检索范围:
排序: 展示方式:
Using pyrosequencing and quantitative PCR to analyze microbial communities
Husen ZHANG
《环境科学与工程前沿(英文)》 2011年 第5卷 第1期 页码 21-27 doi: 10.1007/s11783-011-0303-9
关键词: polymerase chain reaction (PCR) microbial communities pyrosequencing gut microbial fuel cell sludge
李现明,张玉林,李建田
《中国工程科学》 2007年 第9卷 第7期 页码 76-81
设计了一种用于聚合酶链式反应的低成本微反应器,其内部无微加热器、微传感器。针对这种低成本的微反应器,提出了一种温度测量与控制方案,其特点是将各反应器温度的集中控制与分散控制有机结合,能够同时完成上百只微反应器快速、准确的温度循环。重点阐述了温度传感器负荷效应的提取与补偿、动态误差补偿、微反应器温度间接测量误差评定、微反应器温度串级控制策略。
Erratum to: Using pyrosequencing and quantitative PCR to analyze microbial communities
Husen ZHANG
《环境科学与工程前沿(英文)》 2011年 第5卷 第3期 页码 488-488 doi: 10.1007/s11783-011-0342-2
Safety evaluation of microbial pesticide (HaNPV) based on PCR method
Miao Zhao, Shufei Li, Qinghong Zhou, Dianming Zhou, Ning He, Zhiyong Qian
《化学科学与工程前沿(英文)》 2019年 第13卷 第2期 页码 377-384 doi: 10.1007/s11705-018-1777-9
关键词: microbial pesticides HaNPV acute intravenous injection infectivity pathogenicity
Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,
《医学前沿(英文)》 2010年 第4卷 第2期 页码 204-207 doi: 10.1007/s11684-010-0043-4
关键词: Angiostrongylus cantonensis Pomacea canaliculata multiplex polymerase chain reaction diagnosis pooling
彭年才,李 磊,李 政,苗保刚,李 明,赵 垚,蒋庄德
《中国工程科学》 2013年 第15卷 第1期 页码 57-62
以生物检测与分子诊断技术发展为背景,总结了团队在医学诊断、疾控、食品安全、检疫检验、生物学医学科学研究等领域的分子诊断高端装备的研制情况,重点介绍了实时荧光定量聚合酶链式反应(PCR)分子诊断仪关键技术的研发及仪器工程化技术
基于抗原和聚合酶链式反应的组合策略对模拟社区内遏制COVID-19传播效果的定量分析 Article
黄蔷如, 孙艳侠, 贾萌萌, 张婷, 陈方圆, 姜明月, 王晴, 冯录召, 杨维中
《工程(英文)》 2023年 第28卷 第9期 页码 234-242 doi: 10.1016/j.eng.2023.01.004
Kun DING,Xianghua WEN,Liang CHEN,Daishi HUANG,Fan FEI,Yuyang LI
《环境科学与工程前沿(英文)》 2014年 第8卷 第5期 页码 693-702 doi: 10.1007/s11783-014-0635-3
关键词: ammonia-oxidizing archaea ammonia-oxidizing bacteria quantitative PCR clone library plateau
Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO
《环境科学与工程前沿(英文)》 2011年 第5卷 第2期 页码 175-185 doi: 10.1007/s11783-011-0320-8
关键词: quantitative polymerase chain reaction (PCR) denaturing gradient gel electrophoresis (DGGE) principal component analysis (PCA) canonical correspondence analysis (CCA)
Assessment of antibiotic resistance genes in dialysis water treatment processes
Xuan Zhu, Chengsong Ye, Yuxin Wang, Lihua Chen, Lin Feng
《环境科学与工程前沿(英文)》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1136-1
• Quantitative global ARGs profile in dialysis water was investigated. • Totally 35 ARGs were found in the dialysis treatment train. • 29 ARGs (highest) were found in carbon filtration effluent. • erm and mtrD-02 occurred in the final effluent. • The effluent was associated with health risks even after RO treatment.
关键词: Dialysis water Treatment process Antibiotic resistance gene High-throughput quantitative PCR Horizontal gene transfer
Seasonal microbial community shift in a saline sewage treatment plant
Qingmei YAN, Xuxiang ZHANG, Tong ZHANG, Herbert H P FANG
《环境科学与工程前沿(英文)》 2011年 第5卷 第1期 页码 40-47 doi: 10.1007/s11783-011-0304-8
关键词: sewage treatment plants polymerase chain reaction (PCR) denaturing gradient gel electrophoresis (DGGE) canonical correspondence analysis correspondence analysis
Nucleic acid-based diagnostics for infectious diseases in public health affairs
null
《医学前沿(英文)》 2012年 第6卷 第2期 页码 173-186 doi: 10.1007/s11684-012-0195-5
Infectious diseases, mostly caused by bacteria and viruses but also a result of fungal and parasitic infection, have been one of the most important public health concerns throughout human history. The first step in combating these pathogens is to get a timely and accurate diagnosis at an affordable cost. Many kinds of diagnostics have been developed, such as pathogen culture, biochemical tests and serological tests, to help detect and fight against the causative agents of diseases. However, these diagnostic tests are generally unsatisfactory because they are not particularly sensitive and specific and are unable to deliver speedy results. Nucleic acid-based diagnostics, detecting pathogens through the identification of their genomic sequences, have shown promise to overcome the above limitations and become more widely adopted in clinical tests. Here we review some of the most popular nucleic acid-based diagnostics and focus on their adaptability and applicability to routine clinical usage. We also compare and contrast the characteristics of different types of nucleic acid-based diagnostics.
关键词: nucleic acid-based diagnostics infectious disease PCR NASBA LAMP microarray LOAC public health affairs
662 A/G gene variation in human tumor necrosis factor receptor superfamily, member 9 (TNFRSF9)
QU Yanchun, YANG Ze, SUN Liang, JI Linong
《医学前沿(英文)》 2008年 第2卷 第3期 页码 283-285 doi: 10.1007/s11684-008-0053-7
关键词: D-HPLC mutation development autoimmune PCR-RFLP candidate
null
《医学前沿(英文)》 2018年 第12卷 第2期 页码 196-205 doi: 10.1007/s11684-017-0560-5
We employed a multiplex polymerase chain reaction (PCR) coupled with capillary electrophoresis (mPCR-CE) targeting six Clostridium difficile genes, including tpi, tcdA, tcdB, cdtA, cdtB, and a deletion in tcdC for simultaneous detection and characterization of toxigenic C. difficile directly from fecal specimens. The mPCR-CE had a limit of detection of 10 colony-forming units per reaction with no cross-reactions with other related bacterial genes. Clinical validation was performed on 354 consecutively collected stool specimens from patients with suspected C. difficile infection and 45 isolates. The results were compared with a reference standard combined with BD MAX Cdiff, real-time cell analysis assay (RTCA), and mPCR-CE. The toxigenic C. difficile species were detected in 36 isolates and 45 stool specimens by the mPCR-CE, which provided a positive rate of 20.3% (81/399). The mPCR-CE had a specificity of 97.2% and a sensitivity of 96.0%, which was higher than RTCA (x2 = 5.67, P = 0.017) but lower than BD MAX Cdiff (P = 0.245). Among the 45 strains, 44 (97.8%) were determined as non-ribotype 027 by the mPCR-CE, which was fully agreed with PCR ribotyping. Even though ribotypes 017 (n = 8, 17.8%), 001 (n = 6, 13.3%), and 012 (n = 7, 15.6%) were predominant in this region, ribotype 027 was an important genotype monitored routinely. The mPCR-CE provided an alternative diagnosis tool for the simultaneous detection of toxigenic C. difficile in stool and potentially differentiated between RT027 and non-RT027.
关键词: Clostridium difficile multiplex PCR capillary electrophoresis detection characterization
标题 作者 时间 类型 操作
Erratum to: Using pyrosequencing and quantitative PCR to analyze microbial communities
Husen ZHANG
期刊论文
Safety evaluation of microbial pesticide (HaNPV) based on PCR method
Miao Zhao, Shufei Li, Qinghong Zhou, Dianming Zhou, Ning He, Zhiyong Qian
期刊论文
A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host
Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,
期刊论文
Abundance and distribution of ammonia-oxidizing archaea in Tibetan and Yunnan plateau agricultural soils of China
Kun DING,Xianghua WEN,Liang CHEN,Daishi HUANG,Fan FEI,Yuyang LI
期刊论文
Interaction and independence on methane oxidation of landfill cover soil among three impact factors: water, oxygen and ammonium
Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO
期刊论文
Assessment of antibiotic resistance genes in dialysis water treatment processes
Xuan Zhu, Chengsong Ye, Yuxin Wang, Lihua Chen, Lin Feng
期刊论文
Seasonal microbial community shift in a saline sewage treatment plant
Qingmei YAN, Xuxiang ZHANG, Tong ZHANG, Herbert H P FANG
期刊论文
662 A/G gene variation in human tumor necrosis factor receptor superfamily, member 9 (TNFRSF9)
QU Yanchun, YANG Ze, SUN Liang, JI Linong
期刊论文