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Monocyte subsets and their differentiation tendency after burn injury

null

《医学前沿（英文）》 2013年 第7卷 第4期   页码 397-400 doi: 10.1007/s11684-013-0298-7

摘要：

Monocytes are critical effectors and regulators of immune response. Studying the nomenclature of monocyte subsets may be beneficial for understanding the complex function of monocytes in steady and inflammatory states. A monocyte has the potential to differentiate into dendritic cells or macrophages, and this behavior significantly changes in severely burned patients and mice. The findings in the present study may help enhance understanding on the perturbation of the immune system after severe burn injury.

关键词： monocyte     differentiation     burn    

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GID complex regulates the differentiation of neural stem cells by destabilizing TET2

《医学前沿（英文）》 doi: 10.1007/s11684-023-1007-9

摘要： Brain development requires a delicate balance between self-renewal and differentiation in neural stem cells (NSC), which rely on the precise regulation of gene expression. Ten-eleven translocation 2 (TET2) modulates gene expression by the hydroxymethylation of 5-methylcytosine in DNA as an important epigenetic factor and participates in the neuronal differentiation. Yet, the regulation of TET2 in the process of neuronal differentiation remains unknown. Here, the protein level of TET2 was reduced by the ubiquitin-proteasome pathway during NSC differentiation, in contrast to mRNA level. We identified that TET2 physically interacts with the core subunits of the glucose-induced degradation-deficient (GID) ubiquitin ligase complex, an evolutionarily conserved ubiquitin ligase complex and is ubiquitinated by itself. The protein levels of GID complex subunits increased reciprocally with TET2 level upon NSC differentiation. The silencing of the core subunits of the GID complex, including WDR26 and ARMC8, attenuated the ubiquitination and degradation of TET2, increased the global 5-hydroxymethylcytosine levels, and promoted the differentiation of the NSC. TET2 level increased in the brain of the Wdr26+/− mice. Our results illustrated that the GID complex negatively regulates TET2 protein stability, further modulates NSC differentiation, and represents a novel regulatory mechanism involved in brain development.

关键词： TET2     GID complex     neural stem cells     differentiation of neurons    

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Abivertinib inhibits megakaryocyte differentiation and platelet biogenesis

《医学前沿（英文）》 2022年 第16卷 第3期   页码 416-428 doi: 10.1007/s11684-021-0838-5

摘要： Abivertinib, a third-generation tyrosine kinase inhibitor, is originally designed to target epidermal growth factor receptor (EGFR)-activating mutations. Previous studies have shown that abivertinib has promising antitumor activity and a well-tolerated safety profile in patients with non-small-cell lung cancer. However, abivertinib also exhibited high inhibitory activity against Bruton’s tyrosine kinase and Janus kinase 3. Given that these kinases play some roles in the progression of megakaryopoiesis, we speculate that abivertinib can affect megakaryocyte (MK) differentiation and platelet biogenesis. We treated cord blood CD34+ hematopoietic stem cells, Meg-01 cells, and C57BL/6 mice with abivertinib and observed megakaryopoiesis to determine the biological effect of abivertinib on MK differentiation and platelet biogenesis. Our in vitro results showed that abivertinib impaired the CFU-MK formation, proliferation of CD34+ HSC-derived MK progenitor cells, and differentiation and functions of MKs and inhibited Meg-01-derived MK differentiation. These results suggested that megakaryopoiesis was inhibited by abivertinib. We also demonstrated in vivo that abivertinib decreased the number of MKs in bone marrow and platelet counts in mice, which suggested that thrombopoiesis was also inhibited. Thus, these preclinical data collectively suggested that abivertinib could inhibit MK differentiation and platelet biogenesis and might be an agent for thrombocythemia.

关键词： abivertinib     Btk inhibitor     platelet     megakaryocyte     megakaryopoiesis     thrombopoiesis    

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The secondary laticifer differentiation in rubber tree is induced by trichostatin A, an inhibitor of

Shixin ZHANG,Shaohua WU,Weimin TIAN

《农业科学与工程前沿（英文）》 2016年 第3卷 第4期   页码 357-362 doi: 10.15302/J-FASE-2016125

摘要： The secondary laticifer, a specific tissue in the secondary phloem of rubber tree, is differentiated from the vascular cambia. The number of the secondary laticifer in the trunk bark of rubber tree is positively correlated with rubber yield. Although jasmonates have been demonstrated to be crucial in the regulation of secondary laticifer differentiation, the mechanism for the jasmonate-induced secondary laticifer differentiation remains to be elucidated. By using an experimental morphological technique, the present study revealed that trichostatin A (TSA), an inhibitor of histone deacetylation, could induce the secondary laticifer differentiation in a concentration-dependent manner. The results suggest that histone acetylation is essential for the secondary laticifer differentiation in rubber tree.

关键词： Hevea brasiliensis     histone acetylation     laticifer differentiation     trichostatin     vascular cambia    

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Tank-dependence of the functionality and network differentiation of activated sludge community in a full-scale

《环境科学与工程前沿（英文）》 2023年 第17卷 第3期 doi: 10.1007/s11783-023-1636-x

摘要：

● Environmental parameters affected functional bacteria and network associations.

关键词： Activated sludge     Bacterial community     Tank-dependence     Network association     Functional bacteria    

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coupling relationship of industrial agglomeration and low-carbon economy considering spatiotemporal differentiation

《工程管理前沿（英文）》 2023年 第10卷 第2期   页码 285-299 doi: 10.1007/s42524-022-0197-1

摘要： Although China’s construction machinery thrives to meet the needs of construction, a number of challenges still remain to be overcome, such as lack of thorough knowledge of regional disparities and several limitations in terms of carbon emissions and economic development. Meanwhile, a low-carbon economy was proposed and implemented in China. This research aims to investigate the differences in industrial agglomeration of construction machineries and further explore the relationship between industrial agglomeration and low-carbon economy. On this basis, spatiotemporal analysis was performed to evaluate the levels of industrial agglomeration in different regions based on the situations of China’s construction machinery industry. Furthermore, this study explored the interaction between industrial agglomeration and low-carbon economy utilizing the coupling coordination analysis method. Results showed that the coupling coordination of the two subsystems was extremely unbalanced in 2006, and it maintained an increasing trend, reaching a relatively high level in 2018. Finally, suggestions, such as establishing a policy guarantee system and implementing variable policies in different regions, were proposed to provide guidelines for the government decision-making and promote the sustainable development of China’s construction machinery industry.

关键词： spatiotemporal differentiation     industrial agglomeration     low-carbon economy     construction machinery industry     empirical study    

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Repression of CDKN2C caused by PML/RARα binding promotes the proliferation and differentiation block

null

《医学前沿（英文）》 2016年 第10卷 第4期   页码 420-429 doi: 10.1007/s11684-016-0478-3

摘要：

Inappropriate cell proliferation during oncogenesis is often accompanied by inactivation of components involved in the cell cycle machinery. Here, we report that cyclin-dependent kinase inhibitor 2C (CDKN2C) as a member of the cyclin-dependent kinase inhibitors is a target of the PML/RARα oncofusion protein in leukemogenesis of acute promyelocytic leukemia (APL). We found that CDKN2C was markedly downregulated in APL blasts compared with normal promyelocytes. Chromatin immunoprecipitation combined with quantitative polymerase chain reaction demonstrated that PML/RARα directly bound to the CDKN2C promoter in the APL patient-derived cell line NB4. Luciferase assays indicated that PML/RARα inhibited the CDKN2C promoter activity in a dose-dependent manner. Furthermore, all-trans retinoic acid treatment induced CDKN2C expression by releasing the PML/RARα binding on chromatin in NB4 cells. Functional studies showed that ectopic expression of CDKN2C induced a cell cycle arrest at the G0/G1 phase and a partial differentiation in NB4 cells. Finally, the transcriptional regulation of CDKN2C was validated in primary APL patient samples. Collectively, this study highlights the importance of CDKN2C inactivation in the abnormal cell cycle progression and differentiation block of APL cells and may provide new insights into the study of pathogenesis and targeted therapy of APL.

关键词： CDKN2C     acute promyelocytic leukemia     cell cycle arrest     differentiation    

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Cannabidiol prevents depressive-like behaviors through the modulation of neural stem cell differentiation

《医学前沿（英文）》 2022年 第16卷 第2期   页码 227-239 doi: 10.1007/s11684-021-0896-8

摘要： Chronic stress impairs radial neural stem cell (rNSC) differentiation and adult hippocampal neurogenesis (AHN), whereas promoting AHN can increase stress resilience against depression. Therefore, investigating the mechanism of neural differentiation and AHN is of great importance for developing antidepressant drugs. The nonpsychoactive phytocannabinoid cannabidiol (CBD) has been shown to be effective against depression. However, whether CBD can modulate rNSC differentiation and hippocampal neurogenesis is unknown. Here, by using the chronic restraint stress (CRS) mouse model, we showed that hippocampal rNSCs mostly differentiated into astrocytes under stress conditions. Moreover, transcriptome analysis revealed that the FoxO signaling pathway was involved in the regulation of this process. The administration of CBD rescued depressive-like symptoms in CRS mice and prevented rNSCs overactivation and differentiation into astrocyte, which was partly mediated by the modulation of the FoxO signaling pathway. These results revealed a previously unknown neural mechanism for neural differentiation and AHN in depression and provided mechanistic insights into the antidepressive effects of CBD.

关键词： cannabidiol     depression     radial neural stem cells     neurogenesis    

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Industrial eco-efficiency and its spatial-temporal differentiation in China

Wei YANG, Fengjun JIN, Chengjin WANG, Chen LV

《环境科学与工程前沿（英文）》 2012年 第6卷 第4期   页码 559-568 doi: 10.1007/s11783-012-0400-4

摘要： The aim of this paper is to study the spatial-temporal differentiation of industrial eco-efficiency in China. Using methods based on the data envelopment analysis (DEA) model and exploratory spatial data analysis (ESDA) and data from 1985, 1995, 2005, and 2008 of 30 provinces in China, the spatial-temporal pattern changes in industrial eco-efficiency are discussed. The results show that: first, the patterns of industrial eco-efficiency are dominated by clustering of relatively low efficiency provinces; second, spatial relationships between the industrial eco-efficiencies of different provinces changed slightly throughout the period and the provinces persistently exhibit spatial concentration of relatively low industrial eco-efficiency; finally, there is an obvious trend in the polarization of industrial eco-efficiency, i.e., the higher level spatial units are concentrated in eastern China, and the lower level spatial units are mainly in western and central China.

关键词： industrial eco-efficiency     data envelopment analysis (DEA) model     exploratory spatial data analysis (ESDA)    

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辨证论治艾滋病

王文奎,王峰,王 岭

《中国工程科学》 2007年 第9卷 第8期   页码 30-34

摘要：

艾滋病是一种获得性免疫缺陷综合征，中医学的观点则是正气严重不足。正气依靠精神的支撑，而精神来源于饮食气味及其精微物质 ，精微物质的转化主要靠脏腑功能的正常运化。脏腑功能活动是生命运动的根本。五脏各有所主，各司其职，并且相互和合与平衡，只有当五 脏功能旺盛，并且相互和合时，人体的生命力才会强大，机体“正气”充足，才会有免疫力。在五脏之中，肝、脾、肾三脏功能要保持平衡， 其他各脏功能才会自然趋向平衡与协调，人体才会健康长寿，这就是“三维守恒”法则。

关键词： 艾滋病     正气亏损     辨证论治     五脏功能     三维守恒    

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Resveratrol promotes the survival and neuronal differentiation of hypoxia-conditioned neuronal progenitor

Yao Yao, Rui Zhou, Rui Bai, Jing Wang, Mengjiao Tu, Jingjing Shi, Xiao He, Jinyun Zhou, Liu Feng, Yuanxue Gao, Fahuan Song, Feng Lan, Xingguo Liu, Mei Tian, Hong Zhang

《医学前沿（英文）》 2021年 第15卷 第3期   页码 472-485 doi: 10.1007/s11684-021-0832-y

摘要： Hypoxia conditioning could increase the survival of transplanted neuronal progenitor cells (NPCs) in rats with cerebral ischemia but could also hinder neuronal differentiation partly by suppressing mitochondrial metabolism. In this work, the mitochondrial metabolism of hypoxia-conditioned NPCs (hcNPCs) was upregulated via the additional administration of resveratrol, an herbal compound, to resolve the limitation of hypoxia conditioning on neuronal differentiation. Resveratrol was first applied during the neuronal differentiation of hcNPCs and concurrently promoted the differentiation, synaptogenesis, and functional development of neurons derived from hcNPCs and restored the mitochondrial metabolism. Furthermore, this herbal compound was used as an adjuvant during hcNPC transplantation in a photothrombotic stroke rat model. Resveratrol promoted neuronal differentiation and increased the long-term survival of transplanted hcNPCs. 18-fluorine fluorodeoxyglucose positron emission tomography and rotarod test showed that resveratrol and hcNPC transplantation synergistically improved the neurological and metabolic recovery of stroke rats. In conclusion, resveratrol promoted the neuronal differentiation and therapeutic efficiency of hcNPCs in stroke rats via restoring mitochondrial metabolism. This work suggested a novel approach to promote the clinical translation of NPC transplantation therapy.

关键词： neuronal progenitor cells     resveratrol     cerebral ischemia     neuronal differentiation     mitochondrial metabolism     positron emission tomography    

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Stem cell research in hepatocellular carcinoma

SUN Chengyi, ZUO Shi

《医学前沿（英文）》 2008年 第2卷 第1期   页码 1-4 doi: 10.1007/s11684-008-0001-6

摘要： The traditional view that adult human liver tumors, mainly hepatocellular carcinoma (HCC), arise from mature cell types has been challenged in recent decades. The results of several studies suggest that HCC can be derived from liver stem cells. There are four levels of cells in the liver stem cell lineage: hepatocytes, hepatic stem cells/oval cells, bone marrow stem cells and hepato-pancreas stem cells. However, whether HCC is resulted from the differentiation block of stem cells and, moreover, which liver stem cell lineage is the source cell of hepatocarcinogenesis remain controversial. In this review, we focus on the current status of liver stem cell research and their roles in carcinogenesis of HCC, in order to explore new approaches for stem cell therapy of HCC.

关键词： lineage     hepato-pancreas     differentiation block     carcinogenesis     current status    

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The role of microRNAs in adipocyte differentiation

null

《医学前沿（英文）》 2013年 第7卷 第2期   页码 223-230 doi: 10.1007/s11684-013-0252-8

摘要：

Adipocytes differentiate from mesenchymal stem cells (MSCs) in a process known as adipogenesis. The programme of adipogenesis is regulated by the sequential activation of transcription factors and several signaling pathways. There is growing evidence indicating that a class of small non-coding single-stranded RNAs known as “microRNAs (miRNAs)” also are involved in this process. In this review, we summarize the biology and functional mechanisms of miRNAs in adipocyte differentiation. In addition, we further discuss the miRNAs profiling, the miRNAs function and miRNAs target prediction in the adipogenesis.

关键词： microRNA     adipocyte     differentiation     adipogenesis    

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Developmental expression of CAPON and Dexras1 in spinal cord of rats

LI Xin, CHENG Chun, CHEN Mengling, GAO Shangfeng, ZHAO Jian, NIU Shuqiong, SHEN Aiguo

《医学前沿（英文）》 2008年 第2卷 第1期   页码 75-81 doi: 10.1007/s11684-008-0014-1

摘要： To study the expression of the carboxy-terminal PSD-95/DLG/ZO-1 ligand of nNOS (CAPON) and Dexras1 mRNA during development in the spinal cord of rats, real-time polymerase chain reaction (Real-time PCR), as a quantitative method, was used to study the developmental expression of CAPON and Dexras1 mRNA level in the spinal cord. The spatial expression of CAPON and Dexras1 mRNA was examined by a combination of hybridization (ISH) and immunofluorescence. During the development of the spinal cord, CAPON mRNA was expressed in low levels from embryo day 14 to day 18. At postnatal day 1, it reached the peak and was expressed in the part which will become the dorsal horn when mature. It then decreased gradually until postnatal week 12, when it presented in the ventral horn. At embryo day 14, Dexras1 mRNA was expressed at low levels, increased during embryo day 16 to day 18 and peaked at postnatal day 1. Spatiotemporal expression of Dexras1 mRNA was similar to CAPON as confirmed by correlation analysis and colocalization. CAPON and neuronal nitric oxide synthase (nNOS) was expressed within the same cells of the dorsal horn at postnatal day 1 but had different subcellular localizations. Co-expression of CAPON and Dexras1 mRNA in myeloid tissue during development process of rat indicates that the adaptor protein, CAPON may play a probable role in differentiation of neurons, synaptic plasticity and synaptogenesis by regulating nNOS to activate Dexras1.

关键词： CAPON     ISH     differentiation     postnatal     different subcellular    

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MicroRNAs and their roles in osteoclast differentiation

Zhuying Xia, Chao Chen, Peng Chen, Hui Xie, Xianghang Luo

《医学前沿（英文）》 2011年 第5卷 第4期   页码 414-419 doi: 10.1007/s11684-011-0168-0

摘要： Osteoclasts, which are derived from monocyte-macrophage precursors, are exclusive bone resorption cells. Limited evidence indicates that a class of small non-coding single-stranded RNAs known as “microRNAs” (miRNAs) is also involved in bone resorption. Although various miRNAs regulate cell proliferation and differentiation, few miRNAs have been reported to play a key role in the regulation of osteoclast differentiation. In this short review, the biology and functional mechanisms of miRNAs in osteoclastogenesis are summarized. The profiling, function, and target prediction of miRNAs are discussed as well.

关键词： miRNA     osteoclast differentiation     antagomirs    

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标题 作者 时间 类型 操作

Monocyte subsets and their differentiation tendency after burn injury

null

期刊论文

GID complex regulates the differentiation of neural stem cells by destabilizing TET2

期刊论文

Abivertinib inhibits megakaryocyte differentiation and platelet biogenesis

期刊论文

The secondary laticifer differentiation in rubber tree is induced by trichostatin A, an inhibitor of

Shixin ZHANG,Shaohua WU,Weimin TIAN

期刊论文

Tank-dependence of the functionality and network differentiation of activated sludge community in a full-scale

期刊论文

coupling relationship of industrial agglomeration and low-carbon economy considering spatiotemporal differentiation

期刊论文

Repression of CDKN2C caused by PML/RARα binding promotes the proliferation and differentiation block

null

期刊论文

Cannabidiol prevents depressive-like behaviors through the modulation of neural stem cell differentiation

期刊论文

Industrial eco-efficiency and its spatial-temporal differentiation in China

Wei YANG, Fengjun JIN, Chengjin WANG, Chen LV

期刊论文

辨证论治艾滋病

王文奎,王峰,王 岭

期刊论文

Resveratrol promotes the survival and neuronal differentiation of hypoxia-conditioned neuronal progenitor

Yao Yao, Rui Zhou, Rui Bai, Jing Wang, Mengjiao Tu, Jingjing Shi, Xiao He, Jinyun Zhou, Liu Feng, Yuanxue Gao, Fahuan Song, Feng Lan, Xingguo Liu, Mei Tian, Hong Zhang

期刊论文

Stem cell research in hepatocellular carcinoma

SUN Chengyi, ZUO Shi

期刊论文

The role of microRNAs in adipocyte differentiation

null

期刊论文

Developmental expression of CAPON and Dexras1 in spinal cord of rats

LI Xin, CHENG Chun, CHEN Mengling, GAO Shangfeng, ZHAO Jian, NIU Shuqiong, SHEN Aiguo

期刊论文

MicroRNAs and their roles in osteoclast differentiation

Zhuying Xia, Chao Chen, Peng Chen, Hui Xie, Xianghang Luo

期刊论文