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The impact of different voltage application modes on biodegradation of chloramphenicol and shift of microbialcommunity structure

《环境科学与工程前沿（英文）》 2022年 第16卷 第11期 doi: 10.1007/s11783-022-1576-x

摘要：

● Presented coupled system enhanced biodegradation of antibiotic chloramphenicol.

关键词： Electrical stimulation     Biodegradation     Microbial community     Chloramphenicol    

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Influence of pore structure on biologically activated carbon performance and biofilm microbial characteristics

《环境科学与工程前沿（英文）》 2021年 第15卷 第6期 doi: 10.1007/s11783-021-1419-1

摘要：

• Pore structure affects biologically activated carbon performance.

关键词： Granular activated carbon     Biologically activated carbon filter     Bacterial community structure     Pore structure    

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Effect of the ultraviolet/chlorine process on microbial community structure, typical pathogens, and antibiotic

《环境科学与工程前沿（英文）》 2022年 第16卷 第8期 doi: 10.1007/s11783-022-1521-z

摘要：

• UV/chlorine can effectively remove VBNC pathogens, ARGs and MGEs in reclaimed water.

关键词： UV/chlorine process     Pathogen     Antibiotic resistance genes     High-throughput qPCR     Reclaimed water    

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Identifying human-induced influence on microbial community: A comparative study in the effluent-receiving

Yuhan Zheng, Zhiguo Su, Tianjiao Dai, Feifei Li, Bei Huang, Qinglin Mu, Chuanping Feng, Donghui Wen

《环境科学与工程前沿（英文）》 2019年 第13卷 第6期 doi: 10.1007/s11783-019-1174-8

摘要： Microbial compositions showed high differences in two study areas. COD was the key anthropogenic indicator in the coastal wastewater disposal area. Distinctive microbes capable of degrading toxic pollutants were screened. Microbial communities in effluent-receiving areas followed “niche theory”. Microbial community structure is affected by both natural processes and human activities. In coastal area, anthropegenetic activity can usually lead to the discharge of the effluent from wastewater treatment plant (WWTP) to sea, and thus the water quality chronically turns worse and marine ecosystem becomes unhealthy. Microorganisms play key roles in pollutants degradation and ecological restoration; however, there are few studies about how the WWTP effluent disposal influences coastal microbial communities. In this study, sediment samples were collected from two WWTP effluent-receiving areas (abbreviated as JX and SY) in Hangzhou Bay. First, based on the high-throughput sequencing of 16S rRNA gene, microbial community structure was analyzed. Secondly, several statistical analyses were conducted to reveal the microbial community characteristics in response to the effluent disposal. Using PCoA, the significant difference of in microbial community structure was determined between JX and SY; using RDA, water COD and temperature, and sediment available phosphate and ammonia nitrogen were identified as the key environmental factors for the community difference; using LDA effect size analysis, the most distinctive microbes were found and their correlations with environmental factors were investigated; and according to detrended beta-nearest-taxon-index, the sediment microbial communities were found to follow “niche theory”. An interesting and important finding was that in SY that received more and toxic COD, many distinctive microbes were related to the groups that were capable of degrading toxic organic pollutants. This study provides a clear illustration of eco-environmental deterioration under the long-term human pressure from the view of microbial ecology.

关键词： Microbial community structure     Effluent-receiving area     High-throughput sequencing     Costal sediments     Wastewater treatment plant (WWTP)    

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Microbial community structure in different wastewater treatment processes characterized by single-strand

ZHAO Yangguo, WANG Aijie, REN Nanqi, ZHAO Yan

《环境科学与工程前沿（英文）》 2008年 第2卷 第1期   页码 116-121 doi: 10.1007/s11783-008-0018-8

摘要： In order to investigate microbial community structures in different wastewater treatment processes and understand the relationship between the structures and the status of processes, the microbial community diversity, variety and distribution in five wastewater treatment pro cesses were studied by a culture-independent genetic fingerprinting technique single-strand conformation polymorphism (SSCP). The five processes included denitrifying and phosphate-removal system (diminished N), Chinese traditional medicine wastewater treatment system (P), beer wastewater treatment system (W), fermentative biohydrogen-producing system (H), and sulfate-reduction system (S). The results indicated that the microbial community profiles in the wastewater bioreactors with the uniform status were very similar. The diversity of microbial populations was correlated with the complexity of organic contaminants in wastewater. Chinese traditional medicine wastewater contained more complex organic components; hence, the population diversity was higher than that of simple nutrient bioreactors fed with molasses wastewater. Compared with the strain bands in a simulated community, the relative proportion of some functional microbial populations in bioreactors was not dominant. Fermentative biohydrogen producer in the better condition bioreactor had only a 5% band density, and the sp. in the sulfate-reducing bioreactor had less than 1.5% band density. The SSCP profiles could identify the difference in microbial community structures in wastewater treatment processes, monitor some of the functional microbes in these processes, and consequently provide useful guidance for improving their efficiency.

关键词： technique single-strand     proportion     bioreactor     community diversity     fingerprinting technique    

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Microbial community structure and dynamics of starch-fed and glucose-fed chemostats during two years

Min GOU,Jing ZENG,Huizhong WANG,Yueqin TANG,Toru SHIGEMATSU,Shigeru MORIMURA,Kenji KIDA

《环境科学与工程前沿（英文）》 2016年 第10卷 第2期   页码 368-380 doi: 10.1007/s11783-015-0815-9

摘要： The microbial community structures of two mesophilic anaerobic chemostats, one fed with glucose, the other with starch as sole carbon sources, were studied at various dilution rates (0.05–0.25 d for glucose and 0.025–0.1 d for starch) during two years continuous operation. In the glucose-fed chemostat, the aceticlastic methanogen spp. and hydrogenotrophic methanogen spp. predominated at low dilution rates, whereas spp. and the hydrogenotrophic spp. predominated together when dilution rates were greater than 0.1 d . Bacteria affiliated with the phyla Bacteroidetes, Spirochaetes, and Actinobacteria predominated at dilution rates of 0.05, 0.1, and 0.15 d , respectively, while Firmicutes predominated at higher dilution rates (0.2 and 0.25 d ). In the starch-fed chemostat, the aceticlastic and hydrogenotrophic methanogens coexisted at all dilution rates. Although bacteria belonging to only two phyla were mainly responsible for starch degradation (Spirochaetes at the dilution rate of 0.08 d and Firmicutes at other dilution rates), different bacterial genera were identified at different dilution rates. With the exception of Archaea in the glucose-fed chemostat, the band patterns revealed by denaturing gradient gel electrophoresis (DGGE) of the microbial communities in the two chemostats displayed marked changes during long-term operation at a constant dilution rate. The bacterial community changed with changes in the dilution rate, and was erratic during long-term operation in both glucose-fed and starch-fed chemostats.

关键词： microbial community     glucose degradation     starch degradation     dilution rate     continuous methane fermentation     phylogenetic analysis    

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Microbial community dynamics at high organic loading rates revealed by pyrosequencing during sugar refinery

Liguo Zhang, Qiaoying Ban, Jianzheng Li

《环境科学与工程前沿（英文）》 2018年 第12卷 第4期 doi: 10.1007/s11783-018-1045-8

摘要：

High strength sugar refinery wastewater was treated in a mesophilic UASB.

Pyrosequencing reveals microbial community succession with OLR increase.

Diversity of microbial communities in OLR12 is much higher than those in OLR36 and OLR54.0 kgCOD/(kg VSS·d).

Fermentative bacteria could deal with increasing OLR through the increase of microbial diversity and quantity.

Hydrogen-producing acotogens and methanogens mainly coped with high OLR shocks by increasing the quantity of community

关键词： Upflow anaerobic sludge blanket     Sugar refinery wastewater     Organic loading rate     Pyrosequencing     Microbial community structure    

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Insights into the changes of amino acids, microbial community, and enzymatic activities related with

《环境科学与工程前沿（英文）》 2023年 第17卷 第3期 doi: 10.1007/s11783-023-1635-y

摘要：

● The highest seed germination index was achieved at 0.3 g/g total solids of food waste.

关键词： Composting     Food waste     Amino acids     Microbial community     Enzymatic activity     Nutrient quality    

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Resistance to salt stresses by aerobic granular sludge: sludge property and microbial community

《环境科学与工程前沿（英文）》 2024年 第18卷 第8期 doi: 10.1007/s11783-024-1861-y

摘要：

● Aerobic granular sludge could withstand long-term saline stresses.

关键词： Aerobic granular sludge     Long-term saline stresses     Performance     Sludge property     Microbial community    

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Responses of microbial interactions to elevated salinity in activated sludge microbial community

《环境科学与工程前沿（英文）》 2023年 第17卷 第5期 doi: 10.1007/s11783-023-1660-x

摘要：

● Salinity led to the elevation of NAR over 99.72%.

关键词： Elevated salinity     Activated sludge system     Pollution removal     Microbial interactions     Competitive relationship    

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Effect of rhizosphere on soil microbial community and pyrene biodegradation

SU Yuhong, YANG Xueyun, CHIOU Cary

《环境科学与工程前沿（英文）》 2008年 第2卷 第4期   页码 468-474 doi: 10.1007/s11783-008-0078-9

摘要： To access the influence of a vegetation on soil microorganisms toward organic pollutant biogegration, this study examined the rhizospheric effects of four plant species (sudan grass, white clover, alfalfa, and fescue) on the soil microbial community and pyrene (PYR) biodegradation. The results indicated that the spiked PYR levels in soils decreased substantially compared to the control soil without planting. With equal planted densities, the efficiencies of PYR degradation in rhizosphere with sudan grass, white clover, alfalfa and fescue were 34.0%, 28.4%, 27.7%, and 9.9%, respectively. However, on the basis of equal root biomass the efficiencies were in order of white clover >> alfalfa > sudan > fescue. The increased PYR biodegradation was attributed to the enhanced bacterial population and activity induced by plant roots in the rhizosphere. Soil microbial species and biomasses were elucidated in terms of microbial phospholipid ester-linked fatty acid (PLFA) biomarkers. The principal component analysis (PCA) revealed significant changes in PLFA pattern in planted and non-planted soils spiked with PYR. Total PLFAs in planted soils were all higher than those in non-planted soils. PLFA assemblages indicated that bacteria were the primary PYR degrading microorganisms, and that Gram-positive bacteria exhibited higher tolerance to PYR than Gram-negative bacteria did.
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Changes of microbial composition during wastewater reclamation and distribution systems revealed by high-throughput

Yiwen LIN,Dan LI,Siyu ZENG,Miao HE

《环境科学与工程前沿（英文）》 2016年 第10卷 第3期   页码 539-547 doi: 10.1007/s11783-016-0830-5

摘要： This study employed 454-pyrosequencing to investigate microbial and pathogenic communities in two wastewater reclamation and distribution systems. A total of 11972 effective 16S rRNA sequences were acquired from these two reclamation systems, and then designated to relevant taxonomic ranks by using RDP classifier. The Chao index and Shannon diversity index showed that the diversities of microbial communities decreased along wastewater reclamation processes. was the most dominant phylum in reclaimed water after disinfection, which accounted for 83% and 88% in two systems, respectively. Human opportunistic pathogens, including , , and , were selected and enriched by disinfection processes. The total chlorine and nutrients (TOC, NH -N and NO -N) significantly affected the microbial and pathogenic communities during reclaimed water storage and distribution processes. Our results indicated that the disinfectant-resistant pathogens should be controlled in reclaimed water, since the increases in relative abundances of pathogenic bacteria after disinfection implicate the potential public health associated with reclaimed water.

关键词： wastewater reclamation systems     microbial community     pathogenic community     454-pyrosequencing    

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Comparative study on microbial community in intermittently aerated sequencing batch reactors (SBR) and

Xiaolin Sheng, Rui Liu, Xiaoyan Song, Lujun Chen, Kawagishi Tomoki

《环境科学与工程前沿（英文）》 2017年 第11卷 第3期 doi: 10.1007/s11783-017-0929-3

摘要： A traditional sequencing batch reactor (SBR) and two intermittently aerated sequencing batch reactors (IASBRs) were parallelly operated for treating digested piggery wastewater. Their microbial communities were analyzed, and the nitrogen removal performance was compared during the long–term run. IASBRs demonstrated higher removal rates of total nitrogen (TN) and ammonium nitrogen (NH -N) than the SBR, and also demonstrated higher resistance against TN shock load. It was found that the more switch times between aerobic/anoxic in an IASBR, the higher the removal rates of TN and NH –N. All the reactors were predominated by , and , which were considered to be species of denitrifiers, ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB), respectively. However, the abundance and diversity was of great difference. Compared with SBR, IASBRs achieved higher abundance of denitrification–related bacteria. IASBR 1 with four aerobic/anoxic switch times was detected with 25.63% of , higher than that in IASBR 2 with two aerobic/anoxic switch times (11.57% of ), and much higher than that in the SBR (only 6.19% of ). IASBR 2 had the highest percentage of AOB, while IASBR 1 had the lowest percentage. The denitrifiers abundance was significantly positive correlated with the TN removal rate. However, the NH –N removal rate showed no significant correlation with the AOB abundance, but might relate to the AOB activity which was influenced by the average free ammonium (FA) concentration. was the only NOB genus detectable in all reactors, and were less than 0.03%.

关键词： Digested piggery wastewater     Intermittent aeration     Microbial community     Partial nitrification–denitrification process     Sequencing batch reactor (SBR)    

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Biochar-compost-based controlled-release nitrogen fertilizer intended for an active microbial community RESEARCH ARTICLE

《农业科学与工程前沿（英文）》 2024年 第11卷 第2期   页码 326-343 doi: 10.15302/J-FASE-2024571

摘要：

Nitrogen (N) fertilizers in agriculture suffer losses by volatilization of N to the air, surface runoff and leaching into the soil, resulting in low N use efficiency (NUE) (\lt50%) and raising severe environmental pollutions. Controlled-release nitrogen fertilizers (CRNFs) can control the release of N nutrients to NUE in crop production. Different methods were used to develop new CRNFs. However, different CRNF technologies are still underdeveloped due to inadequate controlling on N releasing time and/or unsustainable diffusion. The study on the influences of CRNF processing parameters on microbial conditions are lacking when the CRNFs composed of various bio-ingredients such as biochar, composts, and biowaste. The complexity of processing methods, material biodegradability, and other physical properties make current CRNFs of questionable value in agricultural production. This research aims to develop a novel biochar-compost-based controlled-release urea fertilizer (BCRUF) to preserve microbial properties carried by the compost. The BCRUF was synthesized by pelletizing the 50:50 (dry, wt/wt) mixture of biochar and compost. BCRUF was loaded with urea and then spray-coated with polylactic acid (PLA). The releasing time of two types of BCRUFs, coated and uncoated with PLA, for 80% of N release in water was up to 6 h at three different temperatures (4, 23, and 40 °C), compared to conventional urea fertilizer and commercial environmentally smart N (ESN) fertilizer. The releasing time of coated BCRUF for 80% N release in soil was up to 192 h (8 days). Fourier-transform infrared spectroscopy (FTIR) analysis revealed that no new functional groups were found in the release solution, indicating no new chemical hazards generated. The differential scanning calorimetry (DSC) tests also verified that its thermal stability could be up to 160 °C. The microbe populations in the BCRUF pellets were reduced after the pelleting and drying processes in BCRUF fabrication, but a few bacteria can endure in the air-drying process. BCRUF pellets soaked in water for 4 days retained some bacteria. The BCRUF showed very promising characteristics to improve NUE and sustainability in agricultural production.

关键词： Soil microbial community     biochar     compost     controlled-release nitrogen fertilizer     polylactic acid     spray coating.    

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thermophilic anaerobic digestion of swine manure with sulfamethoxazole and norfloxacin: Dynamics of microbial

《环境科学与工程前沿（英文）》 2021年 第15卷 第5期 doi: 10.1007/s11783-020-1342-x

摘要：

• SMX addition had negative effect on acetoclastic methanogens in mesophilic AD.

关键词： Pig manure     Antibiotics     Anaerobic digestion     Resistance genes     Microbial community    

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标题 作者 时间 类型 操作

The impact of different voltage application modes on biodegradation of chloramphenicol and shift of microbialcommunity structure

期刊论文

Influence of pore structure on biologically activated carbon performance and biofilm microbial characteristics

期刊论文

Effect of the ultraviolet/chlorine process on microbial community structure, typical pathogens, and antibiotic

期刊论文

Identifying human-induced influence on microbial community: A comparative study in the effluent-receiving

Yuhan Zheng, Zhiguo Su, Tianjiao Dai, Feifei Li, Bei Huang, Qinglin Mu, Chuanping Feng, Donghui Wen

期刊论文

Microbial community structure in different wastewater treatment processes characterized by single-strand

ZHAO Yangguo, WANG Aijie, REN Nanqi, ZHAO Yan

期刊论文

Microbial community structure and dynamics of starch-fed and glucose-fed chemostats during two years

Min GOU,Jing ZENG,Huizhong WANG,Yueqin TANG,Toru SHIGEMATSU,Shigeru MORIMURA,Kenji KIDA

期刊论文

Microbial community dynamics at high organic loading rates revealed by pyrosequencing during sugar refinery

Liguo Zhang, Qiaoying Ban, Jianzheng Li

期刊论文

Insights into the changes of amino acids, microbial community, and enzymatic activities related with

期刊论文

Resistance to salt stresses by aerobic granular sludge: sludge property and microbial community

期刊论文

Responses of microbial interactions to elevated salinity in activated sludge microbial community

期刊论文

Effect of rhizosphere on soil microbial community and pyrene biodegradation

SU Yuhong, YANG Xueyun, CHIOU Cary

期刊论文

Changes of microbial composition during wastewater reclamation and distribution systems revealed by high-throughput

Yiwen LIN,Dan LI,Siyu ZENG,Miao HE

期刊论文

Comparative study on microbial community in intermittently aerated sequencing batch reactors (SBR) and

Xiaolin Sheng, Rui Liu, Xiaoyan Song, Lujun Chen, Kawagishi Tomoki

期刊论文

Biochar-compost-based controlled-release nitrogen fertilizer intended for an active microbial community

期刊论文

thermophilic anaerobic digestion of swine manure with sulfamethoxazole and norfloxacin: Dynamics of microbial

期刊论文