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《农业科学与工程前沿(英文)》 2021年 第8卷 第2期
Late embryogenesis abundant (LEA) genes encode highly hydrophilic proteins that are essential in abiotic stress responses. However, most LEA genes in higher plants have not yet been investigated. This study identified an LEA family gene (PtrLEA7) from Poncirus trifoliata and studied its function in drought tolerance. The full-length coding sequence of PtrLEA7 was 420 bp encoding a protein of 139 amino acids. Phylogenetic analysis shows that PtrLEA7 protein belongs to the LEA_4 subfamily. Expression profiling by qPCR found that PtrLEA7 was strongly induced by dehydration, cold and ABA treatments, and slightly induced by salt stress. Subcellular localization reveals that PtrLEA7 protein was located in both cytoplasm and nucleus. To investigate its function, transgenic plants of both tobacco and Poncirus trifoliata overexpressing PtrLEA7 were obtained. Stress tolerance assays show that overexpression lines had enhanced dehydration and drought tolerance compared with wild type plants, indicating that PtrLEA7 positively regulates drought tolerance. In addition, transgenic plants had much higher expression levels of three antioxidant enzyme genes (CAT, SOD and POD) and significantly increased catalase enzyme activity, accompanied by reduced reactive oxygen species accumulation in comparison with wild type plants. Collectively, this study demonstrates that PtrLEA7 can confer enhanced drought tolerance partially via enhancing antioxidant capacity.
Polyamines and antioxidant defense system are associated with cold tolerance in centipedegrass
Jingjing CHEN, Junyang FANG, Zhenfei GUO, Shaoyun LU
《农业科学与工程前沿(英文)》 2018年 第5卷 第1期 页码 129-138 doi: 10.15302/J-FASE-2017197
关键词: antioxidants centipedegrass chilling photosynthesis polyamines
Tonglu WEI, Dalong GUO, Jihong LIU
《农业科学与工程前沿(英文)》 页码 236-246 doi: 10.15302/J-FASE-2020368
关键词: abiotic stress antioxidant drought late embryogenesis abundant Poncirus trifoliata
Xiaohong Kou,Lihua Han,Xingyuan Li,Zhaohui Xue,Fengjuan Zhou
《化学科学与工程前沿(英文)》 2016年 第10卷 第1期 页码 108-119 doi: 10.1007/s11705-016-1553-7
关键词: crude polyphenol extract purified polyphenol extract blueberry antioxidant antitumor immunity
Inhibition of NO emission by adding antioxidant mixture in
A. PRABU,R. B. ANAND
《能源前沿(英文)》 2015年 第9卷 第2期 页码 238-245 doi: 10.1007/s11708-015-0356-8
关键词: NO emission antioxidants Succinimide N N-dimethyl-p-phenylenediamine-dihydrochloride N-phenyl-p-phenylenediamine
Zinc homeostasis in the metabolic syndrome and diabetes
null
《医学前沿(英文)》 2013年 第7卷 第1期 页码 31-52 doi: 10.1007/s11684-013-0251-9
Zinc (Zn) is an essential mineral that is required for various cellular functions. Zn dyshomeostasis always is related to certain disorders such as metabolic syndrome, diabetes and diabetic complications. The associations of Zn with metabolic syndrome, diabetes and diabetic complications, thus, stem from the multiple roles of Zn: (1) a constructive component of many important enzymes or proteins, (2) a requirement for insulin storage and secretion, (3) a direct or indirect antioxidant action, and (4) an insulin-like action. However, whether there is a clear cause-and-effect relationship of Zn with metabolic syndrome, diabetes, or diabetic complications remains unclear. In fact, it is known that Zn deficiency is a common phenomenon in diabetic patients. Chronic low intake of Zn was associated with the increased risk of diabetes and diabetes also impairs Zn metabolism. Theoretically Zn supplementation should prevent the metabolic syndrome, diabetes, and diabetic complications; however, limited available data are not always supportive of the above notion. Therefore, this review has tried to summarize these pieces of available information, possible mechanisms by which Zn prevents the metabolic syndrome, diabetes, and diabetic complications. In the final part, what are the current issues for Zn supplementation were also discussed.
关键词: zinc zinc transporters metallothionein diabetes diabetic complications insulin resistance antioxidant
Yuping ZHANG, Yanfei ZHOU, Xingxu ZHANG, Tingyu DUAN, Zhibiao NAN
《农业科学与工程前沿(英文)》 2018年 第5卷 第1期 页码 148-158 doi: 10.15302/J-FASE-2017195
关键词: antioxidant enzymes Elymus dahuricus Epichloë fungal endophyte photosynthesis
Joint effects of Penta-BDE and heavy metals on
Baohua TANG, Lingyan ZHU, Qixing ZHOU
《环境科学与工程前沿(英文)》 2011年 第5卷 第1期 页码 99-110 doi: 10.1007/s11783-010-0260-8
关键词: polybrominated diphenyl ethers (PBDEs) heavy metal mixture toxicity toxic units (TUs) antioxidant enzyme lipid peroxidation
EXTRACTION AND EVALUATION OF EDIBLE OIL FROM SCHIZOCHYTRIUM SP. USING AN AQUEOUS ENZYMATIC METHOD
《农业科学与工程前沿(英文)》 2021年 第8卷 第4期 页码 623-634 doi: 10.15302/J-FASE -2021400
Schizochytrium sp., a marine microalga, is a potential source of edible oil due to its short growth cycle and rapid lipid accumulation, especially of docosahexaenoic acid. An approach to isolate edible microalgal oil from Schizochytrium sp. using aqueous enzymatic extraction (AEE) was developed. Parameters were optimized by single-factor experiments followed by Box-Behnken design. Proteases were effective in extracting oil. The maximum free oil recovery (49.7%±0.58%) and total oil recovery (68.1%±0.94%) were obtained under optimum conditions of liquid-to-solid ratio of 4.8:1, a 2.5% enzyme concentration of papain and an extraction time of 2.2 h. There was a significant difference (P<0.05) in polyunsaturated fatty acid composition between microalgal oil obtained by AEE and by Soxhlet extraction, with the former having superior physiochemical properties and higher concentrations of bioactive components including total phenolic compounds and total tocopherols. These findings indicate a potential application of AEE for extraction of oil from Schizochytrium sp.
Single and joint effects of HHCB and cadmium on zebrafish (
Lei ZHANG, Jing AN, Qixing ZHOU
《环境科学与工程前沿(英文)》 2012年 第6卷 第3期 页码 360-372 doi: 10.1007/s11783-011-0353-z
关键词: 1 3 4 6 7 8-hexahydro-4 6 6 7 8 8-hexamethylcyclopenta-gamma-2-benzopyran (HHCB) cadmium (Cd) antioxidant biomarker feculent water containing bedloads
Antibacterial properties of extracts of
Manhong YE,Lei ZHANG,Jiaqi GE,Haifeng SUN,Jingjing NI,Shengmei YANG,Wanhong WEI
《农业科学与工程前沿(英文)》 2015年 第2卷 第4期 页码 327-334 doi: 10.15302/J-FASE-2015078
关键词: antibacterial activity Canadian goldenrod antioxidant enzyme crayfish
Oxidative stress and diabetes: antioxidative strategies
Pengju Zhang, Tao Li, Xingyun Wu, Edouard C. Nice, Canhua Huang, Yuanyuan Zhang
《医学前沿(英文)》 2020年 第14卷 第5期 页码 583-600 doi: 10.1007/s11684-019-0729-1
关键词: diabetes oxidative stress redox modification antioxidative therapy novel antioxidant delivery
Performance and synergistic effect of phenolic and thio antioxidants in ABS graft copolymers
Gongsheng LI, Xuhong GUO, Wang NA, Diancheng HAO, Mingyao ZHANG, Huixuan ZHANG, Jun XU
《化学科学与工程前沿(英文)》 2011年 第5卷 第1期 页码 26-34 doi: 10.1007/s11705-010-0531-8
《化学科学与工程前沿(英文)》 2023年 第17卷 第7期 页码 976-989 doi: 10.1007/s11705-022-2237-0
关键词: grape seed lignin structure antioxidant NE-4C cells neuroprotection
解淀粉芽孢杆菌发酵红小豆的理化特性和生物活性 Article
吴珊, 卢帅, 刘军, 杨绍青, 闫巧娟, 江正强
《工程(英文)》 2021年 第7卷 第2期 页码 219-225 doi: 10.1016/j.eng.2020.10.010
本研究采用解淀粉芽孢杆菌发酵红小豆(Vigna umbellata),并评价了发酵红小豆的理化特性和生物活性。以纤溶酶活性为指标优化了发酵条件,在最适发酵条件下,纤溶酶活性最高达78.0 FU∙g-1(4890 IU∙g-1,纤维蛋白平板法)。发酵红小豆中多肽含量(2.1~10.9 g∙100 g-1)、总酚含量(116.7~388.5 mg没食子酸∙100 g-1)、总黄酮含量(235.5~354.3 mg芦丁∙100 g-1)、花青素含量(20.1~47.1 mg∙100 g-1)和超氧化物歧化酶活性(55.3~263.6 U∙g-1)显著增加。红小豆经发酵后的2,2-二苯基-1-苦肼基(DPPH)和2,2-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基清除活性和铁离子还原抗氧化能力(FRAP)较未发酵的红小豆提高1.9~4.8倍。此外,发酵红小豆的二肽基肽酶Ⅳ(DPP-Ⅳ)抑制活性、α-葡萄糖苷酶抑制活性和抗凝血活性显著提高。解淀粉芽孢杆菌发酵红小豆具有多种功能活性,对开发预防血栓性疾病的功能食品具有重要意义。
标题 作者 时间 类型 操作
EMBRYOGENESIS ABUNDANT FAMILY GENE FROM PONCIRUS TRIFOLIATA, CONFERS ENHANCED DROUGHT TOLERANCE BY ENHANCING ANTIOXIDANT
期刊论文
Polyamines and antioxidant defense system are associated with cold tolerance in centipedegrass
Jingjing CHEN, Junyang FANG, Zhenfei GUO, Shaoyun LU
期刊论文
Antioxidant and antitumor effects and immunomodulatory activities of crude and purified polyphenol extract
Xiaohong Kou,Lihua Han,Xingyuan Li,Zhaohui Xue,Fengjuan Zhou
期刊论文
EXTRACTION AND EVALUATION OF EDIBLE OIL FROM SCHIZOCHYTRIUM SP. USING AN AQUEOUS ENZYMATIC METHOD
期刊论文
Antibacterial properties of extracts of
Manhong YE,Lei ZHANG,Jiaqi GE,Haifeng SUN,Jingjing NI,Shengmei YANG,Wanhong WEI
期刊论文
Oxidative stress and diabetes: antioxidative strategies
Pengju Zhang, Tao Li, Xingyun Wu, Edouard C. Nice, Canhua Huang, Yuanyuan Zhang
期刊论文
Performance and synergistic effect of phenolic and thio antioxidants in ABS graft copolymers
Gongsheng LI, Xuhong GUO, Wang NA, Diancheng HAO, Mingyao ZHANG, Huixuan ZHANG, Jun XU
期刊论文
Protective effects of lignin fractions obtained from grape seeds against bisphenol AF neurotoxicity via antioxidative effects mediated by the Nrf2 pathway
期刊论文