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The role of lipids in fermentative propionate production from the co-fermentation of lipid and food waste

《环境科学与工程前沿(英文)》 2023年 第17卷 第7期 doi: 10.1007/s11783-023-1686-0

摘要:

● Lipid can promote PA production on a target from food waste.

关键词: Acidogenic fermentation     Microbial community     Volatile fatty acid     Propionate     Food waste     Lipid    

Co-fermentation of waste activated sludge with food waste for short-chain fatty acids production: effect

Leiyu FENG, Yuanyuan YAN, Yinguang CHEN

《环境科学与工程前沿(英文)》 2011年 第5卷 第4期   页码 623-632 doi: 10.1007/s11783-011-0334-2

摘要: Effect of pH ranging from 4.0 to 11.0 on co-fermentation of waste activated sludge (WAS) with food waste for short-chain fatty acids (SCFAs) production at ambient temperature was investigated in this study. Experimental results showed that the addition of food waste significantly improved the performance of WAS fermentation system, which resulted in the increases of SCFAs production and substrate reduction. The SCFAs production at pH 6.0, 7.0, 8.0, and 9.0 and fermentation time of 4 d was respectively 5022.7, 6540.5, 8236.6, and 7911.7 mg COD·L , whereas in the blank tests (no pH adjustment, pH 8.0 (blank test 1), no food waste addition, pH 8.0 (blank test 2), and no WAS addition (blank test 3)) it was only 1006.9, 971.1, and 1468.5 mg COD·L , respectively. The composition of SCFAs at pH from 6.0 to 9.0 was also different from other conditions and propionic acid was the most prevalent SCFA, which was followed by acetic and n-butyric acids, while acetic acid was the top product under other conditions. At pH 8.0 a higher volatile suspended solids (VSS) reduction of 16.6% for the mixture of WAS and food waste than the sole WAS indicated a synergistic effect existing in fermentation system with WAS and food waste. The influence of pH on the variations of nutrient content was also studied during anaerobic fermentation of the mixture of WAS and food waste at different pH conditions. The release of increased with fermentation time at all pH values investigated except 4.0, 5.0 and in blank test one. The concentrations of soluble phosphorus at acidic pHs and in the blank test one were higher than those obtained at alkaline pHs. Ammonia and phosphorus need to be removed before the SCFAs-enriched fermentation liquid from WAS and food waste was used as the carbon source.

关键词: waste activated sludge (WAS)     food waste     co-fermentation     short-chain fatty acids (SCFAs)     pH     synergistic effect    

Upgrading VFAs bioproduction from waste activated sludge via co-fermentation with soy sauce residue

Yanqing Duan, Aijuan Zhou, Kaili Wen, Zhihong Liu, Wenzong Liu, Aijie Wang, Xiuping Yue

《环境科学与工程前沿(英文)》 2019年 第13卷 第1期 doi: 10.1007/s11783-019-1086-7

摘要:

SSR addition upgraded VFAs production from WAS.

Structure modification by pretreatments led to performance distinctions.

Distinctions in microbial community was observed by pretreatments selection.

Up to 0.49‒0.65 billion €/year of market value potential was preliminary estimated.

关键词: Waste activated sludge (WAS)     Soy sauce residue (SSR)     Sludge conditioning     Volatile fatty acids (VFAs)     Microbial diversity    

Effects of Co2+ on the erythromycin biosynthesis

DU Wen, CHEN Changhua

《化学科学与工程前沿(英文)》 2007年 第1卷 第3期   页码 246-250 doi: 10.1007/s11705-007-0044-2

摘要: Erythromycin biosynthesis is a highly complicated process, which involves both primary metabolism and secondary metabolism. The specific activities of the key enzymes related to glucose metabolism such as hexose kinase (HK), glucose-6-phosphate dehydrogenase (6-PDH), phosphofructokinase (PFK), and isocitrate dehydrogenase (ICD), were determined in . The specific activities of the enzymes involved in secondary metabolism, such as methylmalonyl-coenzyme A mutase (MCM) and methylmalonyl-coenzyme A transcarboxylase (MCT), were detected as well. Some organic acids contained in fermentation broth were also analyzed. The results show that Co is able to increase erythromycin biosynthesis. It maybe due to Co improving the specific activities of methylmalonyl-coenzyme A mutase and methylmalonyl-coenzyme A transcarboxylase. Meanwhile, it also enhances the flux of the glucose metabolism pathway.

关键词: secondary metabolism     organic     fermentation     process     phosphofructokinase    

Simultaneous saccharification and fermentation of wheat bran flour into ethanol using coculture of amylotic

K. Manikandan, T. Viruthagiri

《化学科学与工程前沿(英文)》 2009年 第3卷 第3期   页码 240-249 doi: 10.1007/s11705-009-0205-6

摘要: Studies on simultaneous saccharification and fermentation (SSF) of wheat bran flour, a grain milling residue as the substrate using coculture method were carried out with strains of starch digesting and nonstarch digesting and sugar fermenting in batch fermentation. Experiments based on central composite design (CCD) were conducted to maximize the glucose yield and to study the effects of substrate concentration, pH, temperature, and enzyme concentration on percentage conversion of wheat bran flour starch to glucose by treatment with fungal α-amylase and the above parameters were optimized using response surface methodology (RSM). The optimum values of substrate concentration, pH, temperature, and enzyme concentration were found to be 200 g/L, 5.5, 65°C and 7.5 IU, respectively, in the starch saccharification step. The effects of pH, temperature and substrate concentration on ethanol concentration, biomass and reducing sugar concentration were also investigated. The optimum temperature and pH were found to be 30°C and 5.5, respectively. The wheat bran flour solution equivalent to 6% ( / ) initial starch concentration gave the highest ethanol concentration of 23.1 g/L after 48 h of fermentation at optimum conditions of pH and temperature. The growth kinetics was modeled using Monod model and Logistic model and product formation kinetics using Leudeking-Piret model. Simultaneous saccharificiation and fermentation of liquefied wheat bran starch to bioethanol was studied using coculture of amylolytic fungus and nonamylolytic sugar fermenting .

关键词: simultaneous saccharification and fermentation (SSF)     starch     coculture fermentation     statistical experimental design     bioethanol     Monod model    

Repeated batch fermentation with water recycling and cell separation for microbial lipid production

Yumei WANG, Wei LIU, Jie BAO

《化学科学与工程前沿(英文)》 2012年 第6卷 第4期   页码 453-460 doi: 10.1007/s11705-012-1210-8

摘要: Large waste water disposal was the major problem in microbial lipid fermentation because of low yield of lipid. In this study, the repeated batch fermentation was investigated for reducing waste water generated in the lipid fermentation of an oleaginous yeast CX1 strain. The waste fermentation broth was recycled in the next batch operation after the cells were separated using two different methods, centrifugation and flocculation. Two different sugar substrates, glucose and inulin, were applied to the proposed operation. The result showed that at least 70% of the waste water was reduced, while lipid production maintained satisfactory in the initial four cycles. Furthermore, it is suggested that CX1 cells might produce certain naturally occurring inulin hydrolyzing enzyme(s) for obtaining fructose and glucose from inulin directly. Our method provided a practical option for reducing the waste water generated from microbial lipid fermentation.

关键词: batch fermentation     microbial lipid     Trichosporon cutaneum CX1     flocculation     waste water recycle    

Simultaneous saccharification and fermentation of sweet potato powder for the production of ethanol under

Yinxiu CAO, Hongchi TIAN, Kun YAO, Yingjin YUAN

《化学科学与工程前沿(英文)》 2011年 第5卷 第3期   页码 318-324 doi: 10.1007/s11705-010-1026-3

摘要: Due to its merits of drought tolerance and high yield, sweet potatoes are widely considered as a potential alterative feedstock for bioethanol production. Very high gravity (VHG) technology is an effective strategy for improving the efficiency of ethanol fermentation from starch materials. However, this technology has rarely been applied to sweet potatoes because of the high viscosity of their liquid mash. To overcome this problem, cellulase was added to reduce the high viscosity, and the optimal dosage and treatment time were 8 U/g (sweet potato powder) and 1 h, respectively. After pretreatment by cellulase, the viscosity of the VHG sweet potato mash (containing 284.2 g/L of carbohydrates) was reduced by 81%. After liquefaction and simultaneous saccharification and fermentation (SSF), the final ethanol concentration reached 15.5% (v/v), and the total sugar conversion and ethanol yields were 96.5% and 87.8%, respectively.

关键词: bioethanol     sweet potato     very high gravity     viscosity reduction     simultaneous saccharification and fermentation    

Biological hydrogen production from organic wastewater by dark fermentation in China: Overview and prospects

Nanqi REN, Wanqian GUO, Bingfeng LIU, Guangli CAO, Jing TANG

《环境科学与工程前沿(英文)》 2009年 第3卷 第4期   页码 375-379 doi: 10.1007/s11783-009-0148-7

摘要: Biological hydrogen production by dark fermentation is an important part of biological hydrogen production technologies. China is a typical developing country that heavily relies on fossil fuels; thus, new, clean, and sustainable energy development turns quite urgent. It is delightful that Chinese government has already drawn up several H2 development policies since 1990s and provided financial aid to launch some H development projects. In this paper, the research status on dark fermentative hydrogen production in China was summarized and analyzed. Subsequently, several new findings and achievements, with some of which transformed into scale-up tests, were highlighted. Moreover, some prospecting coupling processes with dark fermentation of hydrogen production were also proposed to attract more research interests in the future.

关键词: biological hydrogen production     dark fermentation     overview     prospects    

Purification and crystallization of xylitol from fermentation broth of corncob hydrolysates

Jinchao WEI, Qipeng YUAN, Tianxin WANG, Le WANG,

《化学科学与工程前沿(英文)》 2010年 第4卷 第1期   页码 57-64 doi: 10.1007/s11705-009-0295-1

摘要: Xylitol, a five-carbon sugar alcohol, is a valuable sugar substitute, and widely used in the pharmaceutical, odontological and food industry due to its interesting properties. In the past decades, the xylitol industry has grown rapidly and more attention has been focused on xylitol purification, which possesses an important proportion of the whole industry. In our paper, the purification and crystallization of xylitol fermentation broth by biotechnology using corncob hydrolysates as substance were studied. An initial xylitol fermentation broth was decolored with activated carbon (1% M-1, 60°C, 165rpm), desalted with a combination of two ion-exchange resins (732 and D301), and residual sugars were separated with UBK-555(Ca). Then the solution was vacuum-concentrated up to supersaturation (750g/L xylitol). After adding 1% xylitol crystal seeds, the supersaturated solution was cooled to −20°C for 48h. The crystalline xylitol of a regular tetrahedral shape with purity 95% and crystallization yield 60.2% was obtained from the clarified xylitol fermentation broth. An intact, economical and environmental-friendly route of purification and crystallization of xylitol from fermentation of corncob hydrolysates was obtained, and its experimental procedure and data provided a sound basis for large-scale industrial production.

关键词: ion-exchange     activated     supersaturation     tetrahedral     substitute    

Evaluating the impact of sulfamethoxazole on hydrogen production during dark anaerobic sludge fermentation

《环境科学与工程前沿(英文)》 2023年 第17卷 第1期 doi: 10.1007/s11783-023-1607-2

摘要:

● SMX promotes hydrogen production from dark anaerobic sludge fermentation.

关键词: Sulfamethoxazole     Hydrogen production     Dark anaerobic fermentation     Waste activated sludge    

Biological pretreatment of corn stover by solid state fermentation of

Jian ZHANG, Xin REN, Wenqun CHEN, Jie BAO

《化学科学与工程前沿(英文)》 2012年 第6卷 第2期   页码 146-151 doi: 10.1007/s11705-012-1220-6

摘要: Biological pretreatment is a promising way to overcome the biorecalcitrance of cleaving the supermolecular structure of lignocellulose by lignin degrading enzymes from microorganisms. Solid state fermentation of corn stover with the white-rot fungus was carried out and the efficiency of this pretreatment was evaluated. The enzymatic hydrolysis yield reached a maximum when the corn stover was biologically pretreated for nine days, and the hydrolysis yield decreased sharply if the solid state fermentation was carried out for more than nine days. A possible explanation for this sharp decrease is that not only the lignin degrading enzymes (LiP and MnP) were secreted, but also other metabolites, which were toxic or fatal to the hydrolysis enzymes resulting in the lower hydrolysis yield were generated during the prolonged period of biopretreatment. These results are useful to help determine the optimal timing and to understand the lignin structure and degradation mechanism in biological pretreatment processes.

关键词: biological pretreatment     Phanerochaete chrysosporium     solid state fermentation     biorecalcitrance     hydrolysis yield    

Highly effective extraction of oil from soybean by pretreatment of solid-state fermentation with fungi

Xiaojuan NIU, Shuyi QIU, Yuangen WU, Jie YUAN, Yingying XU

《化学科学与工程前沿(英文)》 2011年 第5卷 第1期   页码 122-125 doi: 10.1007/s11705-010-0533-6

摘要: The solid-state prefermentation by (CICC 2377) and (CICC 40536) was employed to increase the oil extraction yield from soybean. The influence of incubation time on oil yield was investigated. The maximum oil yield extracted from the substrate prefermented by for 96 h was 23%, which increased by 47.4% compared with control (15.6%). In the same fermentation conditions, the maximum oil yield extracted from substrate prefermented by was 21.6%, which increased by 38.5% compared with control (15.6%). The quality of soybean oil was not changed obviously by the pretreatment of fermentation with fungi.

关键词: soybean     Aspergillus niger     Aspergillus flavus     solid-state fermentation     extraction of oil    

Detoxification and concentration of corn stover hydrolysate and its fermentation for ethanol production

Qing Li, Yingjie Qin, Yunfei Liu, Jianjun Liu, Qing Liu, Pingli Li, Liqiang Liu

《化学科学与工程前沿(英文)》 2019年 第13卷 第1期   页码 140-151 doi: 10.1007/s11705-018-1714-y

摘要: Environmental and energy concerns have increased interest in renewable energy sources, particularly biofuels. Thus the fermentation of glucose from sulfuric acid-hydrolyzed corn stover for the production of bioethanol has been explored using a combined acid retardation and continuous-effect membrane distillation treatment process. This process resulted in the separation of the sugars and acids from the acid-catalyzed hydrolysate, the removal of most of the fermentation inhibitors from the hydrolysate and the concentration of the detoxified hydrolysate. The recovery rate of glucose from the sugar-acid mixture using acid retardation was greater than 99.12% and the sulfuric acid was completely recovered from the hydrolysate. When the treated corn stover hydrolysate, containing 100 g/L glucose, was used as a carbon source, 43.06 g/L of ethanol was produced with a productivity of 1.79 g/(L?h) and a yield of 86.31%. In the control experiment, where glucose was used as the carbon source these values were 1.97 g/(L?h) and 93.10% respectively. Thus the integration of acid retardation and a continuous-effect membrane distillation process are effective for the production of fuel ethanol from corn stover.

关键词: corn stover     hydrolysate     acid retardation     continuous-effect membrane distillation     ethanol fermentation    

Strain and process engineering toward continuous industrial fermentation

《化学科学与工程前沿(英文)》 2023年 第17卷 第10期   页码 1336-1353 doi: 10.1007/s11705-022-2284-6

摘要: Most current biotechnology industries are based on batch or fed-batch fermentation processes, which often show low productivity and high production costs compared to chemical processes. To increase the economic competitiveness of biological processes, continuous fermentation technologies are being developed that offer significant advantages in comparison with batch/fed-batch fermentation processes, including: (1) removal of potential substrates and product inhibition, (2) prolonging the microbial exponential growth phase and enhancing productivity, and (3) avoiding repeated fermentation preparation and lowering operation and installation costs. However, several key challenges should be addressed for the industrial application of continuous fermentation processes, including (1) contamination of the fermentation system, (2) degeneration of strains, and (3) relatively low product titer. In this study, we reviewed and discussed metabolic engineering and synthetic biology strategies to address these issues.

关键词: continuous fermentation     productivity     contamination     strain degeneration     metabolic engineering    

Vacuum promotes metabolic shifts and increases biogenic hydrogen production in dark fermentation systems

Haifa RAJHI,Daniel PUYOL,Mirna C. MARTÍNEZ,Emiliano E. DÍAZ,José L. SANZ

《环境科学与工程前沿(英文)》 2016年 第10卷 第3期   页码 513-521 doi: 10.1007/s11783-015-0777-y

摘要: The successful operation of any type of hydrogen-producing bioreactor depends on the performance of the microorganisms present in the system. Both substrate and partial gas pressures are crucial factors affecting dark fermentation metabolic pathways. The main objective of this study was to evaluate the impact of both factors on hydrogen production using anaerobic granular sludge as inoculum and, secondly, to study the metabolic shifts of an anaerobic community subjected to low partial gas pressures. With this goal in mind, seven different wastewater (four synthetic media, two industrial wastewater, and one domestic effluent) and the effect of applying vacuum on the systems were analyzed. The application of vacuum promoted an increase in the diversity of hydrogen-producing bacteria, such as , and promoted the dominance of acetoclastic- over hydrogenotrophic methanogens. The application of different media promoted a wide variety of metabolic pathways. Nevertheless, reduction of the hydrogen partial pressure by application of vacuum lead to further oxidation of reaction intermediates irrespective of the medium used, which resulted in higher hydrogen and methane production, and improved the COD removal. Interestingly, vacuum greatly promoted biogenic hydrogen production from a real wastewater, which opens possibilities for future application of dark fermentation systems to enhance biohydrogen yields.

关键词: dark fermentation     biohydrogen     wastewaters     vacuum    

标题 作者 时间 类型 操作

The role of lipids in fermentative propionate production from the co-fermentation of lipid and food waste

期刊论文

Co-fermentation of waste activated sludge with food waste for short-chain fatty acids production: effect

Leiyu FENG, Yuanyuan YAN, Yinguang CHEN

期刊论文

Upgrading VFAs bioproduction from waste activated sludge via co-fermentation with soy sauce residue

Yanqing Duan, Aijuan Zhou, Kaili Wen, Zhihong Liu, Wenzong Liu, Aijie Wang, Xiuping Yue

期刊论文

Effects of Co2+ on the erythromycin biosynthesis

DU Wen, CHEN Changhua

期刊论文

Simultaneous saccharification and fermentation of wheat bran flour into ethanol using coculture of amylotic

K. Manikandan, T. Viruthagiri

期刊论文

Repeated batch fermentation with water recycling and cell separation for microbial lipid production

Yumei WANG, Wei LIU, Jie BAO

期刊论文

Simultaneous saccharification and fermentation of sweet potato powder for the production of ethanol under

Yinxiu CAO, Hongchi TIAN, Kun YAO, Yingjin YUAN

期刊论文

Biological hydrogen production from organic wastewater by dark fermentation in China: Overview and prospects

Nanqi REN, Wanqian GUO, Bingfeng LIU, Guangli CAO, Jing TANG

期刊论文

Purification and crystallization of xylitol from fermentation broth of corncob hydrolysates

Jinchao WEI, Qipeng YUAN, Tianxin WANG, Le WANG,

期刊论文

Evaluating the impact of sulfamethoxazole on hydrogen production during dark anaerobic sludge fermentation

期刊论文

Biological pretreatment of corn stover by solid state fermentation of

Jian ZHANG, Xin REN, Wenqun CHEN, Jie BAO

期刊论文

Highly effective extraction of oil from soybean by pretreatment of solid-state fermentation with fungi

Xiaojuan NIU, Shuyi QIU, Yuangen WU, Jie YUAN, Yingying XU

期刊论文

Detoxification and concentration of corn stover hydrolysate and its fermentation for ethanol production

Qing Li, Yingjie Qin, Yunfei Liu, Jianjun Liu, Qing Liu, Pingli Li, Liqiang Liu

期刊论文

Strain and process engineering toward continuous industrial fermentation

期刊论文

Vacuum promotes metabolic shifts and increases biogenic hydrogen production in dark fermentation systems

Haifa RAJHI,Daniel PUYOL,Mirna C. MARTÍNEZ,Emiliano E. DÍAZ,José L. SANZ

期刊论文