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Real time monitoring of bioreactor mAb IgG3 cell culture process dynamics via Fourier transform infraredspectroscopy: Implications for enabling cell culture process analytical technology?

Huiquan Wu, Erik Read, Maury White, Brittany Chavez, Kurt Brorson, Cyrus Agarabi, Mansoor Khan

《化学科学与工程前沿(英文)》 2015年 第9卷 第3期   页码 386-406 doi: 10.1007/s11705-015-1533-3

摘要: Compared to small molecule process analytical technology (PAT) applications, biotechnology product PAT applications have certain unique challenges and opportunities. Understanding process dynamics of bioreactor cell culture process is essential to establish an appropriate process control strategy for biotechnology product PAT applications. Inline spectroscopic techniques for real time monitoring of bioreactor cell culture process have the distinct potential to develop PAT approaches in manufacturing biotechnology drug products. However, the use of inline Fourier transform infrared (FTIR) spectroscopic techniques for bioreactor cell culture process monitoring has not been reported. In this work, real time inline FTIR Spectroscopy was applied to a lab scale bioreactor mAb IgG3 cell culture fluid biomolecular dynamic model. The technical feasibility of using FTIR Spectroscopy for real time tracking and monitoring four key cell culture metabolites (including glucose, glutamine, lactate, and ammonia) and protein yield at increasing levels of complexity (simple binary system, fully formulated media, actual bioreactor cell culture process) was evaluated via a stepwise approach. The FTIR fingerprints of the key metabolites were identified. The multivariate partial least squares (PLS) calibration models were established to correlate the process FTIR spectra with the concentrations of key metabolites and protein yield of in-process samples, either individually for each metabolite and protein or globally for all four metabolites simultaneously. Applying the 2 derivative pre-processing algorithm to the FTIR spectra helps to reduce the number of PLS latent variables needed significantly and thus simplify the interpretation of the PLS models. The validated PLS models show promise in predicting the concentration profiles of glucose, glutamine, lactate, and ammonia and protein yield over the course of the bioreactor cell culture process. Therefore, this work demonstrated the technical feasibility of real time monitoring of the bioreactor cell culture process via FTIR spectroscopy. Its implications for enabling cell culture PAT were discussed.

关键词: process analytical technology (PAT)     Fourier-transform infrared (FTIR) spectroscopy     partial least squares (PLS) regression     mouse IgG3     bioreactor cell culture process     real time process monitoring    

Techno-economic characteristics of wastewater treatment plants retrofitted from the conventional activated sludge processto the membrane bioreactor process

《环境科学与工程前沿(英文)》 doi: 10.1007/s11783-021-1483-6

摘要:

While a growing number of wastewater treatment plants (WWTPs) are being retrofitted from the conventional activated sludge (CAS) process to the membrane bioreactor (MBR) process, the debate on the techno-economy of MBR vs. CAS has continued and calls for a thorough assessment based on techno-economic valuation.

关键词: Membrane bioreactor (MBR)     Conventional activated sludge (CAS)     Cost-benefit analysis     Data envelopment analysis     Net profit    

Positive stool culture could predict the clinical outcomes of haploidentical hematopoietic stem cell

Lijuan Hu, Qi Wang, Xiaohui Zhang, Lanping Xu, Yu Wang, Chenhua Yan, Huan Chen, Yuhong Chen, Kaiyan Liu, Hui Wang, Xiaojun Huang, Xiaodong Mo

《医学前沿(英文)》 2019年 第13卷 第4期   页码 492-503 doi: 10.1007/s11684-019-0681-0

摘要: We aimed to identify the effect of positive stool cultures (PSCs) on the clinical outcomes of patients undergoing haploidentical hematopoietic stem cell transplantation (haplo-HSCT) ( = 332). PSCs were observed in 61 patients (PSC group, 18.4%). Enterobacteriaceae in stool specimens was associated with a higher risk of bloodstream infection, and in stool specimens was related to a higher risk of platelet engraftment failure. The cumulative incidence of infection-related mortality 1 year after haplo-HSCT in the PSC group was higher than that of the patients who showed persistently negative stool cultures (NSC group; 19.2% vs. 8.9%, = 0.017). The probabilities of overall survival (71.4% vs. 83.8%, = 0.031) and disease-free survival (69.6% vs. 81.0%, = 0.048) 1 year after haplo-HSCT for the PSC group were significantly lower than those for the NSC group, particularly for patients who had in their stool specimens. In multivariate analysis, in stool specimens significantly increased the risk of mortality and was associated with poorer survival. Our results showed that PSC influenced the clinical outcomes after haplo-HSCT, particularly those who had in their stool specimens.

关键词: haploidentical     hematopoietic stem cell transplantation     stool culture     Candida    

Microfluidics for cell-cell interactions: A review

Rui Li,Xuefei Lv,Xingjian Zhang,Omer Saeed,Yulin Deng

《化学科学与工程前沿(英文)》 2016年 第10卷 第1期   页码 90-98 doi: 10.1007/s11705-015-1550-2

摘要: Microfluidic chip has been applied in various biological fields owing to its low-consumption of reagents, high throughput, fluidic controllability and integrity. The well-designed microscale intermediary is also ideal for the study of cell biology. Particularly, microfluidic chip is helpful for better understanding cell-cell interactions. A general survey of recent publications would help to generalize the designs of the co-culture chips with different features. With ingenious and combinational utilization, the chips facilitate the implementation of some special co-culture models that are highly concerned in a different spatial and temporal way.

关键词: microfluidic chip     co-culture     cell-cell interactions     review    

Performance of bioferric-submerged membrane bioreactor for dyeing wastewater treatment

ZOU Haiyan, XI Danli

《环境科学与工程前沿(英文)》 2007年 第1卷 第3期   页码 374-380 doi: 10.1007/s11783-007-0064-7

摘要: Adding iron salt or iron hydroxide to sludgemixed liquor in an aeration tank of a conventional activated sludge processes (bioferric process) can simultaneously improve the sludge s filterability and enhance the system s treatment capacity. In view of this, Fe(OH) was added to a submerged membrane bioreactor (SMBR) to enhance the removal efficiency and to mitigate membrane fouling. Bioferric process and SMBR were combined to create a novel process called Bioferric-SMBR. A side-by-side comparison study of Bioferric-SMBR and common SMBR dealing with dyeing wastewater was carried out. Bioferric-SMBR showed potential superiority, which could enhance removal efficiency, reduce membrane fouling and improve sludge characteristic. When volumetric loading rate was 25% higher than that of common SMBR, the removal efficiencies of Bioferric-SMBR on COD, dye, and NH-N were 1.0%, 9.5%, and 5.2% higher than that of common SMBR, respectively. The trans-membrane pressure of Bioferric-SMBR was only 36% of that in common SMBR while its membrane flux was 25% higher than that of common SMBR. The stable running period in Bioferric-SMBR was 2.5 times of that in common SMBR when there was no surplus sludge discharged. The mixed liquor suspended solids concentration of Bioferric-SMBR was higher than that of common SMBR with more diversified kinds of microorganisms such as protozoans and metazoans. The mean particle diameter and specific oxygen uptake rate of Bioferric-SMBR were 3.10 and 1.23 times the common SMBR, respectively.

关键词: hydroxide     surplus     aeration     SMBR     Bioferric process    

Dynamic changes of protein corona compositions on the surface of zinc oxide nanoparticle in cell culture

Vo-Van Giau, Yoon-Hee Park, Kyu-Hwan Shim, Sang-Wook Son, Seong-Soo A. An

《化学科学与工程前沿(英文)》 2019年 第13卷 第1期   页码 90-97 doi: 10.1007/s11705-018-1766-z

摘要:

The potential applications of nanomaterials used in nanomedicine as ingredients in drug delivery systems and in other products continue to expand. When nanomaterials are introduced into physiological environments and driven by energetics, they readily associate proteins forming a protein corona (PC) on their surface. This PC could result in an alteration of the nanomaterial’s surface characteristics, affecting their interaction with cells due to conformational changes in adsorbed protein molecules. However, our current understanding of nanobiological interactions is still very limited. Utilizing a liquid chromatography–mass spectroscopy/mass spectroscopy technology and a Cytoscape plugin (ClueGO) approach, we examined the composition of the PC for a set of zinc oxide nanoparticles (ZnONP) from cell culture media typically and further analyzed the biological interaction of identified proteins, respectively. In total, 36 and 33 common proteins were investigated as being bound to ZnONP at 5 min and 60 min, respectively. These proteins were further analyzed with ClueGO, a Cytoscape plugin, which provided gene ontology and the biological interaction processes of identified proteins. Proteins bound to the surface of nanoparticles that may modify the structure, therefore the function of the adsorbed protein could be consequently affect the complicated biological processes.

关键词: ZnONPs     nanoparticles     protein corona     ClueGO    

基于液滴微流控的分级结构反蛋白石多孔支架用于仿生三维细胞共培养 Article

邵长敏, 刘羽霄, 池俊杰, 叶方富, 赵远锦

《工程(英文)》 2021年 第7卷 第12期   页码 1778-1785 doi: 10.1016/j.eng.2020.06.031

摘要:

三维(3D)细胞培养具有更好地模拟天然组织特异性的优势,在药物开发、毒性测试和组织工程中发挥着重要作用。然而,现有的3D细胞培养的支架或微载体通常尺寸有限,并且在模拟生物体内血管复合体方面表现不佳。因此,本研究提出了一种通过简单的微流控方法制备的新型分级结构反蛋白石多孔支架,用于促进3D细胞共培养。该支架是基于微流控乳液液滴模板和惰性聚合物聚合的复合概念构建的。研究结果表明,该支架能够保证细胞培养过程中的营养供给,从而实现大面积的细胞培养。此外,通过在该支架中连续种植不同的细胞,本文还开发了内皮细胞包裹肝细胞的3D细胞共培养系统,用于构建功能化组织。研究结果表明,该支架用于细胞共培养系统,有助于维持肝细胞特定的体内功能。该分级结构反蛋白石多孔支架为3D细胞培养甚至仿生组织的构建奠定了基础。

关键词: 微流控     反蛋白石     细胞培养     液滴     生物材料    

Efficient purification of cell culture-derived classical swine fever virus by ultrafiltration and size-exclusion

Ruining WANG,Yubao ZHI,Junqing GUO,Qingmei LI,Li WANG,Jifei YANG,Qianyue JIN,Yinbiao WANG,Yanyan YANG,Guangxu XING,Songlin QIAO,Mengmeng ZHAO,Ruiguang DENG,Gaiping ZHANG

《农业科学与工程前沿(英文)》 2015年 第2卷 第3期   页码 230-236 doi: 10.15302/J-FASE-2015071

摘要: Large-scale production of cell culture-based classical swine fever virus (CSFV) vaccine is hampered by the adverse reactions caused by contaminants from host cell and culture medium. Hence, we have developed an efficient method for purifying CSFV from cell-culture medium. Pure viral particles were obtained with two steps of tangential-flow filtration (TFF) and size-exclusion chromatography (SEC), and were compared with particles from ultracentrifugation by transmission electron microscopy (TEM), infectivity and recovery test, and real time fluorescent quantitative PCR (FQ-PCR). TFF concentrated the virus particles effectively with a retention rate of 98.5%, and 86.2% of viral particles were obtained from the ultrafiltration retentate through a Sepharose 4 F F column on a biological liquid chromatography system. CSFV purified by TFF-SEC or ultracentrifugation were both biologically active from 1.0×10 TCID ·mL to 3.0×10 TCID ·mL , but the combination of TFF and SEC produced more pure virus particles than by ultracentrifugation alone. In addition, pure CSFV particles with the expected diameter of 40–60 nm were roughly spherical without any visible contamination. Mice immunized with CSFV purified by TFF-SEC produced higher antibody levels compared with immunization with ultracentrifugation-purified CSFV ( <0.05). The purification procedures in this study are reliable technically and feasible for purification of large volumes of viruses.

关键词: classical swine fever virus     virus purification     tangential-flow filtration     size-exclusion chromatography    

Portable fluorescence instrument for detecting membrane integrity in membrane bioreactor (MBR)

《环境科学与工程前沿(英文)》 2024年 第18卷 第2期 doi: 10.1007/s11783-024-1783-8

摘要:

● Double fluorescence peaks model is used to characterize membrane integrity.

关键词: Membrane integrity     Fluorescence spectrum     Portable instrument     Membrane bioreactor    

Reutilize tire in microbial fuel cell for enhancing the nitrogen removal of the anammox process coupled

《环境科学与工程前沿(英文)》 2021年 第15卷 第6期 doi: 10.1007/s11783-021-1409-3

摘要:

• MFC promoted the nitrogen removal of anammox with Fe-C micro-electrolysis.

关键词: Waste tire     MFCs     Micro-electrolysis     Anammox     Feammox    

Methanation and chemolitrophic nitrogen removal by an anaerobic membrane bioreactor coupled partial nitrification

《环境科学与工程前沿(英文)》 2023年 第17卷 第6期 doi: 10.1007/s11783-023-1668-2

摘要:

● Efficient carbon methanation and nitrogen removal was achieved in AnMBR-PN/A system.

关键词: Anaerobic membrane bioreactor     Partial nitrification/Anammox     Carbon separation     Chemolitrophic nitrogen removal    

科技自主创新与中西文化互补之我见——六个典型实例的思考

李世煇

《中国工程科学》 2005年 第7卷 第4期   页码 11-21

摘要:

从中西文化互补的角度,考察了20世纪70年代以来中国科技原始性自主创新的六个典型实例,简述了笔者从事典型信息法研究的几点认识,试图以事实论证:中国传统文化对当代科技自主创新有重大作用,并对其表现形式等做了初步考察。

关键词: 中国传统文化     西方文化     互补     科技自主创新     典型实例    

发酵过程多水平问题及其生物反应器装置技术研究——基于过程参数相关的发酵过程优化与放大技术

张嗣良

《中国工程科学》 2001年 第3卷 第8期   页码 37-45

摘要:

回顾了发酵过程优化与放大所依据的基本思想和方法,认为采用以动力学为基础的最佳工艺控制点为依据的静态操作方法,实质上只是化学工程宏观动力学概念在发酵工程上的延伸,往往忽视细胞代谢流的存在。以细胞代谢流的分析与控制为核心的生物反应工程学的观点,通过实验研究,提出了基于参数相关的发酵过程多水平问题研究的优化技术和发酵过程多参数调整的放大技术。随着过程传感技术和计算机技术的发展,设计了一种定型为FUS-50L(A)的新概念生物反应器。这种新型生物反应器是以物料流检测为手段,过程优化与放大为目标,成功地应用在青霉素、红霉素、金霉素、肌苷、鸟苷发酵和Pichia酵母表达系统的基因工程人血清白蛋白(rh-SA)、疟疾疫苗等高密度高表达培养,大幅度提高发酵水平,并直接放大到几百升,甚至100 m3以上生产规模发酵罐。

关键词: 生物反应器     发酵     优化     放大    

细胞培养肉产业技术发展态势及建议

关欣,周景文,堵国成,陈坚

《中国工程科学》 2023年 第25卷 第3期   页码 251-262 doi: 10.15302/J-SSCAE-2023.07.018

摘要:

细胞培养肉是融合先进生物技术、食品技术的战略性新兴产业,未来有可能带动农业领域的产业结构调整和发展模式转变;细胞培养肉技术正处于向商品化迈进的阶段,开展相关产业技术发展态势分析具有价值。本文梳理了细胞培养肉生产核心原料和试剂的产业态势及研究进展,涵盖良种动物、种子细胞、培养基、支架材料;分析了支持细胞培养肉生产的基础装备设施(生物安全柜、生物反应器及其他常用装备)制造技术瓶颈与行业竞争格局;剖析了细胞培养肉规模化生产的核心技术体系及发展动态,涉及种子细胞库建立、低成本培养基研制、大规模培养工艺、食品化加工技术。针对我国细胞培养肉产业基础的薄弱之处,提出了针对性发展建议:加快试剂装备国产化进程、完善细胞培养肉产业链,完善培养肉技术体系、加强知识产权国际布局,优化学科专业布局、校企融合双元育人,完善产品风险评估、论证和发布团体标准,加强科学知识普及、提高公众接受度。

关键词: 细胞培养肉;种子细胞;生物反应器;大规模培养工艺;培养基研制;食品化加工    

Key stakeholders’ perspectives on the ideal partnering culture in construction projects

Gunnar J. LÜHR, Marian G. C. BOSCH-REKVELDT, Mladen RADUJKOVIC

《工程管理前沿(英文)》 2022年 第9卷 第2期   页码 312-325 doi: 10.1007/s42524-020-0135-z

摘要: This paper examines the current state of project cultures in the German turnkey construction industry and the ideal project cultures in terms of partnering from the perspective of various key stakeholders (i.e., Investors, General Contractors, (Sub-)Contractors and Designers). To investigate the current and ideal cultures, data were gathered among the key stakeholders by means of a survey study with 72 respondents divided over 12 companies. The respondents rated the current and desired cultures by using the Organizational Culture Assessment Instrument, which belongs to the Competing Values Framework. The investigations show many similarities and differences between the stakeholder perspectives of the current and the idealized partnering project cultures. Mainly, the General Contractors desire more cooperative behaviors than the (Sub-)Contractors, and the Investors desire more pronounced flexibility than the General Contractors. All stakeholders desire a cultural change from highly competitive behaviors toward more cooperation. Changes in terms of clear procedures or more flexibility are only desired by the Designers. Defining both the current and an ideal partnering project culture enables academics and project managers to compare their actual project cultures to an ideal situation. With such an approach, academics and project managers could measure whether new tools or changes in resources affect their project cultures toward a partnering project culture.

关键词: project culture     organisational culture     partnering     construction culture     stakeholder perspectives     German    

标题 作者 时间 类型 操作

Real time monitoring of bioreactor mAb IgG3 cell culture process dynamics via Fourier transform infraredspectroscopy: Implications for enabling cell culture process analytical technology?

Huiquan Wu, Erik Read, Maury White, Brittany Chavez, Kurt Brorson, Cyrus Agarabi, Mansoor Khan

期刊论文

Techno-economic characteristics of wastewater treatment plants retrofitted from the conventional activated sludge processto the membrane bioreactor process

期刊论文

Positive stool culture could predict the clinical outcomes of haploidentical hematopoietic stem cell

Lijuan Hu, Qi Wang, Xiaohui Zhang, Lanping Xu, Yu Wang, Chenhua Yan, Huan Chen, Yuhong Chen, Kaiyan Liu, Hui Wang, Xiaojun Huang, Xiaodong Mo

期刊论文

Microfluidics for cell-cell interactions: A review

Rui Li,Xuefei Lv,Xingjian Zhang,Omer Saeed,Yulin Deng

期刊论文

Performance of bioferric-submerged membrane bioreactor for dyeing wastewater treatment

ZOU Haiyan, XI Danli

期刊论文

Dynamic changes of protein corona compositions on the surface of zinc oxide nanoparticle in cell culture

Vo-Van Giau, Yoon-Hee Park, Kyu-Hwan Shim, Sang-Wook Son, Seong-Soo A. An

期刊论文

基于液滴微流控的分级结构反蛋白石多孔支架用于仿生三维细胞共培养

邵长敏, 刘羽霄, 池俊杰, 叶方富, 赵远锦

期刊论文

Efficient purification of cell culture-derived classical swine fever virus by ultrafiltration and size-exclusion

Ruining WANG,Yubao ZHI,Junqing GUO,Qingmei LI,Li WANG,Jifei YANG,Qianyue JIN,Yinbiao WANG,Yanyan YANG,Guangxu XING,Songlin QIAO,Mengmeng ZHAO,Ruiguang DENG,Gaiping ZHANG

期刊论文

Portable fluorescence instrument for detecting membrane integrity in membrane bioreactor (MBR)

期刊论文

Reutilize tire in microbial fuel cell for enhancing the nitrogen removal of the anammox process coupled

期刊论文

Methanation and chemolitrophic nitrogen removal by an anaerobic membrane bioreactor coupled partial nitrification

期刊论文

科技自主创新与中西文化互补之我见——六个典型实例的思考

李世煇

期刊论文

发酵过程多水平问题及其生物反应器装置技术研究——基于过程参数相关的发酵过程优化与放大技术

张嗣良

期刊论文

细胞培养肉产业技术发展态势及建议

关欣,周景文,堵国成,陈坚

期刊论文

Key stakeholders’ perspectives on the ideal partnering culture in construction projects

Gunnar J. LÜHR, Marian G. C. BOSCH-REKVELDT, Mladen RADUJKOVIC

期刊论文