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Progress in studies of sex determination mechanisms and sex control techniques in

Qian ZHOU,Songlin CHEN

《农业科学与工程前沿(英文)》 2016年 第3卷 第2期   页码 113-123 doi: 10.15302/J-FASE-2016093

摘要: The (half-smooth tongue sole) is a marine flatfish of great commercial value for fisheries and aquaculture in China. It has a female heterogametic sex determination system (ZW/ZZ) and environmental factors can induce sex-reversal of females to phenotypic males, suggesting that it is a promising model for the study of sex determination mechanisms. Additionally, females grow much faster than males and it is feasible to improve the aquaculture production through sex control techniques. This paper reviews the progress in research on sex determination mechanisms research in our laboratory. We have completed whole-genome sequencing and revealed the genome organization and sex chromosome evolution of . A putative male determining gene was identified and DNA methylation was verified as having a crucial role in the sex reversal process. Genetic maps and sex-specific biomarkers have been used in a marker-assisted selection breeding program and for differentiation of the fish sex. Development and improvement of sex control technologies, including artificial gynogenesis and production of breeding fry with high proportion of females, is also reviewed. These research advances have provided insight into the regulation of sex determination and enabled efficient sex management in artificial culturing of .

关键词: Cynoglossus semilaevis     sex determination     sex control technique    

Rapid method for on-site determination of phenolic contaminants in water using a disposable biosensor

Yuanting LI, Dawei LI, Wei SONG, Meng LI, Jie ZOU, Yitao LONG

《环境科学与工程前沿(英文)》 2012年 第6卷 第6期   页码 831-838 doi: 10.1007/s11783-012-0393-z

摘要: A disposable biosensor was fabricated using single-walled carbon nanotubes, gold nanoparticles and tyrosinase (SWCNTs-AuNPs-Tyr) modified screen-printed electrodes. The prepared biosensor was applied to the rapid determination of phenolic contaminants within 15 minutes. The SWCNTs-AuNPs-Tyr bionanocomposite sensing layer was characterized with scanning electron microscopy, electrochemical impedance spectroscopy and cyclic voltammetry methods. The characterization results revealed that SWCNTs could lead to a high loading of tyrosinase (Tyr) with the large surface area and the porous morphology, while AuNPs could retain the bioactivity of Tyr and enhance the sensitivity. The detection conditions, including working potential, pH of supporting electrolyte and the amount of Tyr were optimumed. As an example, the biosensor for catechol determination displayed a linear range of 8.0 × 10 to 2.0 × 10 mol·L with a detection limit of 4.5 × 10 mol·L (S/ = 3). This method has a rapid response time within 10 s, and shows excellent repeatability and stability. Moreover, the resulting biosensor could be disposable, low-cost, reliable and easy to carry. This kind of new Tyr biosensor provides great potential for rapid, on-site and cost-effective analysis of phenolic contaminants in environmental water samples.

关键词: on-site determination     tyrosinase biosensor     phenolic contaminants     single-walled carbon nanotubes     gold nanoparticles     screen-printed electrodes    

Winner determination problem with loss-averse buyers in reverse auctions

Xiaohu QIAN, Min HUANG, Yangyang YU, Xingwei WANG

《工程管理前沿(英文)》 2017年 第4卷 第2期   页码 212-220 doi: 10.15302/J-FEM-2017019

摘要: Reverse auctions have been widely adopted for purchasing goods and services. This paper considers a novel winner determination problem in a multiple-object reverse auction in which the buyer involves loss-averse behavior due to uncertain attributes. A corresponding winner determination model based on cumulative prospect theory is proposed. Due to the NP-hard characteristic, a loaded route strategy is proposed to ensure the feasibility of the model. Then, an improved ant colony algorithm that consists of a dynamic transition strategy and a Max-Min pheromone strategy is designed. Numerical experiments are conducted to illustrate the effectiveness of the proposed model and algorithm. We find that under the loaded route strategy, the improved ant colony algorithm performs better than the basic ant colony algorithm. In addition, the proposed model can effectively characterize the buyer’s loss-averse behavior.

关键词: reverse auction     loss aversion     winner determination     improved ant colony algorithm    

Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

《农业科学与工程前沿(英文)》 2017年 第4卷 第1期   页码 121-122 doi: 10.15302/J-FASE-2017142

Experimental determination of Cd

Farhah Amalya ISMAIL, Ahmad Zaharin ARIS

《环境科学与工程前沿(英文)》 2013年 第7卷 第3期   页码 356-364 doi: 10.1007/s11783-013-0488-1

摘要: Carbonate shells have an astonishing ability in the removal of Cd in a short time period with emphasis on being a low cost adsorbent. In the present study, the sorption capacity of carbonate shells was studied for Cd in batch experiments. The influence of different carbonate shell sizes and physico-chemical factors were evaluated and the results were analyzed for its correlation matrices by using Predictive Analytics Software (PASW). The mineralogy state of aqueous solution regarding the saturation index was simulated using PHREEQC to identify the Cd uptake mechanism. The Cd uptake rates were calculated as well as Ca , concentration, pH, ambient humidity and temperature were measured. Cd removal of 91.52% was achieved after 5 h adsorption. The adsorption efficiencies were significantly influenced by pH as they increased with the increase of pH from acidic solution (5.50±0.02) to slightly alkaline (7.60±0.05). In addition, the mineralogy state of aqueous solution calculated from PHREEQC confirmed that the increment of Ca and concentrations in solution was attributed to the dissolution of carbonate shells. Moreover, the ion exchange adsorption mechanism of Cd toward Ca was identified as the process involved in Cd uptake.

关键词: carbonate shell     cadmium     heavy metal     adsorption mechanism     saturation state    

A simplified method for the determination of vertically loaded pile-soil interface parameters in layered

Jiu-jiang WU,Yan LI,Qian-gong CHENG,Hua WEN,Xin LIANG

《结构与土木工程前沿(英文)》 2016年 第10卷 第1期   页码 103-111 doi: 10.1007/s11709-015-0328-4

摘要: The numerical analysis of pile-soil interaction commonly requires a lot of trial works to determine the interface parameters and the accuracy cannot be ensured normally. Considering this, this paper first conducts a sensitivity analysis to figure out the influence of interface parameters on the bearing behavior of a single pile in sand. Then, a simplified method for the determination of pile-soil interface parameters in layered soil is proposed based on the parameter studies. Finally, a filed loading test is used for the validation of the simplified method, and the calculated results agree well with the monitoring data. In general, the simplified method proposed in this paper works with higher accuracy and consumes less time compared with the traditional trial works, especially on the determinations of interfacial cohesive and interfacial friction angle.

关键词: determination of interface parameters     pile-soil interaction     FLAC3D     sensitivity analysis     layered soil    

Erratum to: Optimized determination of airborne tetracycline resistance genes in laboratory atmosphere

Lu Song, Can Wang, Yizhu Wang

《环境科学与工程前沿(英文)》 2020年 第14卷 第6期 doi: 10.1007/s11783-020-1289-y

Build orientation determination of multi-feature mechanical parts in selective laser melting via multi-objective

《机械工程前沿(英文)》 2023年 第18卷 第2期 doi: 10.1007/s11465-022-0737-8

摘要: Selective laser melting (SLM) is a unique additive manufacturing (AM) category that can be used to manufacture mechanical parts. It has been widely used in aerospace and automotive using metal or alloy powder. The build orientation is crucial in AM because it affects the as-built part, including its part accuracy, surface roughness, support structure, and build time and cost. A mechanical part is usually composed of multiple surface features. The surface features carry the production and design knowledge, which can be utilized in SLM fabrication. This study proposes a method to determine the build orientation of multi-feature mechanical parts (MFMPs) in SLM. First, the surface features of an MFMP are recognized and grouped for formulating the particular optimization objectives. Second, the estimation models of involved optimization objectives are established, and a set of alternative build orientations (ABOs) is further obtained by many-objective optimization. Lastly, a multi-objective decision making method integrated by the technique for order of preference by similarity to the ideal solution and cosine similarity measure is presented to select an optimal build orientation from those ABOs. The weights of the feature groups and considered objectives are achieved by a fuzzy analytical hierarchy process. Two case studies are reported to validate the proposed method with numerical results, and the effectiveness comparison is presented. Physical manufacturing is conducted to prove the performance of the proposed method. The measured average sampling surface roughness of the most crucial feature of the bracket in the original orientation and the orientations obtained by the weighted sum model and the proposed method are 15.82, 10.84, and 10.62 μm, respectively. The numerical and physical validation results demonstrate that the proposed method is desirable to determine the build orientations of MFMPs with competitive results in SLM.

关键词: selective laser melting (SLM)     build orientation determination     multi-feature mechanical part (MFMP)     fuzzy analytical hierarchy process     multi-objective decision making (MODM)    

Optimized determination of airborne tetracycline resistance genes in laboratory atmosphere

Lu Song, Can Wang, Yizhu Wang

《环境科学与工程前沿(英文)》 2020年 第14卷 第6期 doi: 10.1007/s11783-020-1274-5

摘要: Abstract • Sampling parameters with high efficiency was determined. • Operational process to detect airborne ARGs was optimized. • Providing research basis to control airborne ARGs of a laboratory atmosphere Antibiotic resistance genes (ARGs) have been detected in various atmospheric environments. Airborne ARGs transmission presents the public health threat. However, it is very difficult to quantify airborne ARGs because of the limited availability of collectable airborne particulate matter and the low biological content of samples. In this study, an optimized protocol for collecting and detecting airborne ARGs was presented. Experimental results showed that recovery efficiency tended to increase initially and then declined over time, and a range of 550–780 copies/mm2 of capture loading was recommended to ensure that the recovery efficiency is greater than 75%. As the cell walls were mechanically disrupted and nucleic acids were released, the buffer wash protects ARGs dissolution. Three ratios of buffer volume to membrane area in buffer wash were compared. The highest concentrations of airborne ARGs were detected with 1.4 µL/mm2 buffer wash. Furthermore, the majority of the cells were disrupted by an ultrasonication pretreatment (5 min), allowing the efficiency ARGs detection of airborne samples. While, extending the ultrasonication can disrupt cell structures and gene sequence was broken down into fragments. Therefore, this study could provide a theoretical basis for the efficient filter collection of airborne ARGs in different environments. An optimized sampling method was proposed that the buffer wash was 1.4 µL/mm2 and the ultrasonication duration was 5 min. The indoor airborne ARGs were examined in accordance with the improved protocol in two laboratories. The result demonstrated that airborne ARGs in an indoor laboratory atmosphere could pose the considerable health risk to inhabitants and we should pay attention to some complicated indoor air environment.

关键词: Airborne tetracycline resistance genes     Filter sampling     Capture loading     Membrane pretreatment     Indoor laboratory atmosphere    

Intelligent methods for the process parameter determination of plastic injection molding

Huang GAO, Yun ZHANG, Xundao ZHOU, Dequn LI

《机械工程前沿(英文)》 2018年 第13卷 第1期   页码 85-95 doi: 10.1007/s11465-018-0491-0

摘要:

Injection molding is one of the most widely used material processing methods in producing plastic products with complex geometries and high precision. The determination of process parameters is important in obtaining qualified products and maintaining product quality. This article reviews the recent studies and developments of the intelligent methods applied in the process parameter determination of injection molding. These intelligent methods are classified into three categories: Case-based reasoning methods, expert system-based methods, and data fitting and optimization methods. A framework of process parameter determination is proposed after comprehensive discussions. Finally, the conclusions and future research topics are discussed.

关键词: injection molding     intelligent methods     process parameters     optimization    

Determination and occurrence of endocrine disrupting compounds, pharmaceuticals and personal care products

Yong YU,Laosheng WU

《环境科学与工程前沿(英文)》 2015年 第9卷 第3期   页码 475-481 doi: 10.1007/s11783-014-0640-6

摘要: Endocrine disrupting compounds (EDCs), pharmaceuticals and personal care products (PPCPs) have attracted much attention due to widespread contamination in aquatic environment. In this study, we determined 13 EDCs and PPCPs in fish blood, bile and muscle by using gas chromatography-mass spectrometry (GC-MS). The limits of quantitation (LOQ) were in the ranges of 0.23–2.54, 0.22–2.36 ng·mL , and 0.24–2.57 ng·g dry weight (dw) for fish blood, bile and muscle, respectively. Recoveries of target compounds spiked into sample matrices and passed through the entire analytical procedure ranged from 65% to 95%, from 60% to 92% and from 62% to 91% for blood, bile and muscle, respectively. The methods were applied to the analysis of fish from a lake in California. Target compounds were relatively low in bile, and only bisphenol A (BPA) and diclofenac were measurable near the LOQ. Seven of 13 compounds were detected in blood, with total concentrations up to 39 ng·mL . Only BPA was frequently found in muscle, with mean concentration of 7.26 ng·g dw. The estimated daily intake of BPA through fish consumption for U.S. resident was significantly lower than the tolerable daily intake recommended by the European Food Safety Authority. This study showed that the exposure to the bisphenol A from fish diet is unlikely to pose a health risk.

关键词: endocrine disrupting compounds (EDCs)     pharmaceuticals and personal care products (PPCPs)     fish     bisphenol A (BPA)     risk assessment    

SPT based determination of undrained shear strength: Regression models and machine learning

Walid Khalid MBARAK, Esma Nur CINICIOGLU, Ozer CINICIOGLU

《结构与土木工程前沿(英文)》 2020年 第14卷 第1期   页码 185-198 doi: 10.1007/s11709-019-0591-x

摘要: The purpose of this study is the accurate prediction of undrained shear strength using Standard Penetration Test results and soil consistency indices, such as water content and Atterberg limits. With this study, along with the conventional methods of simple and multiple linear regression models, three machine learning algorithms, random forest, gradient boosting and stacked models, are developed for prediction of undrained shear strength. These models are employed on a relatively large data set from different projects around Turkey covering 230 observations. As an improvement over the available studies in literature, this study utilizes correct statistical analyses techniques on a relatively large database, such as using a train/test split on the data set to avoid overfitting of the developed models. Furthermore, the validity and consistency of the prediction results are ensured with the correct use of statistical measures like -value and cross-validation which were missing in previous studies. To compare the performances of the models developed in this study with the prior ones existing in literature, all models were applied on the test data set and their performances are evaluated in terms of the resulting root mean squared error ( ) values and coefficient of determination ( ). Accordingly, the models developed in this study demonstrate superior prediction capabilities compared to all of the prior studies. Moreover, to facilitate the use of machine learning algorithms for prediction purposes, entire source code prepared for this study and the collected data set are provided as supplements of this study.

关键词: undrained shear strength     linear regression     random forest     gradient boosting     machine learning     standard penetration test    

Improved genetic algorithm and its application to determination of critical slip surface with arbitrary

LI Liang, CHI Shichun, LIN Gao, CHENG Yungming

《结构与土木工程前沿(英文)》 2008年 第2卷 第2期   页码 145-150 doi: 10.1007/s11709-008-0016-8

摘要: In order to overcome the problem of being trapped by the local minima encountered in applying the simple genetic algorithm (GA) to search the critical slip surface of the slope, an improved procedure based on the harmony search algorithm is proposed. In the searching computation, the new solutions are obtained from the whole information of the current generation. The proposed method may be applied to calculate the minimum factors of safety of two complicated soil slopes. Comparison of the results with existing examples given by other authors has shown that the proposed method is feasible for stability analysis of soil slopes.

关键词: information     algorithm     Comparison     generation     feasible    

A study on rapid acid chrome black (MB 7) spectrophotometric determination of ClO

Jing DONG, Huilong WANG

《化学科学与工程前沿(英文)》 2011年 第5卷 第2期   页码 245-251 doi: 10.1007/s11705-010-1003-x

摘要: Experiments were conducted to investigate the degradation of 2,6-dinitro- -cresol (DNPC) in the chlorine dioxide (ClO ) catalytic oxidation process. Pure aluminum oxide was used as the catalyst in this process. The degradation of DNPC by ClO using aluminum oxide as catalyst was systematically studied by varying the experimental parameters, such as pH values, catalyst dosage, the initial concentration of DNPC and ClO , reaction time, etc. Under optimal condition (DNPC concentration 39 mg·L , ClO concentration 0.234 g·L , reaction time 15 min, catalyst dosage 4.7 g·L and pH 4.32), almost complete degradation of DNPC can be achieved. The kinetic studies revealed that the ClO catalytic oxidation degradation of DNPC followed pseudo-first-order kinetics with respect to both ClO and DNPC concentration. The repetitive use of the catalyst was investigated along sequential feed-batch trials. The catalyst performed efficiently after five runs. In addition, a simple and convenient method for the determination of ClO in water was developed by using acid chrome black 7 (MB 7) spectrophotometry in this paper.

关键词: chlorine dioxide     2     6-dinitro-p-cresol (DNPC)     aluminum oxide     water treatment     MB 7 spectrophotometry    

A novel flavonol-based colorimetric and turn-on fluorescent probe for rapid determination of hydrazine

《化学科学与工程前沿(英文)》 2023年 第17卷 第1期   页码 24-33 doi: 10.1007/s11705-022-2171-1

摘要: Hydrazine is extremely toxic and causes severe harm to human body. Herein, a novel fluorescent probe 4-oxo-2-styryl-4H-chromen-3-yl thiophene-2-carboxylate (FHT) was synthesized for detecting hydrazine by using natural cinnamaldehyde as starting material. This probe exhibited significantly enhanced fluorescence response towards hydrazine over various common metal ions, anions, and amine compounds. The detection limit of probe FHT for hydrazine was as low as 0.14 μmol·L–1, significantly lower than that of the threshold value of 0.312 μmol·L–1, imposed by the Environmental Protection Agency. Moreover, the proposed probe was able to detect hydrazine within wide pH (5–10) and linear detection ranges (0–110 μmol·L–1). This probe was employed for determining trace hydrazine in different environmental water samples. The probe FHT-loaded filter paper strips were able to conveniently detect hydrazine of low concentration through distinct naked-eye and fluorescent color changes. Importantly, the probe FHT with low cytotoxicity was successfully applied to visualize hydrazine in living Hela cells and zebrafish.

关键词: cinnamaldehyde     3-hydroxychromone derivative     hydrazine     fluorescent probe    

标题 作者 时间 类型 操作

Progress in studies of sex determination mechanisms and sex control techniques in

Qian ZHOU,Songlin CHEN

期刊论文

Rapid method for on-site determination of phenolic contaminants in water using a disposable biosensor

Yuanting LI, Dawei LI, Wei SONG, Meng LI, Jie ZOU, Yitao LONG

期刊论文

Winner determination problem with loss-averse buyers in reverse auctions

Xiaohu QIAN, Min HUANG, Yangyang YU, Xingwei WANG

期刊论文

Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

期刊论文

Experimental determination of Cd

Farhah Amalya ISMAIL, Ahmad Zaharin ARIS

期刊论文

A simplified method for the determination of vertically loaded pile-soil interface parameters in layered

Jiu-jiang WU,Yan LI,Qian-gong CHENG,Hua WEN,Xin LIANG

期刊论文

Erratum to: Optimized determination of airborne tetracycline resistance genes in laboratory atmosphere

Lu Song, Can Wang, Yizhu Wang

期刊论文

Build orientation determination of multi-feature mechanical parts in selective laser melting via multi-objective

期刊论文

Optimized determination of airborne tetracycline resistance genes in laboratory atmosphere

Lu Song, Can Wang, Yizhu Wang

期刊论文

Intelligent methods for the process parameter determination of plastic injection molding

Huang GAO, Yun ZHANG, Xundao ZHOU, Dequn LI

期刊论文

Determination and occurrence of endocrine disrupting compounds, pharmaceuticals and personal care products

Yong YU,Laosheng WU

期刊论文

SPT based determination of undrained shear strength: Regression models and machine learning

Walid Khalid MBARAK, Esma Nur CINICIOGLU, Ozer CINICIOGLU

期刊论文

Improved genetic algorithm and its application to determination of critical slip surface with arbitrary

LI Liang, CHI Shichun, LIN Gao, CHENG Yungming

期刊论文

A study on rapid acid chrome black (MB 7) spectrophotometric determination of ClO

Jing DONG, Huilong WANG

期刊论文

A novel flavonol-based colorimetric and turn-on fluorescent probe for rapid determination of hydrazine

期刊论文