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期刊论文 3

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2020 1

2012 1

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3D细胞容器 1

相互作用 1

血管生成 1

非接触式多细胞共培养 1

骨生成 1

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Direct ethanol production from rice straw by coculture with two high-performing fungi

Maki TAKANO, Kazuhiro HOSHINO

《化学科学与工程前沿(英文)》 2012年 第6卷 第2期   页码 139-145 doi: 10.1007/s11705-012-1281-6

摘要: To develop efficient and economical direct ethanol production from fine rice straw crashed mechanically, two high-performing fungi, which can secret hyperactive cellulases and/or ferment effectively various sugars, were selected from some strains belong to preserved in our laboratory. The simultaneous saccharification and fermentation (SSF) by coculture with these fungi was investigated. The screening of high-performing fungi resulted in the selection of NBRC 4572 as an ethanol-producing fungus and NBRC 5398 as a cellulase-secreting fungus. The strain 4572 produced ethanol aerobically from glucose and xylose in high yields of 0.420 g/g at 36 h and 0.478 g/g at 60 h, respectively, but secreted fairly low cellulases. On the other hand, the strain 5398 also produced ethanol from glucose in yield of 0.340 g/g though it had a little growth in xylose culture. However, it secreted hyperactive cellulases that are essential for hydrolysis of rice straw in culture and the maximum activities of endo-β-glucanase and β-glucosidase were 2.11 U/L and 1.47 U/L, respectively. In SSF of rice straw by coculture with two fungi selected, the ethanol production reached 1.28 g/L after 96 h when the inoculation ratio of the strain 5398 to the strain 4572 was 9.

关键词: Mucor circinelloides     ethanol production     cellulase secretion     SSF     coculture     rice straw    

Simultaneous saccharification and fermentation of wheat bran flour into ethanol using coculture of amylotic

K. Manikandan, T. Viruthagiri

《化学科学与工程前沿(英文)》 2009年 第3卷 第3期   页码 240-249 doi: 10.1007/s11705-009-0205-6

摘要: Studies on simultaneous saccharification and fermentation (SSF) of wheat bran flour, a grain milling residue as the substrate using coculture method were carried out with strains of starch digesting and nonstarch digesting and sugar fermenting in batch fermentation. Experiments based on central composite design (CCD) were conducted to maximize the glucose yield and to study the effects of substrate concentration, pH, temperature, and enzyme concentration on percentage conversion of wheat bran flour starch to glucose by treatment with fungal α-amylase and the above parameters were optimized using response surface methodology (RSM). The optimum values of substrate concentration, pH, temperature, and enzyme concentration were found to be 200 g/L, 5.5, 65°C and 7.5 IU, respectively, in the starch saccharification step. The effects of pH, temperature and substrate concentration on ethanol concentration, biomass and reducing sugar concentration were also investigated. The optimum temperature and pH were found to be 30°C and 5.5, respectively. The wheat bran flour solution equivalent to 6% ( / ) initial starch concentration gave the highest ethanol concentration of 23.1 g/L after 48 h of fermentation at optimum conditions of pH and temperature. The growth kinetics was modeled using Monod model and Logistic model and product formation kinetics using Leudeking-Piret model. Simultaneous saccharificiation and fermentation of liquefied wheat bran starch to bioethanol was studied using coculture of amylolytic fungus and nonamylolytic sugar fermenting .

关键词: simultaneous saccharification and fermentation (SSF)     starch     coculture fermentation     statistical experimental design     bioethanol     Monod model    

3D 打印细胞容器样支架在多细胞组织工程中的应用研究 Article

王晓亚, 张猛, 马景阁, 徐梦驰, 常江, Michael Gelinsky, 吴成铁

《工程(英文)》 2020年 第6卷 第11期   页码 1276-1284 doi: 10.1016/j.eng.2020.08.001

摘要:

人工设计的非接触式多细胞共培养模型可以模仿人体组织的细胞内微环境,但其发展始终面临各种挑战。本研究利用3D打印技术,成功制得了一种含β-磷酸三钙/羟基磷灰石(β-TCP/HA)的细胞容器样支架,该支架设计了四种不同形状的微孔结构:三角形、正方形、平行四边形和长方形微孔结构。这些支架可以在非接触的方式下同时培养四种细胞。本研究构建了一种由人骨髓间质干细胞(HBMSC)、人脐静脉内皮细胞(HUVEC)、人脐静脉平滑肌细胞(HUVSMC)、人真皮成纤维细胞(HDF)组成的3D共培养模型,用以研究这些细胞在促进骨生成和血管生成过程中细胞的个体效应与协同效应。结果表明,相较于在3D细胞容器中仅培养一种细胞,共培养三种或四种细胞展现出了更高的细胞增殖率。HBMSC与HUVEC的细胞间相互作用的研究表明,含有四种独立空间结构的3D细胞容器可以通过放大共培养细胞的旁分泌效应促进细胞的骨生成和血管生成能力。此外,在3D细胞容器中建立多细胞非接触体系,特别是含有三种或四种细胞的共培养体系,相对于单细胞培养模式与两种细胞共培养模式来说,在促进细胞成骨分化和成血管分化方面展现出明显的优势。本研究为基于支架的多细胞非接触式共培养体系的发展提供了新的研究方向。

关键词: 3D细胞容器     非接触式多细胞共培养     相互作用     血管生成     骨生成    

标题 作者 时间 类型 操作

Direct ethanol production from rice straw by coculture with two high-performing fungi

Maki TAKANO, Kazuhiro HOSHINO

期刊论文

Simultaneous saccharification and fermentation of wheat bran flour into ethanol using coculture of amylotic

K. Manikandan, T. Viruthagiri

期刊论文

3D 打印细胞容器样支架在多细胞组织工程中的应用研究

王晓亚, 张猛, 马景阁, 徐梦驰, 常江, Michael Gelinsky, 吴成铁

期刊论文