, implying the limitation of anaerobic digestion for ASA- contaminated animal manure. However, the co-existence of ASA and Cu could mitigate the inhibition. These results could provide useful information for the management of anaerobic digestion of pig manure containing ASA with Cu .

关键词: Arsanilic acid (ASA)     Methanogenesis     Inhibition     Copper     Phosphate     Inorganic arsenics    

A syntrophic propionate-oxidizing microflora and its bioaugmentation on anaerobic wastewater treatment for enhancing methane production and COD removal

Chong Liu, Jianzheng Li, Shuo Wang, Loring Nies

《环境科学与工程前沿(英文)》 2016年 第10卷 第4期 doi: 10.1007/s11783-016-0856-8

摘要: Syntrophic propionate-oxidizing microflora B83 was enriched from anaerobic sludge. The bioaugmentation of microflora B83 were evaluated from wastewater treatment. Methane yield and COD removal were enhanced by bioaugmentation of microflora B83. Hydrogen-producing acetogensis was a rate-limiting step in methane fermentation. Methane fermentation process can be restricted and even destroyed by the accumulation of propionate because it is the most difficult to be anaerobically oxidized among the volatile fatty acids produced by acetogenesis. To enhance anaerobic wastewater treatment process for methane production and COD removal, a syntrophic propionate-oxidizing microflora B83 was obtained from an anaerobic activated sludge by enrichment with propionate. The inoculation of microflora B83, with a 1:9 ratio of bacteria number to that of the activated sludge, could enhance the methane production from glucose by 2.5 times. With the same inoculation dosage of the microflora B83, COD removal in organic wastewater treatment process was improved from 75.6% to 86.6%, while the specific methane production by COD removal was increased by 2.7 times. Hydrogen-producing acetogenesis appeared to be a rate-limiting step in methane fermentation, and the enhancement of hydrogen-producing acetogens in the anaerobic wastewater treatment process had improved not only the hydrogen-producing acetogenesis but also the acidogenesis and methanogenesis.

关键词: Anaerobic wastewater treatment     Methane production     Hydrogen-producing acetogenesis     Methanogenesis     Rate-limiting step     Bioaugmentation    

长期培养和宏组学分析揭示了烃污染环境中的甲基营养型产甲烷途径 Article

刘一凡, 陈静, 刘忠林, 侯兆伟, 梁波, 王立影, 周蕾, 寿利斌, 林丹丹, 杨世忠, 刘金峰, 伍晓林, 顾继东, 牟伯中

《工程(英文)》 2023年 第24卷 第5期   页码 265-276 doi: 10.1016/j.eng.2021.08.027

摘要:

针对石油烃污染的厌氧环境, 利用微生物的代谢作用将石油烃转化为甲烷是一种潜在的生物修复策略。然而, 目前对于微生物厌氧烃降解产甲烷的机制尚不清楚。经过十年的持续富集和转接培养, 我们获得了一种能够降解正构烷烃 (C15~C20) 并且产甲烷富集培养体系, 并且经过转接培养该培养物在甲烷生产的动力学特性方面得到了持续的提高。综合宏基因组和宏转录组的分析结果, 发现正构烷烃主要通过DesulfosarcinaceaeFirmicutesSynergistetes菌通过富马酸加成的方式进行起始活化, 然后在Tepidiphilus菌的协同作用下进一步降解。同时, 体系中含量较多的Anaerolineaceae菌主要负责死细胞生物质的回收。根据宏转录组学分析结果, 甲烷主要通过H2依赖型的甲基营养甲烷途径产生, 主要由候选门Verstraetearchaeta内的Methanomethyliaceae通过利用死细胞生物质回收代谢过程中产生的三甲胺来产生甲烷。这些发现表明, 依赖H2的甲基营养产甲烷菌以及甲基营养产甲烷菌可能在含石油烃的地下生态系统的碳循环过程中发挥着重要的作用。

关键词: 烃降解产甲烷     含油污泥     生物修复     烷烃    

标题 作者 时间 类型 操作

Developing “precise-acting” strategies for improving anaerobic methanogenesis of organic waste: Insights

期刊论文

Methanogenic community structure in simultaneous methanogenesis and denitrification granular sludge

Yujiao Sun, Juanjuan Zhao, Lili Chen, Yueqiao Liu, Jiane Zuo

期刊论文

Influence of arsanilic acid, Cu

Ping He, Guangxue Wu, Rui Tang, Peilun Ji, Shoujun Yuan, Wei Wang, Zhenhu Hu

期刊论文

A syntrophic propionate-oxidizing microflora and its bioaugmentation on anaerobic wastewater treatment for enhancing methane production and COD removal

Chong Liu, Jianzheng Li, Shuo Wang, Loring Nies

期刊论文

长期培养和宏组学分析揭示了烃污染环境中的甲基营养型产甲烷途径

刘一凡, 陈静, 刘忠林, 侯兆伟, 梁波, 王立影, 周蕾, 寿利斌, 林丹丹, 杨世忠, 刘金峰, 伍晓林, 顾继东, 牟伯中

期刊论文