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科技自主创新与中西文化互补之我见——六个典型实例的思考

李世煇

《中国工程科学》 2005年 第7卷 第4期   页码 11-21

摘要：

从中西文化互补的角度，考察了20世纪70年代以来中国科技原始性自主创新的六个典型实例，简述了笔者从事典型信息法研究的几点认识，试图以事实论证：中国传统文化对当代科技自主创新有重大作用，并对其表现形式等做了初步考察。

关键词： 中国传统文化     西方文化     互补     科技自主创新     典型实例    

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Key stakeholders’ perspectives on the ideal partnering culture in construction projects

Gunnar J. LÜHR, Marian G. C. BOSCH-REKVELDT, Mladen RADUJKOVIC

《工程管理前沿（英文）》 2022年 第9卷 第2期   页码 312-325 doi: 10.1007/s42524-020-0135-z

摘要： This paper examines the current state of project cultures in the German turnkey construction industry and the ideal project cultures in terms of partnering from the perspective of various key stakeholders (i.e., Investors, General Contractors, (Sub-)Contractors and Designers). To investigate the current and ideal cultures, data were gathered among the key stakeholders by means of a survey study with 72 respondents divided over 12 companies. The respondents rated the current and desired cultures by using the Organizational Culture Assessment Instrument, which belongs to the Competing Values Framework. The investigations show many similarities and differences between the stakeholder perspectives of the current and the idealized partnering project cultures. Mainly, the General Contractors desire more cooperative behaviors than the (Sub-)Contractors, and the Investors desire more pronounced flexibility than the General Contractors. All stakeholders desire a cultural change from highly competitive behaviors toward more cooperation. Changes in terms of clear procedures or more flexibility are only desired by the Designers. Defining both the current and an ideal partnering project culture enables academics and project managers to compare their actual project cultures to an ideal situation. With such an approach, academics and project managers could measure whether new tools or changes in resources affect their project cultures toward a partnering project culture.

关键词： project culture     organisational culture     partnering     construction culture     stakeholder perspectives     German    

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Mixed culture of

Kishore Gopalakrishnan, Javad Roostaei, Yongli Zhang

《环境科学与工程前沿（英文）》 2018年 第12卷 第4期 doi: 10.1007/s11783-018-1075-2

摘要：

RSM is used to explore the impact of different parameter on algal growth response.

Mixed algal culture promotes algal biomass and lipid accumulation.

Optimized conditions achieve maximum productivity of algal biomass and lipid.

关键词： Algal biofuels     Algal mixed cultures     Algal biomass     Algal lipid     Wastewater     Response surface methodology    

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Construction engineering management culture shift: Is the lowest tender offer dead?

Eric SCHEEPBOUWER, Douglas D. GRANSBERG, Carla Lopez del PUERTO

《工程管理前沿（英文）》 2017年 第4卷 第1期   页码 49-57 doi: 10.15302/J-FEM-2017014

摘要： The procurement of public construction projects must walk a fine line between the corruption of state officials and collusion of contractors. The method of awarding projects to the lowest responsible tenderer was originally implemented to guard against corruption of state officials. However, an investigation of the construction industry in the Canadian province of Quebec showed that lowest-tender-offer procurement gave rise to collusion of companies tendering for the contracts. Alternatively, best-value procurement has been used for decades, but here problems arise owing to the necessity of subjective judging of measures other than price to compare bids, giving rise to time- and money-consuming protests. The paper proposes a compelling argument that the construction engineering management (CEM) culture should refocus its efforts on enhancing project cost certainty rather than merely searching for means to design a project in a manner that produces the lowest initial cost, and awards the construction to the lowest tender offer that focuses on cost savings during the project development and delivery process. The difference in the two approaches is subtle but extremely important. To make the transition, the engineering management tools must be advanced to the next level. This means that all project control tools for managing cost, schedule, and technical scope must be transformed from working in the deterministic mode to the stochastic mode, thus making the probability of completing the project within or below its official budget the primary decision criterion. To do so, CEMs must accept that there is a benefit in paying more for an alternative that increases cost certainty for the entire project. The authors of this paper hope that it will provide the grist for a more general dialog across all industry sectors where engineering management is practiced.

关键词： cost certainty     lowest responsible bid     best value     public procurement     construction engineering management culture    

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苏通大桥工程文化建设实践与经验

俞春生,姚蓓,王茜

《中国工程科学》 2009年 第11卷 第3期   页码 92-96

摘要：

在我国大型工程建设管理的过程中审时度势地融入文化的力量，必将为我国工程建设管理注入活力。工程文化是工程内容与文化形式有机统一的综合体，由于工程的时限性与一次性，使得工程文化体系的建设带有特殊性，通过对苏通大桥工程文化体系建设过程的研究，肯定了大型工程文化建设是工程管理的软实力，同时也在深入分析的基础上，对大型工程建设中构建工程文化体系进行了较为系统的思考，包括工程文化内涵的深刻认识、工程文化建设的原则、工程文化建设的内容以及如何有效地使工程文化快速形成和有效渗透等方面，为后续大型工程文化体系的建设提供了参

关键词： 苏通大桥工程文化     大型工程文化体系建设     系统思维    

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Studies on culture condition of new marine bacterium sp. SY01

LAN Wenjian, MO Linfeng, LI Houjin, CAI Chuanghua, ZHOU Yipin, YAO Junhua

《化学科学与工程前沿（英文）》 2008年 第2卷 第4期   页码 443-446 doi: 10.1007/s11705-008-0065-5

摘要： New marine bacterium sp. SY01, producer of prodigiosin, was isolated from the seawaters of Sanya Bay. The culture conditions of this bacterium were investigated. sp. SY01 was cultured in 2216E media which contained tryptophan, histidine, lactonic acid, camphor, limonene, casein, diphenyl guanidine, coumarin and 1,3-dinitrobenzene, respectively. After 5 days cultivation, the extracts of different culture broths were detected by direct infusion mass spectroscopy using positive ESI mode. As the results, tryptophan, histidine and casein didn’t show any observable influences on the biosynthesis of prodigiosin. Lactonic acid, camphor, limonene, diphenyl guanidine, coumarin could inhibit the bacterium growth and prodigiosin biosynthesis to a certain extent, slower the culture broth to turn red. However, 1, 3-dinitrobenzene inhibited the bacteria to produce prodigiosin completely. MS data suggested that various metabolites with chemodiversity were produced in different culture media. In particular, a series of high-molecular-weight compounds with high relative abundances were observed in the medium containing limonene. To further optimize the culture condition, more new prodigiosin analogues and lead compounds can be obtained and the goal of “one strain-many compounds” can be achieved.

关键词： producer     high-molecular-weight     relative     culture     different culture    

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我国先进汽车文化发展研究

俞宁,乔英俊,胡卫国,杨沿平,陈志林,黄威

《中国工程科学》 2018年 第20卷 第1期   页码 139-143 doi: 10.15302/J-SSCAE-2018.01.020

摘要：

我国进入汽车社会和建设汽车强国迫切需要有先进的汽车文化。本文在分析论述了建设先进汽车文化重要性的基础上，对我国汽车文化的现状及存在的问题进行了分析，明确了我国建设先进汽车文化的目标及指导思想，提出了发展先进汽车文化的路径，这些都将有利于我国汽车文化的发展壮大。

关键词： 汽车文化     先进文化     中国特色     发展路径    

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Investigation of polyhydroxyalkanoates (PHAs) biosynthesis from mixed culture enriched by valerate-dominant

Jiuxiao Hao, Xiujin Wang, Hui Wang

《环境科学与工程前沿（英文）》 2017年 第11卷 第1期 doi: 10.1007/s11783-017-0896-8

摘要： Valerate-hydrolysate enriched culture showed great ability to produce 3HV and 3H2MV. Valerate-hydrolysate enriched culture had more in the community. Mixed iso-/n-valerate was effective at balancing microbial growth and PHAs synthesis. Co-substrates of valerate and propionate raised the fraction of 3HV and 3H2MV. The production of polyhydroxyalkanoates (PHAs) with a high fraction of 3-hydroxyvalerate (3HV) and 3-hydroxy-2-methylvalerate (3H2MV) from mixed culture enriched by valerate-dominant hydrolysate was evaluated in this study. After long-term enrichment, the culture showed strong ability to synthesize 3HV and 3H2MV, even with acetate-dominant substrate. The ultilization of single or mixed iso-/n-valerate by the enriched culture showed that the mixture of iso-valerate and n-valerate was more efficient substrate than any single in terms of balancing microbial growth and PHAs synthesis. Besides, through comparing the kinetics and stoichiometry of the tests supplying valerate and propionate, the enriched culture with equivalent valerate and propionate (1:1 molar ratio) exhibited superior PHAs production performances to pure valerate or propionate, attaining more than 70 mol% of 3HV and 3H2MV. The above findings reveal that valerate-dominant hydrolysate is a kind of suitable substrate to enrich PHAs producing culture with great capability to synthesize 3HV and 3H2MV monomers, thus improving product properties than pure poly(3-hydroxybutyrate) (P3HB); also 3HV and 3H2MV production behaviors can be regulated by the type of odd-carbon VFAs in the substrate.

关键词： Polyhydroxyalkanoates (PHAs)     Valerate     Mixed culture     3-hydroxyvalerate (3HV)     Propionate    

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Positive stool culture could predict the clinical outcomes of haploidentical hematopoietic stem cell

Lijuan Hu, Qi Wang, Xiaohui Zhang, Lanping Xu, Yu Wang, Chenhua Yan, Huan Chen, Yuhong Chen, Kaiyan Liu, Hui Wang, Xiaojun Huang, Xiaodong Mo

《医学前沿（英文）》 2019年 第13卷 第4期   页码 492-503 doi: 10.1007/s11684-019-0681-0

摘要： We aimed to identify the effect of positive stool cultures (PSCs) on the clinical outcomes of patients undergoing haploidentical hematopoietic stem cell transplantation (haplo-HSCT) ( = 332). PSCs were observed in 61 patients (PSC group, 18.4%). Enterobacteriaceae in stool specimens was associated with a higher risk of bloodstream infection, and in stool specimens was related to a higher risk of platelet engraftment failure. The cumulative incidence of infection-related mortality 1 year after haplo-HSCT in the PSC group was higher than that of the patients who showed persistently negative stool cultures (NSC group; 19.2% vs. 8.9%, = 0.017). The probabilities of overall survival (71.4% vs. 83.8%, = 0.031) and disease-free survival (69.6% vs. 81.0%, = 0.048) 1 year after haplo-HSCT for the PSC group were significantly lower than those for the NSC group, particularly for patients who had in their stool specimens. In multivariate analysis, in stool specimens significantly increased the risk of mortality and was associated with poorer survival. Our results showed that PSC influenced the clinical outcomes after haplo-HSCT, particularly those who had in their stool specimens.

关键词： haploidentical     hematopoietic stem cell transplantation     stool culture     Candida    

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The rpoS deficiency suppresses acetate accumulation in glucose-enriched culture of <

Prayoga SURYADARMA, Yoshihiro OJIMA, Yuto FUKUDA, Naohiro AKAMATSU, Masahito TAYA

《化学科学与工程前沿（英文）》 2012年 第6卷 第2期   页码 152-157 doi: 10.1007/s11705-012-1287-0

摘要： The role of on the central carbon metabolism was investigated through analyzing the deficiency of this regulon gene under aerobic and glucose-enriched culture conditions. The experimental results showed that while the wild type cells exhibited an overflow metabolism effect, the -deleting mutation alleviated this effect with the significant suppression of acetate accumulation under a high glucose condition. This gene deletion also induced the twofold upregulation of and one-tenth downregulation of , respectively. The overflow metabolism effect was confirmed to be recovered by re-introducing gene into the mutant. These results demonstrated changed the central carbon metabolism toward acetate overflow through dehydrogenation of pyruvate and reduction of TCA cycle activity.

关键词： Escherichia coli     rpoS     aerobic and glucose-enriched culture     overflow metabolism    

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Comparison of human nasal epithelial cells grown as explant outgrowth cultures or dissociated tissue cultures

null

《医学前沿（英文）》 2013年 第7卷 第4期   页码 486-491 doi: 10.1007/s11684-013-0287-x

摘要：

The purpose of this study was to compare cell growth characteristics, ciliated cell differentiation, and function of human nasal epithelial cells established as explant outgrowth cultures or dissociated tissue cultures. Human nasal mucosa of the uncinate process was obtained by endoscopy and epithelial cell cultures were established by explant outgrowth or dissociated tissue culture methods. Epithelial cell growth characteristics were observed by inverted phase contrast microscopy. Ciliated cell differentiation was detected by β-tubulin IV and ZO-1 immunocytochemistry. Basal and ATP-stimulated ciliary beat frequency (CBF) was measured using a high-speed digital microscopic imaging system. Both the explant and dissociated tissue cultures established as monolayers with tight junctions and differentiated cell composition, with both types of cultures comprising ciliated and non-ciliated epithelial cells. Fibroblasts were also frequently found in explant cultures but rarely seen in dissociated tissue cultures. In both culture systems, the highest ciliated cell density appeared at 7th–10th culture day and declined with time, with the lifespan of ciliated cells ranging from 14 to 21 days. Overall, 10% of the cells in explant cultures and 20% of the cells in the dissociated tissue cultures were ciliated. These two cultures demonstrated similar ciliary beat frequency values at baseline (7.78±1.99 Hz and 7.91±2.52 Hz, respectively) and reacted equivalently following stimulation with 100 μM ATP. The results of this study indicate that both the explant outgrowth and dissociated tissue culture techniques are suitable for growing well-differentiated nasal ciliated and non-ciliated cells, which have growth characteristics and ciliary activity similar to those of nasal epithelial cells in vivo.

关键词： ciliated cells     ciliary beat frequency     dissociated tissue culture     explant culture     nasal epithelial cells    

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Statistical modeling and optimization for enhanced hyaluronic acid production by batch culture of Sreptococcus

Long LIU, Haiquan YANG, Guocheng DU, Miao WANG, Jian CHEN,

《化学科学与工程前沿（英文）》 2009年 第3卷 第4期   页码 351-356 doi: 10.1007/s11705-009-0248-8

摘要： This work is aimed to achieve the optimal hyaluronic acid (HA) production by batch culture of via the supplement of nucleotide bases using response surface methodology (RSM). First, the influence of nucleotide bases (adenine, guanine, cytosine, thymine, and uracil) on microbial HA production was investigated using fractional factorial design (FFD). By a 2 FFD, uracil was found to be the most significant factor for cell growth and HA production, while the other nucleotide bases were shown to have no significant effects on cell growth and HA production. Also, the impact of uracil on cell growth and HA production was further investigated by RSM, where two variables were considered: uracil concentration and supplement time. The optimal uracil concentration and supplement time were found to be 0.051g/L and 7h, respectively, and the predicted maximal HA production reached 6.42g/L. The maximal HA production increased from 5.0g/L of the control without uracil supplement to 6.31g/L at the optimal conditions in validation experiments.

关键词： control     microbial HA     thymine     influence     culture    

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Real time monitoring of bioreactor mAb IgG3 cell culture process dynamics via Fourier transform infraredspectroscopy: Implications for enabling cell culture process analytical technology?

Huiquan Wu, Erik Read, Maury White, Brittany Chavez, Kurt Brorson, Cyrus Agarabi, Mansoor Khan

《化学科学与工程前沿（英文）》 2015年 第9卷 第3期   页码 386-406 doi: 10.1007/s11705-015-1533-3

摘要： Compared to small molecule process analytical technology (PAT) applications, biotechnology product PAT applications have certain unique challenges and opportunities. Understanding process dynamics of bioreactor cell culture process is essential to establish an appropriate process control strategy for biotechnology product PAT applications. Inline spectroscopic techniques for real time monitoring of bioreactor cell culture process have the distinct potential to develop PAT approaches in manufacturing biotechnology drug products. However, the use of inline Fourier transform infrared (FTIR) spectroscopic techniques for bioreactor cell culture process monitoring has not been reported. In this work, real time inline FTIR Spectroscopy was applied to a lab scale bioreactor mAb IgG3 cell culture fluid biomolecular dynamic model. The technical feasibility of using FTIR Spectroscopy for real time tracking and monitoring four key cell culture metabolites (including glucose, glutamine, lactate, and ammonia) and protein yield at increasing levels of complexity (simple binary system, fully formulated media, actual bioreactor cell culture process) was evaluated via a stepwise approach. The FTIR fingerprints of the key metabolites were identified. The multivariate partial least squares (PLS) calibration models were established to correlate the process FTIR spectra with the concentrations of key metabolites and protein yield of in-process samples, either individually for each metabolite and protein or globally for all four metabolites simultaneously. Applying the 2 derivative pre-processing algorithm to the FTIR spectra helps to reduce the number of PLS latent variables needed significantly and thus simplify the interpretation of the PLS models. The validated PLS models show promise in predicting the concentration profiles of glucose, glutamine, lactate, and ammonia and protein yield over the course of the bioreactor cell culture process. Therefore, this work demonstrated the technical feasibility of real time monitoring of the bioreactor cell culture process via FTIR spectroscopy. Its implications for enabling cell culture PAT were discussed.

关键词： process analytical technology (PAT)     Fourier-transform infrared (FTIR) spectroscopy     partial least squares (PLS) regression     mouse IgG3     bioreactor cell culture process     real time process monitoring    

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efficient and selective removal of phosphate from wastewater of sea cucumber aquaculture for microalgae culture

《环境科学与工程前沿（英文）》 2023年 第17卷 第10期 doi: 10.1007/s11783-023-1720-2

摘要：

● A new adsorption-membrane separation strategy is used for phosphate removal.

关键词： Adsorption-membrane     Low-concentration phosphate     Zr-modified-bentonite     Recycle     Microalgal culture    

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基于中国传统的项目管理研究

周红,成虎

《中国工程科学》 2006年 第8卷 第2期   页码 85-89

摘要：

项目一次性和中西方文化差异是中国项目管理问题根源所在。项目组织文化和企业文化不同，具有特殊性。讨论了中国儒道释一体的传统文化对项目管理中“软问题”和PMBOK中的“软模块”有深刻的影响。论述了基于中国文化的项目管理应当在沟通、人力资源、合同、管理道德等方面推动理论创新和实践。

关键词： 项目管理     中国传统文化     理论体系    

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标题 作者 时间 类型 操作

科技自主创新与中西文化互补之我见——六个典型实例的思考

李世煇

期刊论文

Key stakeholders’ perspectives on the ideal partnering culture in construction projects

Gunnar J. LÜHR, Marian G. C. BOSCH-REKVELDT, Mladen RADUJKOVIC

期刊论文

Mixed culture of

Kishore Gopalakrishnan, Javad Roostaei, Yongli Zhang

期刊论文

Construction engineering management culture shift: Is the lowest tender offer dead?

Eric SCHEEPBOUWER, Douglas D. GRANSBERG, Carla Lopez del PUERTO

期刊论文

苏通大桥工程文化建设实践与经验

俞春生,姚蓓,王茜

期刊论文

Studies on culture condition of new marine bacterium sp. SY01

LAN Wenjian, MO Linfeng, LI Houjin, CAI Chuanghua, ZHOU Yipin, YAO Junhua

期刊论文

我国先进汽车文化发展研究

俞宁,乔英俊,胡卫国,杨沿平,陈志林,黄威

期刊论文

Investigation of polyhydroxyalkanoates (PHAs) biosynthesis from mixed culture enriched by valerate-dominant

Jiuxiao Hao, Xiujin Wang, Hui Wang

期刊论文

Positive stool culture could predict the clinical outcomes of haploidentical hematopoietic stem cell

Lijuan Hu, Qi Wang, Xiaohui Zhang, Lanping Xu, Yu Wang, Chenhua Yan, Huan Chen, Yuhong Chen, Kaiyan Liu, Hui Wang, Xiaojun Huang, Xiaodong Mo

期刊论文

The rpoS deficiency suppresses acetate accumulation in glucose-enriched culture of <

Prayoga SURYADARMA, Yoshihiro OJIMA, Yuto FUKUDA, Naohiro AKAMATSU, Masahito TAYA

期刊论文

Comparison of human nasal epithelial cells grown as explant outgrowth cultures or dissociated tissue cultures

null

期刊论文

Statistical modeling and optimization for enhanced hyaluronic acid production by batch culture of Sreptococcus

Long LIU, Haiquan YANG, Guocheng DU, Miao WANG, Jian CHEN,

期刊论文

Real time monitoring of bioreactor mAb IgG3 cell culture process dynamics via Fourier transform infraredspectroscopy: Implications for enabling cell culture process analytical technology?

Huiquan Wu, Erik Read, Maury White, Brittany Chavez, Kurt Brorson, Cyrus Agarabi, Mansoor Khan

期刊论文

efficient and selective removal of phosphate from wastewater of sea cucumber aquaculture for microalgae culture

期刊论文

基于中国传统的项目管理研究

周红,成虎

期刊论文