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乙醇 1

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木质纤维原料 1

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木质纤维原料制乙醇原料预处理技术

靳胜英,张福琴,张礼安

《中国工程科学》 2008年第10卷第11期页码 82-88

摘要：

从预处理的必要性出发，论述了木质纤维原料的预处理技术，分析了主要预处理技术的特点和处理纤维质原料的效果，并对未来预处理技术的发展方向提出了建议，供从事木质纤维素制乙醇的研究人员参考。

关键词：木质纤维原料乙醇预处理技术

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生物燃料乙醇产业未来发展的新模式

王梦,田晓俊,陈必强,林海龙,岳国君

《中国工程科学》 2020年第22卷第2期页码 47-54 doi: 10.15302/J-SSCAE-2020.02.007

摘要：

燃料乙醇产业是我国重点培育和发展的战略性新兴产业之一，在国家推进工业化与信息化深度融合的背景下，利用我国在工业互联网和第五代移动通信（5G）技术上的优势，以大数据、数字孪生和区块链等新技术为支撑，推进生物燃料乙醇产业的智能化、安全化发展新模式，对于我国燃料乙醇产业高质量发展具有重要意义。本文梳理并总结了国内外生物燃料乙醇产业的发展现状，凝练产业智能化、安全化发展面临的问题，提出了生物燃料乙醇产业智能生产新模式、安全生产新模式和产业管理新模式的总体思路。研究表明，我国生物燃料乙醇产业发展应在国家、地方和企业的保障与积极配合下，从国家战略层面引导产业发展，推进生物燃料乙醇产业基地特色化、联合化、智能化发展；加大“产学研”合作力度，创新生产模式和生产技术，提升国际市场竞争力；进一步开展关键技术攻关，实现以纤维素类生物质为原料的生物燃料乙醇技术突破，为国家粮食安全问题提供战略储备。

关键词：燃料乙醇智能生产安全生产新模式

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Advances in the study of directed evolution for cellulases

Hailong LIN, Weiguang LI, Changhong GUO, Sihang QU, Nanqi REN

《环境科学与工程前沿（英文）》 2011年第5卷第4期页码 519-525 doi: 10.1007/s11783-011-0326-2

摘要： If cellulose can be effectively hydrolyzed into glucose by cellulase, the production costs of hydrogen, ethanol or other chemicals from cellulosic materials will be greatly decreased, and economically viable production of biohydrogen and bioethanol will become feasible. Cellulose is degraded into glucoses by multi-component enzyme systems. Nowadays cellulases are widely used in brewing, food, bioenergy, fodder, textiles, paper, pharmaceuticals, environmental protection and other industries. However, existing cellulases have several problems that limit their wider applications, including the low turnover number for solid cellulosic materials, and low stability in adapting to various application conditions. For example, high temperature, low pH, and so on. Application of directed evolution technology may be one of the most effective ways for improving the characteristics of cellulases. This paper presents a brief review of the cellulose hydrolysis mechanism by cellulase, advances in cellulases (endoglucanase and -glucosidase) improvement by directed evolution for several characteristics (for instance, thermal stability, pH adaptability and enzyme activity), limitations of directed evolution for cellulases, and the outlook for directed evolution for cellulase.

关键词： biohydrogen bioethanol cellulase cellulose directed evolution

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Simultaneous saccharification and fermentation of wheat bran flour into ethanol using coculture of amylotic

K. Manikandan, T. Viruthagiri

《化学科学与工程前沿（英文）》 2009年第3卷第3期页码 240-249 doi: 10.1007/s11705-009-0205-6

摘要： Studies on simultaneous saccharification and fermentation (SSF) of wheat bran flour, a grain milling residue as the substrate using coculture method were carried out with strains of starch digesting and nonstarch digesting and sugar fermenting in batch fermentation. Experiments based on central composite design (CCD) were conducted to maximize the glucose yield and to study the effects of substrate concentration, pH, temperature, and enzyme concentration on percentage conversion of wheat bran flour starch to glucose by treatment with fungal α-amylase and the above parameters were optimized using response surface methodology (RSM). The optimum values of substrate concentration, pH, temperature, and enzyme concentration were found to be 200 g/L, 5.5, 65°C and 7.5 IU, respectively, in the starch saccharification step. The effects of pH, temperature and substrate concentration on ethanol concentration, biomass and reducing sugar concentration were also investigated. The optimum temperature and pH were found to be 30°C and 5.5, respectively. The wheat bran flour solution equivalent to 6% ( / ) initial starch concentration gave the highest ethanol concentration of 23.1 g/L after 48 h of fermentation at optimum conditions of pH and temperature. The growth kinetics was modeled using Monod model and Logistic model and product formation kinetics using Leudeking-Piret model. Simultaneous saccharificiation and fermentation of liquefied wheat bran starch to bioethanol was studied using coculture of amylolytic fungus and nonamylolytic sugar fermenting .

关键词： simultaneous saccharification and fermentation (SSF) starch coculture fermentation statistical experimental design bioethanol Monod model

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Simultaneous saccharification and fermentation of sweet potato powder for the production of ethanol under conditions of very high gravity

Yinxiu CAO, Hongchi TIAN, Kun YAO, Yingjin YUAN

《化学科学与工程前沿（英文）》 2011年第5卷第3期页码 318-324 doi: 10.1007/s11705-010-1026-3

摘要： Due to its merits of drought tolerance and high yield, sweet potatoes are widely considered as a potential alterative feedstock for bioethanol production. Very high gravity (VHG) technology is an effective strategy for improving the efficiency of ethanol fermentation from starch materials. However, this technology has rarely been applied to sweet potatoes because of the high viscosity of their liquid mash. To overcome this problem, cellulase was added to reduce the high viscosity, and the optimal dosage and treatment time were 8 U/g (sweet potato powder) and 1 h, respectively. After pretreatment by cellulase, the viscosity of the VHG sweet potato mash (containing 284.2 g/L of carbohydrates) was reduced by 81%. After liquefaction and simultaneous saccharification and fermentation (SSF), the final ethanol concentration reached 15.5% (v/v), and the total sugar conversion and ethanol yields were 96.5% and 87.8%, respectively.

关键词： bioethanol sweet potato very high gravity viscosity reduction simultaneous saccharification and fermentation