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期刊论文 3

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hsa-miR-197 1

子宫肌瘤 1

生物信息学 1

靶基因 1

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hsa-miR-197在子宫肌瘤中的表达及生物信息学特征分析

徐青,付子毅,吴小莉,皇甫玉爽,凌静

《中国工程科学》 2014年 第16卷 第5期   页码 99-104

摘要:

应用实时定量聚合酶链式反应(PCR)技术检测子宫肌瘤组织中的hsa-miR-197 表达水平,并与配对正常子宫肌细胞对比。通过miRbase、UCSC、NCBI等在线数据库获取并分析hsa-miR-197 序列保守性及其基因组特征。选择miRanda、MirTarget2 及TargetScan 三个在线数据库预测hsa-miR-197 的靶基因并取交集以便后续分析。通过基因本体(GO)功能注释、GO富集分析和信号转导通路富集分析初步阐明,hsamiR-197 靶基因参与调控的细胞功能与信号通路。hsa-miR-197 的功能较广泛,参与肿瘤发生的多种生物学过程,提示hsa-miR-197 可能与子宫肌瘤的发病机制密切相关。

关键词: 子宫肌瘤     hsa-miR-197     生物信息学     靶基因    

Bioinformatic exploration of MTA1-regulated gene networks in colon cancer

null

《医学前沿(英文)》 2016年 第10卷 第2期   页码 178-182 doi: 10.1007/s11684-016-0442-2

摘要:

Metastasis-associated gene 1 (MTA1) controls a series of biological processes in tumor progression. Tumor progression is a complex process regulated by a gene network. The global cancer gene regulatory network must be analyzed to determine the position of MTA1 in the molecular network and its cooperative genes by further exploring the biological functions of this gene. We used TCGA data sets and GeneCards database to screen MTA1-related genes. GO and KEGG pathway analyses were conducted with DAVID and gene network analysis via STRING and Cytoscape. Results showed that in the development of colon cancer, MTA1 is linked to certain signal pathways, such as Wnt/Notch/nucleotide excision repair pathways. The findings also suggested that MTA1 demonstrates the closest relationship in a coregulation process with the key molecules AKT1, EP300, CREBBP, SMARCA4, RHOA, and CAD. These results lead MTA1 exploration to an in-depth investigation in different directions, such as Wnt, Notch, and DNA repair.

关键词: metastasis-associated gene 1     colon cancer     bioinformatics    

iTRAQ-based quantitative proteomic analysis on differentially expressed proteins of rat mandibular condylar cartilage induced by reducing dietary loading

null

《医学前沿(英文)》 2017年 第11卷 第1期   页码 97-109 doi: 10.1007/s11684-016-0496-1

摘要:

As muscle activity during growth is considerably important for mandible quality and morphology, reducing dietary loading directly influences the development and metabolic activity of mandibular condylar cartilage (MCC). However, an overall investigation of changes in the protein composition of MCC has not been fully described in literature. To study the protein expression and putative signaling in vivo, we evaluated the structural changes of MCC and differentially expressed proteins induced by reducing functional loading in rat MCC at developmental stages. Isobaric tag for relative and absolute quantitation-based 2D nano-high performance liquid chromatography (HPLC) and matrix-assisted laser desorption/ionization time-of-flight/ time-of-flight (MALDI-TOF/TOF) technologies were used. Global protein profiling, KEGG and PANTHER pathways, and functional categories were analyzed. Consequently, histological and tartrate-resistant acid phosphatase staining indicated the altered histological structure of condylar cartilage and increased bone remodeling activity in hard-diet group. A total of 805 differentially expressed proteins were then identified. GO analysis revealed a significant number of proteins involved in the metabolic process, cellular process, biological regulation, localization, developmental process, and response to stimulus. KEGG pathway analysis also suggested that these proteins participated in various signaling pathways, including calcium signaling pathway, gap junction, ErbB signaling pathway, and mitogen-activated protein kinase signaling pathway. Collagen types I and II were further validated by immunohistochemical staining and Western blot analysis. Taken together, the present study provides an insight into the molecular mechanism of regulating condylar growth and remodeling induced by reducing dietary loading at the protein level.

关键词: condylar cartilage     mechanical loading     proteomic analysis     iTRAQ     bioinformatics analysis    

标题 作者 时间 类型 操作

hsa-miR-197在子宫肌瘤中的表达及生物信息学特征分析

徐青,付子毅,吴小莉,皇甫玉爽,凌静

期刊论文

Bioinformatic exploration of MTA1-regulated gene networks in colon cancer

null

期刊论文

iTRAQ-based quantitative proteomic analysis on differentially expressed proteins of rat mandibular condylar cartilage induced by reducing dietary loading

null

期刊论文