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《农业科学与工程前沿(英文)》 2021年 第8卷 第2期
Weeping species are used both as ornamental plants and for breeding dwarf plant types. However, exploration of casual genes controlling weeping traits is rather limited. Here, we identified individuals with contrasting phenotypes from an F1 bi-parental mapping population of Prunus mume which was developed from a cross between the upright cultivar ‘Liuban’ and the weeping cultivar ‘Fentai Chuizhi’. Bulked segregant RNA sequencing was used and five QTLs on Chromosome 7 were identified. The Pm024074(PmUGT72B3) allele, belonging to the UDP-glycosyltransferase superfamily containing the coniferyl-alcohol glucosyltransferase domain, was identified in a genomic region overlapping with a previously identified QTL, and had a synonymous transition of T66(upright) to C (weeping) in the coding sequence and a 470-bp deletion in the promoter region. Pm024074 had exceptionally high expression in buds and stems of weeping P. mume. Weighted correlation network analysis indicates that genes neighboring Pm024074 were significantly associated with plant architecture. In addition, a reliable single nucleotide polymorphism marker was developed based on the variation in the Pm024074 gene, providing precise marker-assisted breeding for weeping traits. This study provides insights into the genetic mechanism governing the weeping trait in P. mume, and indicates potential applications for the manipulation of tree architecture.
关键词: BSR-seq / PmUGT72B3 / Prunus mume / UDP-glycosyltransferase / weeping shoots / WGCNA
Xiaokang ZHUO, Tangchun ZHENG, Zhiyong ZHANG, Suzhen LI, Yichi ZHANG, Lidan SUN, Weiru YANG, Jia WANG, Tangren CHENG, Qixiang ZHANG
《农业科学与工程前沿(英文)》 页码 196-214 doi: 10.15302/J-FASE-2020379
关键词: BSR-seq PmUGT72B3 Prunus mume UDP-glycosyltransferase weeping shoots WGCNA
《医学前沿(英文)》 2022年 第16卷 第6期 页码 883-895 doi: 10.1007/s11684-022-0919-0
关键词: osteosarcoma autophagy metastasis drug resistance Beclin1 LC3B
Hong SUN,Min SUN,Yaobin ZHANG,Xie QUAN
《环境科学与工程前沿(英文)》 2015年 第9卷 第4期 页码 591-595 doi: 10.1007/s11783-014-0694-5
关键词: catalytic ozonation reactive red X-3B ceramic honeycomb hydroxyl radical (OH·)
《医学前沿(英文)》 2022年 第16卷 第1期 页码 139-149 doi: 10.1007/s11684-021-0835-8
关键词: B-cell acute lymphoblastic leukemia bispecific antibody trispecific antibody CD19 CD20
LI Yanhua, BI Zhigang
《医学前沿(英文)》 2007年 第1卷 第1期 页码 79-86 doi: 10.1007/s11684-007-0016-4
王振占,张升伟,李靖,李芸,吴琼
《中国工程科学》 2013年 第15卷 第10期 页码 33-46
本文论述了风云三号卫星B星微波湿度计(MWHS-3B)发射前热真空定标的原理和方法,建立了各种定标误差的修正算法,获得了MWHS-3B的系统线性度这些参数已经用于MWHS-3B在轨数据的处理。
李长东,冯颖,陈素文,聂东云,李坚,张为远
《中国工程科学》 2014年 第16卷 第5期 页码 20-23
Development of the expressed immunoglobulin μ chain repertoire during maturation of mice B cells
Jingwen LIANG,Yingfeng LUO,Yi SUN,Meng LEI,Bing ZHANG,Songnian HU,Yaofeng ZHAO
《农业科学与工程前沿(英文)》 2014年 第1卷 第3期 页码 201-213 doi: 10.15302/J-FASE-2014017
关键词: repertoire complementarity determining region 3 of the IgH chain (CDRH3) immunoglobulin heavy chain variable region
Stiffness of a 3-degree of freedom translational parallel kinematic machine
null
《机械工程前沿(英文)》 2014年 第9卷 第3期 页码 233-241 doi: 10.1007/s11465-014-0312-z
In this paper, a typical 3-degree of freedom (3-DOF) translational parallel kinematic machine (PKM) is studied and analyzed whose tool platform has only translations along X-, Y- and Z-axes. It consists of three limbs, each of which have arm and forearm with prismatic-revolute-revolute-revolute (PRRR) joints. Inverse kinematics analysis is carried out to find the slider coordinates and joint angles for a given position of tool platform. Stiffness modeling is done based on the compliance matrices of arm and forearm of each limb. Using the stiffness modeling the variations of minimum and maximum translational stiffness in the workspace are analyzed. For various architectural parameters of the 3-DOF PKM the tendency of variations on the minimum and maximum stiffness over the entire workspace is studied; and also the deflections of the tool platform along X, Y, and Z directions with respect to various forces are presented.
关键词: 3-DOF translational PKM inverse kinematics stiffness modeling translational stiffness
131I标记的人源化抗B7-H3抗体用于胶质母细胞瘤放射免疫疗法的治疗特性 Article
傅丰庆, Meng Zheng, Shandong Zhao, Yan Wang, Minzhou Huang, Hanqing Chen, Ziyi Huang, Kaijie Zhang, 缪丽燕, 张学光
《工程(英文)》 2023年 第30卷 第11期 页码 190-202 doi: 10.1016/j.eng.2023.05.011
B7 homolog 3 (B7-H3) has attracted much attention in glioblastoma (GBM) radioimmunotherapy (RIT) due to its abnormally high expression on tumor cells. In this study, we report that two specific humanized anti-human B7-H3 antibodies (hu4G4 and hu4H12) derived from mouse anti-human B7-H3 antibodies that were generated by computer-aided design and exclusively recognize membrane expression of B7-H3 by human glioma cells. Hu4G4 and hu4H12 were radiolabeled with 89Zr for RIT antibody screening. Micro-positron emission tomography (PET) imaging, biodistribution and pharmacokinetic (PK) analyses of 89Zr-labeled antibodies were performed in U87-xenografted models. 125I labelling of the antibodies for single-photon emission computed tomography (SPECT) imaging was also used to investigate the biological behavior of the antibodies in vivo. Furthermore, the pharmacodynamic (PD) of the 131Ilabeled antibodies were evaluated in U87-xenografted mice and GL261 Red-FLuc-B7-H3 in situ glioma tumor models. Micro-PET imaging and biodistribution analysis with a gamma counter showed that 89Zr-deferoxamine (DFO)-hu4G4 had higher tumor targeting performance with lower liver uptake than 89Zr-DFO (hu4H12, immunoglobulin G (IgG)). The biodistribution results of 125I-SPECT imaging were similar to those of 89Zr-PET imaging, though the biodistribution in long bone joints and the thyroid varied. The PD analysis results indicated that 131I-hu4G4 had an excellent therapeutic effect and high safety with no apparent toxicity. Interestingly, 131I-hu4G4 improved the tumor vasculature in tissues with higher expression of collagen type IV and platelet-derived growth factor receptor b (PDGFR-b) compared with control treatment, as determined by immunofluorescence (IF), which contributed to inhibiting tumor growth. Taken together, our data indicate that hu4G4 exhibits good tumor targeting and specificity, achieves low nonspecific concentrations in normal tissues, and has acceptable PK characteristics. 131I-hu4G4 also exerts effective antitumor effects with an ideal safety profile. Therefore, we expect hu4G4 to be an excellent antibody for the development of GBM RIT.
关键词: B7-H3 Radioimmunotherapy Glioblastoma Pharmacokinetics Pharmacodynamics
组织工程和给药技术中的三维光制造 Review
Bártolo
《工程(英文)》 2015年 第1卷 第1期 页码 90-112 doi: 10.15302/J-ENG-2015015
基于双目立体匹配和B样条的三维人脸点云孔洞修补算法 Research Articles
黄源1,2,达飞鹏1,2
《信息与电子工程前沿(英文)》 2022年 第23卷 第3期 页码 398-408 doi: 10.1631/FITEE.2000508
关键词: 三维点云;孔洞修补;立体匹配;B样条
Error analysis and optimization of a 3-degree of freedom translational Parallel Kinematic Machine
null
《机械工程前沿(英文)》 2014年 第9卷 第2期 页码 120-129 doi: 10.1007/s11465-014-0300-3
In this paper, error modeling and analysis of a typical 3-degree of freedom translational Parallel Kinematic Machine is presented. This mechanism provides translational motion along the Cartesian X-, Y- and Z- axes. It consists of three limbs each having an arm and forearm with prismatic-revolute-revolute-revolute joints. The moving or tool platform maintains same orientation in the entire workspace due to its joint arrangement. From inverse kinematics, the joint angles for a given position of tool platform necessary for the error modeling and analysis are obtained. Error modeling is done based on the differentiation of the inverse kinematic equations. Variation of pose errors along X, Y and Z directions for a set of dimensions of the parallel kinematic machine is presented. A non-dimensional performance index, namely, global error transformation index is used to study the influence of dimensions and its corresponding global maximum pose error is reported. An attempt is made to find the optimal dimensions of the Parallel Kinematic Machine using Genetic Algorithms in MATLAB. The methodology presented and the results obtained are useful for predicting the performance capability of the Parallel Kinematic Machine under study.
关键词: translational Parallel Kinematic Machine error modeling global error transformation index
Multi-objective optimization of a 3D vaneless diffuser based on fuzzy theory
GAO Chuang, GU Chuangang, WANG Tong, SHU Xinwei
《能源前沿(英文)》 2008年 第2卷 第1期 页码 66-70 doi: 10.1007/s11708-008-0006-5
关键词: Computational pressure recovery B-spline technology corresponding Interactive
标题 作者 时间 类型 操作
BULKED SEGREGANT RNA SEQUENCING (BSR-SEQ) IDENTIFIES A NOVEL ALLELE ASSOCIATED WITH WEEPING TRAITS IN PRUNUS MUME
期刊论文
BULKED SEGREGANT RNA SEQUENCING (BSR-SEQ) IDENTIFIES A NOVEL ALLELE ASSOCIATED WITH WEEPING TRAITS IN
Xiaokang ZHUO, Tangchun ZHENG, Zhiyong ZHANG, Suzhen LI, Yichi ZHANG, Lidan SUN, Weiru YANG, Jia WANG, Tangren CHENG, Qixiang ZHANG
期刊论文
Superenhancers activate the autophagy-related genes Beclin1 and LC3B to drive metastasis and drug resistance
期刊论文
Catalytic ozonation of reactive red X-3B in aqueous solution under low pressure: decolorization and OH
Hong SUN,Min SUN,Yaobin ZHANG,Xie QUAN
期刊论文
Preclinical characterization and comparison between CD3/CD19 bispecific and novel CD3/CD19/CD20 trispecificantibodies against B-cell acute lymphoblastic leukemia: targeted immunotherapy for acute lymphoblastic
期刊论文
Ultraviolet-B induced expression of hypoxia-inducible factor 1α, transferrin receptor through EGFR/PI3K
LI Yanhua, BI Zhigang
期刊论文
Development of the expressed immunoglobulin μ chain repertoire during maturation of mice B cells
Jingwen LIANG,Yingfeng LUO,Yi SUN,Meng LEI,Bing ZHANG,Songnian HU,Yaofeng ZHAO
期刊论文
131I标记的人源化抗B7-H3抗体用于胶质母细胞瘤放射免疫疗法的治疗特性
傅丰庆, Meng Zheng, Shandong Zhao, Yan Wang, Minzhou Huang, Hanqing Chen, Ziyi Huang, Kaijie Zhang, 缪丽燕, 张学光
期刊论文
Error analysis and optimization of a 3-degree of freedom translational Parallel Kinematic Machine
null
期刊论文