Search | Engineering

订阅投稿

首页学术期刊学术焦点学术视频工程成就工程前沿联系我们 English

资源类型

期刊论文 42

年份

2023 1

2022 5

2021 3

2020 2

2019 5

2017 2

2016 2

2015 3

2013 5

2012 2

2011 2

2009 4

2008 1

2007 3

2004 2

展开︾

关键词

3D打印 2

自体移植 2

骨再生 2

骨髓干细胞 2

免疫微环境调控 1

功能仿生 1

吸收 1

复杂严重髋臼骨缺损 1

大鼠 1

异种复合 1

归元方 1

快速成型 1

掺镁羟基磷灰石 1

新生儿骨质 1

明胶来源 1

有效信号 1

松质骨评价 1

流式细胞分析 1

生物制造 1

展开︾

检索范围：

排序：展示方式：

Current advances for bone regeneration based on tissue engineering strategies

Rui Shi, Yuelong Huang, Chi Ma, Chengai Wu, Wei Tian

《医学前沿（英文）》 2019年第13卷第2期页码 160-188 doi: 10.1007/s11684-018-0629-9

摘要： Bone tissue engineering (BTE) is a rapidly developing strategy for repairing critical-sized bone defects to address the unmet need for bone augmentation and skeletal repair. Effective therapies for bone regeneration primarily require the coordinated combination of innovative scaffolds, seed cells, and biological factors. However, current techniques in bone tissue engineering have not yet reached valid translation into clinical applications because of several limitations, such as weaker osteogenic differentiation, inadequate vascularization of scaffolds, and inefficient growth factor delivery. Therefore, further standardized protocols and innovative measures are required to overcome these shortcomings and facilitate the clinical application of these techniques to enhance bone regeneration. Given the deficiency of comprehensive studies in the development in BTE, our review systematically introduces the new types of biomimetic and bifunctional scaffolds. We describe the cell sources, biology of seed cells, growth factors, vascular development, and the interactions of relevant molecules. Furthermore, we discuss the challenges and perspectives that may propel the direction of future clinical delivery in bone regeneration.

关键词： bone tissue engineering stem cell bone scaffold growth factor bone regeneration

HTML PDF 收藏

FGF13 suppresses acute myeloid leukemia by regulating bone marrow niches

《医学前沿（英文）》 2022年第16卷第6期页码 896-908 doi: 10.1007/s11684-022-0944-z

摘要： Fibroblast growth factor 13 (FGF13) is aberrantly expressed in multiple cancer types, suggesting its essential role in tumorigenesis. Hence, we aimed to explore its definite role in the development of acute myeloid leukemia (AML) and emphasize its associations with bone marrow niches. Results showed that FGF13 was lowly expressed in patients with AML and that its elevated expression was related to prolonged overall survival (OS). Univariate and multivariate Cox regression analyses identified FGF13 as an independent prognostic factor. A prognostic nomogram integrating FGF13 and clinicopathologic variables was constructed to predict 1-, 3-, and 5-year OS. Gene mutation and functional analyses indicated that FGF13 was not associated with AML driver mutations but was related to bone marrow niches. As for immunity, FGF13 was remarkably associated with T cell count, immune checkpoint genes, and cytokines. In addition, FGF13 overexpression substantially inhibited the growth and significantly induced the early apoptosis of AML cells. The xenograft study indicated that FGF13 overexpression prolonged the survival of recipient mice. Overall, FGF13 could serve as an independent prognostic factor for AML, and it was closely related to the bone marrow microenvironment.

关键词： acute myeloid leukemia FGF13 prognosis immune-related genes bone marrow niches

HTML PDF 收藏

Autologous bone marrow stem cell transplantation for the treatment of ulcerative colitis complicated

null

《医学前沿（英文）》 2016年第10卷第4期页码 522-526 doi: 10.1007/s11684-016-0485-4

摘要：

Ulcerative colitis (UC) is a chronic inflammatory bowel disease with continuous or recurrent symptoms. A 42-year-old male patient with intermittent diarrhea accompanied by bloody mucopurulent stools was admitted to our hospital. The diagnosis of UC was confirmed by a combination of laboratory examination, colonoscopy, and histological assay. The patient developed herpes zoster in the hospital, which challenged traditional treatments. Therefore, we performed an autologous bone marrow cells to modulate the immune system with his permission. Autologous bone marrow mononuclear cells were collected and injected locally into the bowel mucosa, and subsequently injected systemically through a peripheral vein. After the patient underwent auto bone marrow mononuclear cells transplantations twice, the patient’s symptoms were alleviated. Furthermore, he recovered from hematochezia, and his hypersensitive C reactive protein decreased. Colonoscopy results showed reduced lesions and decreased areas with bleeding and edema in the sigmoid colon and rectum. No recurrence occurred in the subsequent two years, but long-time monitoring is still necessary for the prophylaxis of colorectal cancer.

关键词： autograft bone marrow stem cells ulcerative colitis cell therapy

HTML PDF 收藏

Machinability of ultrasonic vibration-assisted micro-grinding in biological bone using nanolubricant

《机械工程前沿（英文）》 2023年第18卷第1期 doi: 10.1007/s11465-022-0717-z

摘要： Bone grinding is an essential and vital procedure in most surgical operations. Currently, the insufficient cooling capacity of dry grinding, poor visibility of drip irrigation surgery area, and large grinding force leading to high grinding temperature are the technical bottlenecks of micro-grinding. A new micro-grinding process called ultrasonic vibration-assisted nanoparticle jet mist cooling (U-NJMC) is innovatively proposed to solve the technical problem. It combines the advantages of ultrasonic vibration (UV) and nanoparticle jet mist cooling (NJMC). Notwithstanding, the combined effect of multi parameter collaborative of U-NJMC on cooling has not been investigated. The grinding force, friction coefficient, specific grinding energy, and grinding temperature under dry, drip irrigation, UV, minimum quantity lubrication (MQL), NJMC, and U-NJMC micro-grinding were compared and analyzed. Results showed that the minimum normal grinding force and tangential grinding force of U-NJMC micro-grinding were 1.39 and 0.32 N, which were 75.1% and 82.9% less than those in dry grinding, respectively. The minimum friction coefficient and specific grinding energy were achieved using U-NJMC. Compared with dry, drip, UV, MQL, and NJMC grinding, the friction coefficient of U-NJMC was decreased by 31.3%, 17.0%, 19.0%, 9.8%, and 12.5%, respectively, and the specific grinding energy was decreased by 83.0%, 72.7%, 77.8%, 52.3%, and 64.7%, respectively. Compared with UV or NJMC alone, the grinding temperature of U-NJMC was decreased by 33.5% and 10.0%, respectively. These results showed that U-NJMC provides a novel approach for clinical surgical micro-grinding of biological bone.

关键词： micro-grinding biological bone ultrasonic vibration (UV) nanoparticle jet mist cooling (NJMC) grinding force grinding temperature

HTML PDF 收藏

Hybrid polymer biomaterials for bone tissue regeneration

Bo Lei, Baolin Guo, Kunal J. Rambhia, Peter X. Ma

《医学前沿（英文）》 2019年第13卷第2期页码 189-201 doi: 10.1007/s11684-018-0664-6

摘要： Native tissues possess unparalleled physiochemical and biological functions, which can be attributed to their hybrid polymer composition and intrinsic bioactivity. However, there are also various concerns or limitations over the use of natural materials derived from animals or cadavers, including the potential immunogenicity, pathogen transmission, batch to batch consistence and mismatch in properties for various applications. Therefore, there is an increasing interest in developing degradable hybrid polymer biomaterials with controlled properties for highly efficient biomedical applications. There have been efforts to mimic the extracellular protein structure such as nanofibrous and composite scaffolds, to functionalize scaffold surface for improved cellular interaction, to incorporate controlled biomolecule release capacity to impart biological signaling, and to vary physical properties of scaffolds to regulate cellular behavior. In this review, we highlight the design and synthesis of degradable hybrid polymer biomaterials and focus on recent developments in osteoconductive, elastomeric, photoluminescent and electroactive hybrid polymers. The review further exemplifies their applications for bone tissue regeneration.

关键词： hybrid polymer bone regeneration tissue engineering biomaterials

HTML PDF 收藏

Progress of three-dimensional macroporous bioactive glass for bone regeneration

Lijun JI, Yunfeng SI, Ailing LI, Wenjun WANG, Dong QIU, Aiping ZHU

《化学科学与工程前沿（英文）》 2012年第6卷第4期页码 470-483 doi: 10.1007/s11705-012-1217-1

摘要： Bioactive glasses (BGs) are ideal materials for macroporous scaffolds due to their excellent osteoconductive, osteoinductive, biocompatible and biodegradable properties, and their high bone bonding rates. Macroporous scaffolds made from BGs are in high demand for bone regeneration because they can stimulate vascularized bone ingrowth and they enhance bonding between scaffolds and surrounding tissues. Engineering BG/biopolymers (BP) composites or hybrids may be a good way to prepare macroporous scaffolds with excellent properties. This paper summarizes the progress in the past few years in preparing three-dimensional macroporous BG and BG/BP scaffolds for bone regeneration. Since the brittleness of BGs is a major problem in developing macroporous scaffolds and this limits their use in load bearing applications, the mechanical properties of macroporous scaffolds are particularly emphasized in this review.

关键词： bioactive glass biopolymer bone regeneration macroporous scaffolds tissue engineering

HTML PDF 收藏

Thermochemical pretreatment of meat and bone meal and its effect on methane production

Guangxue WU , Zhenhu HU , Mark G. HEALY , Xinmin ZHAN ,

《环境科学与工程前沿（英文）》 2009年第3卷第3期页码 300-306 doi: 10.1007/s11783-009-0031-6

摘要： Since the solubilization of meat and bone meal (MBM) is a prerequisite in many MBM disposal approaches, enhancement of the solubilization by means of thermochemical pretreatment was investigated in this study at two temperatures (55°C and 131°C) and six sodium hydroxide (NaOH) concentrations (0, 1.25, 2.5, 5, 10 and 20g/L). The MBM volatile solid (VS) reduction ratio was up to 66% and 70% at 55°C and 131°C, respectively. At the same temperature, the VS reduction ratio increased with the increase in the dosage of NaOH. The study on the methane (CH) production potential of pretreated MBM shows that the addition of NaOH at 55°C did not cause the inhibition of the succeeding CH production process. However, CH production was inhibited by the addition of NaOH at 131°C. The CH production potential was in the range of 389 to 503mL CH/g VS MBM and 464 to 555mL CH/g VS MBM at 55°C and 131°C, respectively.

关键词： thermochemical pretreatment meat and bone meal solid reduction solid solubilization methane production potential

HTML PDF 收藏

Bone metastasis of hepatocellular carcinoma: facts and hopes from clinical and translational perspectives

《医学前沿（英文）》 2022年第16卷第4期页码 551-573 doi: 10.1007/s11684-022-0928-z

摘要： Patients with hepatocellular carcinoma (HCC) and bone metastasis (BM) suffer from greatly reduced life quality and a dismal prognosis. However, BM in HCC has long been overlooked possibly due to its relatively low prevalence in previous decades. To date, no consensus or guidelines have been reached or formulated for the prevention and management of HCC BM. Our narrative review manifests the increasing incidence of HCC BM to sound the alarm for additional attention. The risk factors, diagnosis, prognosis, and therapeutic approaches of HCC BM are detailed to provide a panoramic view of this disease to clinicians and specialists. We further delineate an informative cancer bone metastatic cascade based on evidence from recent studies and point out the main factors responsible for the tumor-associated disruption of bone homeostasis and the formation of skeletal cancer lesions. We also present the advances in the pathological and molecular mechanisms of HCC BM to shed light on translational opportunities. Dilemmas and challenges in the treatment and investigation of HCC BM are outlined and discussed to encourage further endeavors in the exploration of underlying pathogenic and molecular mechanisms, as well as the development of novel effective therapies for HCC patients with BM.

关键词： HCC bone osteotropism clinical basic researches advances

HTML PDF 收藏

FGF23 associated bone diseases

null

《医学前沿（英文）》 2013年第7卷第1期页码 65-80 doi: 10.1007/s11684-013-0254-6

摘要：

Recently, fibroblast growth factor 23 (FGF23) has sparked widespread interest because of its potential role in regulating phosphate and vitamin D metabolism. In this review, we summarized the FGF superfamily, the mechanism of FGF23 on phosphate and vitamin D metabolism, and the FGF23 related bone disease.

关键词： fibroblast growth factor 23 FGF receptor phosphate metabolism Klotho bone disease

HTML PDF 收藏

Identification of differentially expressed miRNAs associated with chronic kidney disease–mineral bone

null

《医学前沿（英文）》 2017年第11卷第3期页码 378-385 doi: 10.1007/s11684-017-0541-8

摘要：

The purpose of this study is to characterize a meta-signature of differentially expressed mRNA in chronic kidney disease (CKD) to predict putative microRNA (miRNA) in CKD–mineral bone disorder (CKD–MBD) and confirm the changes in these genes and miRNA expression under uremic conditions by using a cell culture system. PubMed searches using MeSH terms and keywords related to CKD, uremia, and mRNA arrays were conducted. Through a computational analysis, a meta-signature that characterizes the significant intersection of differentially expressed mRNA and expected miRNAs associated with CKD–MBD was determined. Additionally, changes in gene and miRNA expressions under uremic conditions were confirmed with human Saos-2 osteoblast-like cells. A statistically significant mRNA meta-signature of upregulated and downregulated mRNA levels was identified. Furthermore, miRNA expression profiles were inferred, and computational analyses were performed with the imputed microRNA regulation based on weighted ranked expression and putative microRNA targets (IMRE) method to identify miRNAs associated with CKD occurrence. TLR4 and miR-146b levels were significantly associated with CKD–MBD. TLR4 levels were significantly downregulated, whereas pri-miR-146b and miR-146b were upregulated in the presence of uremic toxins in human Saos-2 osteoblast-like cells. Differentially expressed miRNAs associated with CKD-MBD were identified through a computational analysis, and changes in gene and miRNA expressions were confirmed with an in vitro cell culture system.

关键词： chronic kidney disease microRNA mineral bone disorder uremia

HTML PDF 收藏

Factors influencing peak bone mass gain

Xiaowei Zhu, Houfeng Zheng

《医学前沿（英文）》 2021年第15卷第1期页码 53-69 doi: 10.1007/s11684-020-0748-y

摘要： Bone mass is a key determinant of osteoporosis and fragility fractures. Epidemiologic studies have shown that a 10% increase in peak bone mass (PBM) at the population level reduces the risk of fracture later in life by 50%. Low PBM is possibly due to the bone loss caused by various conditions or processes that occur during adolescence and young adulthood. Race, gender, and family history (genetics) are responsible for the majority of PBM, but other factors, such as physical activity, calcium and vitamin D intake, weight, smoking and alcohol consumption, socioeconomic status, age at menarche, and other secondary causes (diseases and medications), play important roles in PBM gain during childhood and adolescence. Hence, the optimization of lifestyle factors that affect PBM and bone strength is an important strategy to maximize PBM among adolescents and young people, and thus to reduce the low bone mass or osteoporosis risk in later life. This review aims to summarize the available evidence for the common but important factors that influence bone mass gain during growth and development and discuss the advances of developing high PBM.

关键词： peak bone mass children adolescents genetic risk factors

HTML PDF 收藏

Bone regeneration by stem cell and tissue engineering in oral and maxillofacial region

Zhiyuan Zhang

《医学前沿（英文）》 2011年第5卷第4期页码 401-413 doi: 10.1007/s11684-011-0161-7

摘要： Clinical imperatives for the reconstruction of jaw bone defects or resorbed alveolar ridge require new therapies or procedures instead of autologous/allogeneic bone grafts. Regenerative medicine, based on stem cell science and tissue engineering technology, is considered as an ideal alternative strategy for bone regeneration. In this paper, we review the current choices of cell source and strategies on directing the osteogenic differentiation of stem cells. The preclinical animal models for bone regeneration and the key translational points to clinical success in oral and maxillofacial region are also discussed. We propose comprehensive strategies based on stem cell and tissue engineering researches, allowing for clinical application in oral and maxillofacial region.

关键词： bone regeneration animal models translational strategies oral and maxillofacial region

HTML PDF 收藏

Measurement of bone alkaline phosphatase and relative study with osteosarcoma

YANG Zhiping, LI Jianmin, LI Xin, HUO Yanqing, SUN Guangzhi

《医学前沿（英文）》 2007年第1卷第1期页码 54-57 doi: 10.1007/s11684-007-0011-9

摘要： The objective of this paper is to explore the value of bone alkaline phosphatase (BALP) for diagnosing osteosarcoma, evaluating the effect of the chemotherapy, judging the prognosis and supervising the relapse and metastasis. The immunoassay was used to check the BALP of the blood serum that was from 42 primary osteosarcoma patients. Alkaline phosphatase (ALP) in blood serum was checked with auto biochemistry equipment. The biopsy tissue and the lesion resected in operation were treated with pathology and histological response was counted. The patients were followed up from five months to 49 months with an average of 24.3 months. Eighteen cases relapsed and transferred, among which, 16 of them were dead, and others were survival to the end of the follow-up. BALP was more sensitive than ALP in diagnosing osteosarcoma ( = 0.015). Fifteen cases decreased to normal value in ALP after preoperative chemotherapy, and 34 cases decreased in BALP. Both ALP and BALP in all cases decreased to normal value in postoperative. There was significant difference in positive correlation between the decrease of BALP and the increase of histological response ( = 0.001, = 0.642). In the followup, there was significant difference in BALP between the group of relapse and transfer and the group of free disease survival ( = 0.000). As a check marker in blood serum, BALP, reflecting the process of ossification, has a higher sensitivity than ALP. It has applied value in the diagnosis of osteosarcoma, reflection of the effect of chemotherapy and forecast the prognosis.

HTML PDF 收藏

Effects of exercise therapy on bone mineral density in early postmenopausal women: a controlled trial

Shilin DENG MD ,

《医学前沿（英文）》 2009年第3卷第3期页码 323-329 doi: 10.1007/s11684-009-0061-2

摘要： The purpose of this article is to determine the effect of a well-designed combined aerobic, resistance, and extension exercise program on bone mineral density (BMD) in postmenopausal women. The population comprised 45 postmenopausal women, who exercised over 12months (exercise group), and 36 women who served as a non-training control group. BMD of the hip, and lumbar spine was measured at the baseline and 12th month. Repeated measurement analysis of variance and nonparametric test were utilized to compare differences between the exercise group and controls. Thirty-six out of 45 persons in the exercise group and 36 controls completed the study. Average compliance was 82.2% for the whole exercise group at the 12th month. All the subjects had decreased BMD, but the rate of bone loss was lower in the exercise group than in the control group at the L4 and hip. Although the exercise program in this study may probably reduce the rate of bone loss in weight-bearing skeletal sites, we do not suggest the exercise by itself be viewed as prevention or treatment for osteoporosis. Further, the exact dose-response relationship of exercise and bone mass in early postmenopause is not clear.

关键词： early postmenopausal women bone mineral density exercise effects

HTML PDF 收藏

Primary cilia in hard tissue development and diseases

《医学前沿（英文）》 2021年第15卷第5期页码 657-678 doi: 10.1007/s11684-021-0829-6

摘要： Bone and teeth are hard tissues. Hard tissue diseases have a serious effect on human survival and quality of life. Primary cilia are protrusions on the surfaces of cells. As antennas, they are distributed on the membrane surfaces of almost all mammalian cell types and participate in the development of organs and the maintenance of homeostasis. Mutations in cilium-related genes result in a variety of developmental and even lethal diseases. Patients with multiple ciliary gene mutations present overt changes in the skeletal system, suggesting that primary cilia are involved in hard tissue development and reconstruction. Furthermore, primary cilia act as sensors of external stimuli and regulate bone homeostasis. Specifically, substances are trafficked through primary cilia by intraflagellar transport, which affects key signaling pathways during hard tissue development. In this review, we summarize the roles of primary cilia in long bone development and remodeling from two perspectives: primary cilia signaling and sensory mechanisms. In addition, the cilium-related diseases of hard tissue and the manifestations of mutant cilia in the skeleton and teeth are described. We believe that all the findings will help with the intervention and treatment of related hard tissue genetic diseases.

关键词： primary cilia bone mechanical sensing hard tissue cilium-related bone disease tooth