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Comments on this special issue on “Wheat Genetics and Breeding”

Zhonghu HE, Xu LLU

《农业科学与工程前沿（英文）》 2019年第6卷第3期页码 309-309 doi: 10.15302/J-FASE-2019264

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Highlights of special issue on “Wheat Genetics and Breeding”

Zhonghu HE, Zhendong ZHAO, Shunhe CHENG

《农业科学与工程前沿（英文）》 2019年第6卷第3期页码 207-209 doi: 10.15302/J-FASE-2019263

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Genomics and genetic breeding in aquatic animals: progress and prospects

Wenteng XU, Songlin CHEN

《农业科学与工程前沿（英文）》 2017年第4卷第3期页码 305-318 doi: 10.15302/J-FASE-2017154

摘要： Genomics focuses on dissection of genome structure and function to provide a molecular basis for understanding the genetic background. In a pivotal step, the expense of whole genome sequencing has been largely eliminated by the rapid updating of sequencing technology, leading to increasing numbers of decoded genomes of aquatic organisms, driving the aquaculture industry into the genomic era. Multiple aquatic areas have been influenced by these findings, such as accelerated generation shift in the seed industry and the process of breeding improved lines. In this article, we have summarized the latest domestic and international progress of aquatic animals in nine aspects, including WGS and fine mapping, construction of high density genetic/physical maps, trait-related marker/genes screening, as well as sex control, genome editing, and other molecular breeding technologies. Finally, the existing problems in this field have been discussed and five future counter measures have been proposed accordingly.

关键词： aquaculture genomics genetic breeding progress

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Genetic study and molecular breeding for high phosphorus use efficiency in maize

null

《农业科学与工程前沿（英文）》 2019年第6卷第4期页码 366-379 doi: 10.15302/J-FASE-2019278

摘要：

Phosphorus is the second most important macronutrient after nitrogen and it has many vital functions in the life of plants. Most soils have a low available P content, which has become a key limiting factor for increasing crop production. Also, low P use efficiency (PUE) of crops in conjunction with excessive application of P fertilizers has resulted in serious environmental problems. Thus, dissecting the genetic architecture of crop PUE, mining related quantitative trait loci (QTL) and using molecular breeding methods to improve high PUE germplasm are of great significance and serve as an efficient approach for the development of sustainable agriculture. In this review, molecular and phenotypic characteristics of maize inbred lines with high PUE, related QTL and genes as well as low-P responses are summarized. Based on this, a breeding strategy applying genomic selection as the core, and integrating the existing genetic information and molecular breeding techniques is proposed for breeding high PUE maize inbred lines and hybrids.

关键词： maize phosphorus use efficiency quantitative trait loci genetic study molecular breeding genomic selection

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Wheat research and breeding in the new era of a high-quality reference genome

Rudi APPELS

《农业科学与工程前沿（英文）》 2019年第6卷第3期页码 225-232 doi: 10.15302/J-FASE-2019265

摘要：

The publications of the International Wheat Genome Sequencing Consortium (IWGSC) released in August 2018 are reviewed and placed into the context of developments arising from the availability of the high-quality wheat genome assembly.

关键词： assembly technology molecular markers polyploidy transcript networks wheat genome

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油菜转基因育种研究进展

官春云

《中国工程科学》 2002年第4卷第8期页码 34-39

摘要：

简要介绍了国外油菜转基因育种情况，包括转除草剂抗性基因，病原菌抗性基因，耐重金属基因，影响种子贮藏产物基因，影响繁殖特性基因，医药和工业产品基因，以及启动子调节基因等。较详细介绍了湖南农业大学在Bt毒蛋白基因转化甘蓝型油菜育成抗虫新品系、基因工程雄性不育系、恢复系选育和杂种优势利用，以及反义FAD2基因转化甘蓝型油菜双低品种提高油酸含量的研究结果；浙江省农科院利用反义PEP基因转化甘蓝型油菜提高种子含油量的研究结果。

关键词：油菜转基因育种

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Breeding for the resistance to

Hongxiang MA, Xu ZHANG, Jinbao YAO, Shunhe CHENG

《农业科学与工程前沿（英文）》 2019年第6卷第3期页码 251-264 doi: 10.15302/J-FASE-2019262

摘要：

With the changes of climate and cultivation systems, the head blight (FHB) epidemic area in China has extended since 2000 from the reaches of the Yangtze River to the north and west winter wheat region. Breeding for FHB resistance in wheat is an effective way to control the disease. Chinese wheat breeders commenced research on FHB in the 1950s. Sumai 3, Ning 7840, Yangmai 158, Ningmai 9 and other cultivars with improved FHB resistance were developed through standard breeding methods and widely applied in production or breeding programs. In addition to intervarietal crosses, alien germplasm was used to improve FHB resistance of wheat. Addition, substitution and translocation lines with alien chromosomes or chromosome fragments were created to enhance FHB resistance. Somaclonal variation was also used to develop a FHB resistant cv. Shengxuan 3 and other cultivars with moderate resistance to FHB were released by such methods. QTL (quantitative trait loci) for FHB resistance were characterized in cultivars originating from China. The major QTL, , was identified on chromosome 3BS in Sumai 3, Ning 894037, Wangshuibai and other Chinese resistant sources. Diagnostic molecular markers for 1 have been applied in wheat breeding and breeding lines with improved FHB resistance and desirable agronomic traits have been obtained. However, breeding for FHB resistance is a long-term task, new technologies are likely to increase the efficiency of this process and better FHB resistance of new cultivars is expected to be achieved within the next decade.

关键词： breeding Fusarium head blight wheat

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Breeding strategies for increasing yield potential in super hybrid rice

Shihua CHENG,Xiaodeng ZHAN,Liyong CAO

《农业科学与工程前沿（英文）》 2015年第2卷第4期页码 277-282 doi: 10.15302/J-FASE-2015081

摘要： Super hybrid rice breeding is a new breeding method combining semi-dwarf breeding and heterosis breeding using germplasm and gene-environment interactions. This paper reviews the breeding strategies of super hybrid rice breeding in China, focusing on the utilization of heterosis of indica and japonica subspecies, construction of ideal plant architecture and pyramiding of disease resistant genes in restorer lines. To develop super hybrid rice, considerable effort should be made to explore genes related with high yield, good quality, resistance to pests and diseases, tolerance to stresses. Molecular breeding methods in combination with crossing techniques should be adopted in super hybrid rice breeding.

关键词： super hybrid rice breeding strategies yield potential

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痩肉猪育种的发展及展望

熊远著

《中国工程科学》 2000年第2卷第9期页码 42-46

摘要：

文章对世界的猪育种过程特别是中国的瘦肉猪育种，从猪种资源调査到优良猪种引进与新品种选育，专门化品系选育与配套利用，种猪测定等方面进行了回顾，就分子生物技术在猪遗传育种中的应用及取得的成果作了简要概述。展望了21世纪瘦肉猪育种的目标，并就新品种培育、猪种资源保存、完善种猪测定体系和加速分子生物技术在猪育种中的应用提出了建议。

关键词：猪瘦肉型育种

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Livestock breeding for the 21st century: the promise of the editing revolution

Chris PROUDFOOT, Gus MCFARLANE, Bruce WHITELAW, Simon LILLICO

《农业科学与工程前沿（英文）》 2020年第7卷第2期页码 129-135 doi: 10.15302/J-FASE-2019304

摘要：

In recent years there has been a veritable explosion in the use of genome editors to create site-specific changes, both and , to the genomes of a multitude of species for both basic research and biotechnology. Livestock, which form a vital component of most societies, are no exception. While selective breeding has been hugely successful at enhancing some production traits, the rate of progress is often slow and is limited to variants that exist within the breeding population. Genome editing provides the potential to move traits between breeds, in a single generation, with no impact on existing productivity or to develop phenotypes that tackle intractable issues such as disease. As such, genome editors provide huge potential for ongoing livestock development programs in light of increased demand and disease challenge. This review will highlight some of the more notable agricultural applications of this technology in livestock.

关键词： cattle pig sheep chicken aquaculture CRISPR

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水稻eui种质的遗传评价和育种利用

杨仁崔

《中国工程科学》 2005年第7卷第8期页码 26-30

摘要：

发现水稻节间伸长的eui新基因及其高秆隐性种质的多型性，育成长穗颈的雄性不育系，组配成e(eui)-杂交稻，并已进入生产应用。e-杂交稻研究和发展的目标是：减少杂交稻种子生产中50%～90%的赤霉素用量，并提高种子产量和质量；杂交稻增产，同时减少杂交稻种植的氮肥用量。已克隆分离出EUI1，EUI2基因，并获得该突变基因碱基缺失多样性的材料，将为植物生长发育，GA代谢激素与杂种优势，以及基因突变的分子机理和植物基因工程的研究提供材料和机遇。

关键词：水稻 eui e-杂交稻遗传评价育种

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Plant genetic engineering and genetically modified crop breeding: history and current status

Xingchun WANG,Shujun CHANG,Jie LU,Rupert FRAY,Don GRIERSON,Yuanhuai HAN

《农业科学与工程前沿（英文）》 2017年第4卷第1期页码 5-27 doi: 10.15302/J-FASE-2017131

摘要： This review charts the major developments in the genetic manipulation of plant cells that have taken place since the first gene transfer experiments using Ti plasmids in 1983. Tremendous progress has been made in both our scientific understanding and technological capabilities since the first genetically modified (GM) crops were developed with single gene resistances to herbicides, insects, viruses, and the silencing of unde-sirable genes. Despite opposition in some parts of the world, the area planted with first generation GM crops has grown from 1.7 Mhm in 1996 to 179.7 Mhm hectares in 2015. The toolkit available for genetic modification has expanded greatly since 1996 and recently Nobel Laureates have called on Greenpeace to end their blanket opposition, and plant scientists have urged that consideration be given to the benefits of GM crops based on actual evidence. It is now possible to use GM to breed new crop cultivars resistant to a much wider range of pests and diseases, and to produce crops better able to adapt to climate change. The advent of new CRISPR-based technologies makes it possible to contemplate a much wider range of improvements based on transfer of new metabolic pathways and traits to improve nutritional quality, with a much greater degree of precision. Use of GM, sometimes in conjunction with other approaches, offers great opportunities for improving food quality, safety, and security in a changing world.

关键词： plant transformation transgene genetic modification

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水产遗传育种与水产种业发展战略研究

桂建芳,包振民,张晓娟

《中国工程科学》 2016年第18卷第3期页码 8-14 doi: 10.15302/J-SSCAE-2016.03.002

摘要：

20多年来，随着水生生物学和生物技术的发展，我国在水产遗传育种与种业方面取得了诸多进展，但也面临着机遇和挑战。本文围绕种质资源保存与利用、遗传机制解析与功能基因挖掘、优良性状新品种选育、水产种业建设等，开展国内外遗传育种现状对比分析研究，分析了当前存在的一些问题，提出未来特别是“十三五”期间水产遗传育种科技发展目标和重点任务。

关键词：水产养殖水产遗传育种水产种业

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Mutagenesis and selective breeding of a high producing

Tian WANG, Shiru JIA, Zhilei TAN, Yujie DAI, Shuai SONG, Guoliang WANG

《化学科学与工程前沿（英文）》 2012年第6卷第2期页码 179-183 doi: 10.1007/s11705-012-1273-6

摘要： -Poly- -lysine ( -PL) is an -lysine linear homopolymer, which is produced by bacteria belonging to the family and by ergot fungi. However, the production of -PL by the wild bacteria strain is very low, which limits its utilization. In most bacteria including the genus, -lysine is a precursor of -PL and is biosynthesized by the -aspartate pathway. Aspartokinase (Ask) is the first key enzyme in this pathway and is subject to complex regulation such as the feedback inhibition by the end product amino acids. In addition, phosphoenolpyruvate carboxykinase is feedback-regulated by -aspartate. To reduce these feedback inhibitions and to improve -PL productivity, resistant mutants were produced using sulfaguanidine (SG) + glycine+ -lysine+ -3-hydroxynorvaline (AHV) as selective markers. Using the interaction between -PL and the charged dye in the solid culture medium, hundreds of colonies were simultaneously screened in a quick and effective manner. Finally, one -PL-producing strain, L9, was selected. The productivity of this strain during flask fermentation was 0.77 g/L, which was 15% higher than that of the original strain. Moreover, its fermentation performance and genetic characteristics were very stable.

关键词： ?-poly-L-lysine plasma AHV Streptomyces diastatochromogenes fermentation

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水稻超高产育种研究进展与前景

陈温福,徐正进,张龙步,张文忠,杨守仁

《中国工程科学》 2002年第4卷第1期页码 31-35

摘要：

水稻单产经历了由矮化育种和杂交稻带来的两次飞跃以后，长时间停滞不前。研究表明，以籼粳稻亚种间强优势利用与理想株型相结合为主要特征的超高产育种（即超级稻），正孕育着单产水平的第3次突破。基本理论与技术路线是增加生物产量，优化产量结构，利用籼粳稻亚远缘杂交或地理远缘杂交创造新株型和强优势，通过优化性状组配使理想株型与优势相结合。按照这一理论与技术路线，已经成功地创造出一批新株型优异种质，并育成了单产12～13 t/hm²的超级稻。

关键词：水稻新株型超高产育种